Results 21 to 30 of about 2,324 (198)

The 380 kb pCMU01 plasmid encodes chloromethane utilization genes and redundant genes for vitamin B12- and tetrahydrofolate-dependent chloromethane metabolism in Methylobacterium extorquens CM4: a proteomic and bioinformatics study. [PDF]

PLoS ONE, 2013
Chloromethane (CH3Cl) is the most abundant volatile halocarbon in the atmosphere and contributes to the destruction of stratospheric ozone. The only known pathway for bacterial chloromethane utilization (cmu) was characterized in Methylobacterium ...
Sandro Roselli, Thierry Nadalig, Stéphane Vuilleumier, Françoise Bringel   +3 more
doaj   +1 more source

Structure of a membrane-bound menaquinol:organohalide oxidoreductase

Nature Communications, 2023
Organohalide-respiring bacteria are key organisms for the bioremediation of soils and aquifers contaminated with halogenated organic compounds. The major players in this process are respiratory reductive dehalogenases, corrinoid enzymes that use ...
Lorenzo Cimmino, Américo G. Duarte, Dongchun Ni, Babatunde E. Ekundayo, Inês A. C. Pereira, Henning Stahlberg, Christof Holliger, Julien Maillard   +7 more
doaj   +1 more source

Comparative Physiology and Genomics of Thermincola and Carboxydocella Strains and Description of Two Novel Isolates. [PDF]

Environ Microbiol
Two novel thermophilic bacteria, Thermincola strain AZ34E and Carboxydocella strain AZ29I, were isolated from hydrothermal environments and shown to oxidise carbon monoxide via the water‐gas shift reaction. Comparative genomic and phylogenetic analyses revealed that all currently described isolates within Thermincola and Carboxydocella each represent a
Galani A, Antony Venancius M, Sipkema D, Sousa DZ.   +3 more
europepmc   +2 more sources

Human gut Bacteroides capture vitamin B12 via cell surface-exposed lipoproteins

eLife, 2018
Human gut Bacteroides use surface-exposed lipoproteins to bind and metabolize complex polysaccharides. Although vitamins and other nutrients are also essential for commensal fitness, much less is known about how commensal bacteria compete with each other
Aaron G Wexler, Whitman B Schofield, Patrick H Degnan, Ewa Folta-Stogniew, Natasha A Barry, Andrew L Goodman   +5 more
doaj   +1 more source

Accurate prediction by AlphaFold2 for ligand binding in a reductive dehalogenase and implications for PFAS (per- and polyfluoroalkyl substance) biodegradation

Scientific Reports, 2023
Despite the success of AlphaFold2 (AF2), it is unclear how AF2 models accommodate for ligand binding. Here, we start with a protein sequence from Acidimicrobiaceae TMED77 (T7RdhA) with potential for catalyzing the degradation of per- and polyfluoroalkyl ...
Hao-Bo Guo, Vanessa A. Varaljay, Gary Kedziora, Kimberly Taylor, Sanaz Farajollahi, Nina Lombardo, Eric Harper, Chia Hung, Marie Gross, Alexander Perminov, Patrick Dennis, Nancy Kelley-Loughnane, Rajiv Berry   +12 more
doaj   +1 more source

Evidence for corrin biosynthesis in the last universal common ancestor. [PDF]

FEBS J
Cobalamin biosynthesis can be traced back to the last universal common ancestor. After a stage of chemical evolution of the acetyl‐CoA pathway, the enzymatic synthesis of cobalamin evolved, allowing life to be independent of the insoluble solid‐state catalysts that tethered primordial CO2 assimilation to the Earth's crust, suggesting a role for corrin ...
Modjewski LD, Karavaeva V, Mrnjavac N, Knopp M, Martin WF, Sousa FL.   +5 more
europepmc   +2 more sources

Examination of the Glycine Betaine-Dependent Methylotrophic Methanogenesis Pathway: Insights Into Anaerobic Quaternary Amine Methylotrophy

Frontiers in Microbiology, 2019
Recent studies indicate that environmentally abundant quaternary amines (QAs) are a primary source for methanogenesis, yet the catabolic enzymes are unknown.
Adam J. Creighbaum, Tomislav Ticak, Shrameeta Shinde, Xin Wang, Donald J. Ferguson, Donald J. Ferguson   +5 more
doaj   +1 more source

Versatility in Corrinoid Salvaging and Remodeling Pathways Supports Corrinoid-Dependent Metabolism in Dehalococcoides mccartyi [PDF]

Applied and Environmental Microbiology, 2012
ABSTRACTCorrinoids are cobalt-containing molecules that function as enzyme cofactors in a wide variety of organisms but are produced solely by a subset of prokaryotes. Specific corrinoids are identified by the structure of their axial ligands. The lower axial ligand of a corrinoid can be a benzimidazole, purine, or phenolic compound. Though it is known
Shan, Yi, Erica C, Seth, Yu-Jie, Men, Sally P, Stabler, Robert H, Allen, Lisa, Alvarez-Cohen, Michiko E, Taga   +6 more
openaire   +2 more sources

Exploitation of a Type 1 Toxin–Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen Eubacterium limosum

Microorganisms, 2023
Targeted mutations in the anaerobic methylotroph Eubacterium limosum have previously been obtained using CRISPR-based mutagenesis methods.
James Millard, Alexander Agius, Ying Zhang, Philippe Soucaille, Nigel Peter Minton   +4 more
doaj   +1 more source

Phylogenetic distribution and experimental characterization of corrinoid production and dependence in soil bacterial isolates. [PDF]

ISME J
Soil microbial communities impact carbon sequestration and release, biogeochemical cycling, and agricultural yields. These global effects rely on metabolic interactions that modulate community composition and function.
Alvarez-Aponte ZI, Govindaraju AM, Hallberg ZF, Nicolas AM, Green MA, Mok KC, Fonseca-García C, Coleman-Derr D, Brodie EL, Carlson HK, Taga ME.   +10 more
europepmc   +2 more sources