Results 41 to 50 of about 10,021 (182)

Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum [PDF]

BMC Microbiology, 2012
Abstract Background Corynebacterium glutamicum contains the glycosylated C50 carotenoid decaprenoxanthin as yellow pigment. Starting from isopentenyl pyrophosphate, which is generated in the non-mevalonate pathway, decaprenoxanthin is synthesized via the intermediates farnesyl pyrophosphate ...
Heider, Sabine, Peters-Wendisch, Petra, Wendisch, Volker F.   +2 more
openaire   +3 more sources

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, EarlyView.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

The Actinobacterium Corynebacterium glutamicum, an Industrial Workhorse

Journal of Microbiology and Biotechnology, 2016
Starting as a glutamate producer, Corynebacterium glutamicum has played a variety of roles in the industrial production of amino acids, one of the most important areas of white biotechnology. From shortly after its genome information became available, C.
Joo-Young, Lee, Yoon-Ah, Na, Eungsoo, Kim, Heung-Shick, Lee, Pil, Kim   +4 more
openaire   +2 more sources

Characterization of Xi-class mycothiol S-transferase from Corynebacterium glutamicum and its protective effects in oxidative stress

Microbial Cell Factories, 2019
Background Oxidative stress caused by inevitable hostile conditions during fermentative process was the most serious threat to the survival of the well-known industrial microorganism Corynebacterium glutamicum. To survive, C. glutamicum developed several
Meiru Si, Chengchuan Che, Guanxi Li, Xiaona Li, Zhijin Gong, Jinfeng Liu, Ge Yang, Can Chen   +7 more
doaj   +1 more source

Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum

Frontiers in Bioengineering and Biotechnology, 2020
Corynebacterium glutamicum is an important workhorse for industrial production of diversiform bioproducts. Multiplex control of metabolic pathway genes is crucial for maximizing biosynthesis of desired products.
Mingyue Li, Mingyue Li, Jiuzhou Chen, Yu Wang, Jiao Liu, Jingwen Huang, Jingwen Huang, Ning Chen, Ping Zheng, Jibin Sun   +9 more
doaj   +1 more source

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum‐free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m3), pharmaceutical‐grade amino acids and recombinant proteins remain major cost drivers.
Jelle C. Hoek, Jean‐Marc Daran, Marieke E. Klijn, Marcel A. Vieira‐Lara   +3 more
wiley   +1 more source

Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum

Nature Communications, 2020
The regulation of higher-order chromosome folding and segregation in bacteria is poorly understood. Here, Böhm et al. provide insights into the roles of DNA partitioning protein ParB and SMC condensin complexes in Corynebacterium glutamicum.
Kati Böhm, Giacomo Giacomelli, Andreas Schmidt, Axel Imhof, Romain Koszul, Martial Marbouty, Marc Bramkamp   +6 more
doaj   +1 more source

Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method

Microbial Cell Factories, 2023
Background Corynebacterium glutamicum has industrial track records for producing a variety of valuable products such as amino acids. Although CRISPR-based genome editing technologies have undergone immense developments in recent years, the suicide ...
Rui Su, Ting Wang, Taidong Bo, Ningyun Cai, Meng Yuan, Chen Wu, Hao Jiang, Huadong Peng, Ning Chen, Yanjun Li   +9 more
doaj   +1 more source

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, EarlyView.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Silencing of cryptic prophages inCorynebacterium glutamicum [PDF]

Nucleic Acids Research, 2016
DNA of viral origin represents a ubiquitous element of bacterial genomes. Its integration into host regulatory circuits is a pivotal driver of microbial evolution but requires the stringent regulation of phage gene activity. In this study, we describe the nucleoid-associated protein CgpS, which represents an essential protein functioning as a ...
Pfeifer, Eugen, Hünnefeld, Max, Popa, Ovidiu, Polen, Tino, Kohlheyer, Dietrich, Baumgart, Meike, Frunzke, Julia   +6 more
openaire   +3 more sources