Novel Pathway for Corrinoid Compounds Production in Lactobacillus [PDF]
Frontiers in Microbiology, 2018 Vitamin B12 or cobalamin is an essential metabolite for humans, which makes it an interesting compound for many research groups that focus in different producer-strains synthesis pathways.
Andrea Carolina Torres +4 more
doaj +8 more sources
Genetic dissection of regulation by a repressing and novel activating corrinoid riboswitch enables engineering of synthetic riboswitches [PDF]
mBio, 2023 The ability to sense and respond to intracellular metabolite levels enables cells to adapt to environmental conditions. Many prokaryotes use riboswitches—structured RNA elements usually located in the 5′ untranslated region of mRNAs—to sense ...
Rebecca R. Procknow +4 more
doaj +2 more sources
Cobalamin Riboswitches Are Broadly Sensitive to Corrinoid Cofactors to Enable an Efficient Gene Regulatory Strategy [PDF]
mBio, 2022 In bacteria, many essential metabolic processes are controlled by riboswitches, gene regulatory RNAs that directly bind and detect metabolites. Highly specific effector binding enables riboswitches to respond to a single biologically relevant metabolite.
Kristopher J. Kennedy +6 more
doaj +2 more sources
Insights into pyrrolysine function from structures of a trimethylamine methyltransferase and its corrinoid protein complex [PDF]
Communications Biology, 2023 Structures of Methanosarcina barkeri trimethylamine methyltransferase (MttB) with its substrates reveal the role of pyrrolysine in methyl group transfer from trimethylamine to the corrinoid cofactor in MttC.
Jiaxin Li +6 more
doaj +2 more sources
Laboratory evolution of E. coli with a natural vitamin B12 analog reveals roles for cobamide uptake and adenosylation in methionine synthase-dependent growth [PDF]
Journal of BacteriologyBacteria encounter chemically similar nutrients in their environment, which impact their growth in distinct ways. Among such nutrients are cobamides, the structurally diverse family of cofactors related to vitamin B12 (cobalamin), which function as ...
Kenny C. Mok +3 more
doaj +2 more sources
Methanogenic activities signatures in broilers fed black soldier fly (Hermetia illucens) larvae-based diets [PDF]
Biochemistry and Biophysics ReportsMethanogenic archaea in the avian gut contribute to hydrogen turnover and thus play a role in fermentative efficiency and greenhouse gas emission. While black soldier fly larvae meal (BSFLM) has been explored as a replacement for fishmeal in broiler ...
Deborah Oluwaferanmi Ibiwoye +6 more
doaj +2 more sources
Genomic potential for mercury biotransformation in marine sediments across marginal slope to hadal zone [PDF]
Nature CommunicationsMercury accumulates in the deep sea, but its ecological impact on deep-sea ecosystems remains poorly understood. We conduct an analysis of 32 sediment cores, comprising 101 layers for the study of metagenomes, and additional 41 global reference sediment ...
Zhuobo Li +5 more
doaj +2 more sources
Skin-associated Corynebacterium amycolatum shares cobamides [PDF]
mSphereThe underlying interactions that occur to maintain skin microbiome composition, function, and overall skin health are largely unknown. Often, these types of interactions are mediated by microbial metabolites.
M. H. Swaney +4 more
doaj +2 more sources
Site-specific incorporation of 19F-nuclei at protein C-terminus to probe allosteric conformational transitions of metalloproteins [PDF]
Communications BiologyAllosteric conformational change is an important paradigm in the regulation of protein function, which is typically triggered by the binding of small cofactors, metal ions or protein partners.
Xichun Liu +6 more
doaj +2 more sources
Corrinoids in anaerobic bacteria [PDF]
FEMS Microbiology Letters, 1990 C1-metabolizing bacteria were analyzed for their corrinoids. The autotrophic phototrophe Chloroflexus aurantiacus contains predominantly the light-sensitive coenzyme B12. The corrinoid could be teh prostethic group of a methylmalonyl-CoA mutase, which is involved in the CO2 fixing reaction sequence from proplonyl-CoA to succinyl CoA.
Erhard Stupperich +2 more
openaire +2 more sources