Results 41 to 50 of about 14,431 (222)

Biosynthesis of Chondroitin in Engineered Corynebacterium glutamicum

Journal of Microbiology and Biotechnology, 2019
Chondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum.
Fangyu, Cheng, Sijin, Luozhong, Huimin, Yu, Zhigang, Guo   +3 more
openaire   +3 more sources

Stable integration of the Mrx1-roGFP2 biosensor to monitor dynamic changes of the mycothiol redox potential in Corynebacterium glutamicum

Redox Biology, 2019
Mycothiol (MSH) functions as major low molecular weight (LMW) thiol in the industrially important Corynebacterium glutamicum. In this study, we genomically integrated an Mrx1-roGFP2 biosensor in C.
Quach Ngoc Tung, Vu Van Loi, Tobias Busche, Andreas Nerlich, Maren Mieth, Johanna Milse, Jörn Kalinowski, Andreas C. Hocke, Haike Antelmann   +8 more
doaj   +1 more source

Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine [PDF]

, 2012
Background: Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism.
Sarah Zimmet, Susanne Peifer, Elmar Heinzle, Heinzle, Elmar, Dietrich A Volmer, Schneider, Konstantin, Konstantin Schneider, Barduhn, Tobias, Volmer, Dietrich A., Tobias Barduhn, Zimmet, Sarah, Peifer, Susanne   +11 more
core   +1 more source

Lysine uptake and exchange in Corynebacterium glutamicum

Journal of Bacteriology, 1990
Resting cells of Corynebacterium glutamicum (ATCC 13032) accumulate [14C]lysine by a transport system with a relatively high affinity (10 microMs) and a low maximum velocity (0.15 nmol/min per mg [dry weight]). Uptake of lysine was not inhibited by uncouplers or by ionophores affecting the ion gradients and the energetic state of the cell.
S, Bröer, R, Krämer
openaire   +3 more sources

Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors. [PDF]

PLoS ONE, 2017
Corynebacterium glutamicum (C. glutamicum) is a favorable host cell for the production of recombinant proteins, such as important enzymes and pharmaceutical proteins, due to its excellent potential advantages.
Yang Sun, Wenwen Guo, Fen Wang, Chunjun Zhan, Yankun Yang, Xiuxia Liu, Zhonghu Bai   +6 more
doaj   +1 more source

Characterization of the biotin uptake system encoded by the biotin-inducible bioYMN operon of Corynebacterium glutamicum

, 2012
Schneider J, Peters-Wendisch P, Stansen KC, et al. Characterization of the biotin uptake system encoded by the biotin-inducible bioYMN operon of Corynebacterium glutamicum. BMC Microbiology.
Götker Susanne, Stanislav, Maximov, Schneider Jens, Stanislav Maximow, Stansen K Corinna, K Corinna Stansen, Susanne Götker, Reinhard Krämer, Peters-Wendisch Petra, Krämer, Reinhard, Schneider, Jens, Wendisch Volker F, Götker, Susanne, Wendisch, Volker F. ; https://orcid.org/, Volker F Wendisch, Petra Peters-Wendisch, Stansen, K. C., Jens Schneider, Krämer Reinhard, Maximow Stanislav, Peters-Wendisch, Petra   +20 more
core   +1 more source

Identifying the Growth Modulon of Corynebacterium glutamicum [PDF]

Frontiers in Microbiology, 2019
The growth rate (μ) of industrially relevant microbes, such as Corynebacterium glutamicum, is a fundamental property that indicates its production capacity. Therefore, understanding the mechanism underlying the growth rate is imperative for improving productivity and performance through metabolic engineering.
Thorsten Haas, Michaela Graf, Alexander Nieß, Tobias Busche, Tobias Busche, Jörn Kalinowski, Bastian Blombach, Bastian Blombach, Ralf Takors   +8 more
openaire   +4 more sources

A genome‐integrated CRISPR/Cas12a system enables efficient genetic engineering in Xenorhabdus budapestensis XBD8

New Plant Protection, EarlyView.
We developed a genome‐integrated CRISPR/Cas12a system for Xenorhabdus budapestensis XBD8, which enables precise deletion or replacement of DNA fragments ranging from 379 to 45,168 bp, with a success rate exceeding 90%. Application of this tool for the dynamic regulation of fabclavine‐8 biosynthesis resulted in a 1.90‐fold increase in production ...
Wenfeng Gan, Jiaqi Duan, Changyan Yin, Liwen Zhang, Guangyue Li   +4 more
wiley   +1 more source

The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriae and Corynebacterium jeikeium deduced from the complete genome sequences

, 2005
Brune I, Brinkrolf K, Kalinowski J, Pühler A, Tauch A. The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriae and Corynebacterium jeikeium ...
Brune, Iris, Brinkrolf, Karina, Tauch, Andreas, Kalinowski, Jörn, Pühler, Alfred ; https://orcid.org/   +4 more
core   +1 more source

Proteome Analysis of Corynebacterium diphtheriae–Macrophage Interaction

PROTEOMICS, EarlyView.
ABSTRACT Contact of Corynebacterium diphtheriae with macrophages induces adaptations on both bacterial and cellular sides. The study presented here was aiming to shed light on the simultaneous intracellular adaptation of the bacteria and changes in the proteome of the phagocytes in response to the internalization of C. diphtheriae.
Luca Musella, Jens Möller, Christopher Lischer, Julio Vera‐Gonzalez, Jörg Hofmann, Lisa Ott, Andreas Burkovski   +6 more
wiley   +1 more source