Genetic dissection of regulation by a repressing and novel activating corrinoid riboswitch enables engineering of synthetic riboswitches [PDF]
mBio, 2023 The ability to sense and respond to intracellular metabolite levels enables cells to adapt to environmental conditions. Many prokaryotes use riboswitches—structured RNA elements usually located in the 5′ untranslated region of mRNAs—to sense ...
Rebecca R. Procknow +4 more
doaj +3 more sources
Cobalamin Riboswitches Are Broadly Sensitive to Corrinoid Cofactors to Enable an Efficient Gene Regulatory Strategy [PDF]
mBio, 2022 In bacteria, many essential metabolic processes are controlled by riboswitches, gene regulatory RNAs that directly bind and detect metabolites. Highly specific effector binding enables riboswitches to respond to a single biologically relevant metabolite.
Kristopher J. Kennedy +6 more
doaj +2 more sources
Insights into pyrrolysine function from structures of a trimethylamine methyltransferase and its corrinoid protein complex [PDF]
Communications Biology, 2023 Structures of Methanosarcina barkeri trimethylamine methyltransferase (MttB) with its substrates reveal the role of pyrrolysine in methyl group transfer from trimethylamine to the corrinoid cofactor in MttC.
Jiaxin Li +6 more
doaj +2 more sources
Structure of a membrane-bound menaquinol:organohalide oxidoreductase [PDF]
Nature Communications, 2023 Organohalide-respiring bacteria are key organisms for the bioremediation of soils and aquifers contaminated with halogenated organic compounds. The major players in this process are respiratory reductive dehalogenases, corrinoid enzymes that use ...
Lorenzo Cimmino +7 more
doaj +2 more sources
mibPOPdb: An online database for microbial biodegradation of persistent organic pollutants
iMeta, Volume 1, Issue 4, December 2022., 2022 This study presents the Microbial Biodegradation of Persistent Organic Pollutants Database (mibPOPdb) database, a web‐accessible literature‐based microbial biodegradation of persistent organic pollutants (POPs) resource. We also developed a robust chemical biodegradability prediction model with Graph Neural Networks.
Tanyaradzwa R. Ngara +2 more
wiley +1 more source
Water Science and Technology, 2022 Complete dechlorination of trichloroethene (TCE) by Dehalococcoides mccartyi is catalyzed by reductive dehalogenases (RDases), which possess cobalamin as the crucial cofactor. However, virtually all D. mccartyi isolated thus far are corrinoid auxotrophs.
Haijun Li +4 more
doaj +1 more source
Four families of folate-independent methionine synthases.
PLoS Genetics, 2021 Although most organisms synthesize methionine from homocysteine and methyl folates, some have "core" methionine synthases that lack folate-binding domains and use other methyl donors.
Morgan N Price +2 more
doaj +1 more source
Frontiers in Microbiology, 2020 Chlordecone (Kepone®) and γ-hexachlorocyclohexane (γ-HCH or lindane) have been used for decades in the French West Indies (FWI) resulting in long-term soil and water pollution. In a previous work, we have identified a new Citrobacter species (sp.86) that
Agnès Barbance +11 more
doaj +1 more source
The 380 kb pCMU01 plasmid encodes chloromethane utilization genes and redundant genes for vitamin B12- and tetrahydrofolate-dependent chloromethane metabolism in Methylobacterium extorquens CM4: a proteomic and bioinformatics study. [PDF]
PLoS ONE, 2013 Chloromethane (CH3Cl) is the most abundant volatile halocarbon in the atmosphere and contributes to the destruction of stratospheric ozone. The only known pathway for bacterial chloromethane utilization (cmu) was characterized in Methylobacterium ...
Sandro Roselli +3 more
doaj +1 more source
Frontiers in Microbiology, 2015 De novo corrinoid biosynthesis represents one of the most complicated metabolic pathways in nature. Organohalide-respiring bacteria (OHRB) have developed different strategies to deal with their need of corrinoid, as it is an essential cofactor of ...
Aamani eRupakula +6 more
doaj +1 more source