Results 41 to 50 of about 27,404 (179)

A Futile Metabolic Cycle of Fatty Acyl Coenzyme A (Acyl-CoA) Hydrolysis and Resynthesis in Corynebacterium glutamicum and Its Disruption Leading to Fatty Acid Production [PDF]

, 2021
Fatty acyl coenzyme A (acyl-CoA) thioesterase (Tes) and acyl-CoA synthetase (FadD) catalyze opposing reactions between acyl-CoAs and free fatty acids. Within theIMPORTANCE The industrial amino acid producer Corynebacterium glutamicum has evolved into a ...
Takahashi, Keisuke, Ikeda, Masato, Hayashi, Mikiro, Imoto, Ryosuke, Ohtake, Tatsunori, Kawakami, Haruka, Takeno, Seiki   +6 more
core   +1 more source

Physiological changes and growth behavior of Corynebacterium glutamicum cells in biofilm

Frontiers in Microbiology, 2022
Biofilm cells are well-known for their increased survival and metabolic capabilities and have been increasingly implemented in industrial and biotechnological processes.
Di Zhang, Jiawen Shen, Xiwei Peng, Shansong Gao, Zhenyu Wang, Huifang Zhang, Wenjun Sun, Huanqing Niu, Huanqing Niu, Hanjie Ying, Hanjie Ying, Chenjie Zhu, Chenjie Zhu, Yong Chen, Yong Chen, Dong Liu, Dong Liu   +16 more
doaj   +1 more source

Induction and Enhancement of Bacteriocin Production (Review)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Stable integration of the Mrx1-roGFP2 biosensor to monitor dynamic changes of the mycothiol redox potential in Corynebacterium glutamicum

Redox Biology, 2019
Mycothiol (MSH) functions as major low molecular weight (LMW) thiol in the industrially important Corynebacterium glutamicum. In this study, we genomically integrated an Mrx1-roGFP2 biosensor in C.
Quach Ngoc Tung, Vu Van Loi, Tobias Busche, Andreas Nerlich, Maren Mieth, Johanna Milse, Jörn Kalinowski, Andreas C. Hocke, Haike Antelmann   +8 more
doaj   +1 more source

Isolation and Characterization of Random Citrate Synthase Variants That Increase Acetate Generation by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin, Ronnie L. Fulton, Abigail E. Rose, Mark A. Eiteman   +3 more
wiley   +1 more source

Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors. [PDF]

PLoS ONE, 2017
Corynebacterium glutamicum (C. glutamicum) is a favorable host cell for the production of recombinant proteins, such as important enzymes and pharmaceutical proteins, due to its excellent potential advantages.
Yang Sun, Wenwen Guo, Fen Wang, Chunjun Zhan, Yankun Yang, Xiuxia Liu, Zhonghu Bai   +6 more
doaj   +1 more source

Plant NAOGAT (ArgJ) Structure Reveals Mechanism of Ornithine Biosynthesis, Preference for Acetylornithine and Susceptibility to Mangotoxin

Plant, Cell &Environment, EarlyView.
ABSTRACT Arginine metabolism in plants is targeted by phytopathogens such as Pseudomonas syringae strains, which produce toxins that affect enzymes of plant arginine metabolism, causing devastating infections in crops. In this work, the structural and functional properties of plant N‐acetylornithine glutamate acetyltransferase (NAOGAT), the key ...
Maciej Nielipinski, Agnieszka J. Pietrzyk‐Brzezinska, Aleksandra Twarda‐Clapa, Bartosz Sekula   +3 more
wiley   +1 more source

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture

Biotechnology and Bioengineering, Volume 123, Issue 10, Page 2520-2535, October 2026.
ABSTRACT The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum‐free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m3), pharmaceutical‐grade amino acids and recombinant proteins remain major cost drivers.
Jelle C. Hoek, Jean‐Marc Daran, Marieke E. Klijn, Marcel A. Vieira‐Lara   +3 more
wiley   +1 more source

Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine [PDF]

, 2012
Background: Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism.
Sarah Zimmet, Susanne Peifer, Elmar Heinzle, Heinzle, Elmar, Dietrich A Volmer, Schneider, Konstantin, Konstantin Schneider, Barduhn, Tobias, Volmer, Dietrich A., Tobias Barduhn, Zimmet, Sarah, Peifer, Susanne   +11 more
core   +1 more source

Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method

Microbial Cell Factories, 2023
Background Corynebacterium glutamicum has industrial track records for producing a variety of valuable products such as amino acids. Although CRISPR-based genome editing technologies have undergone immense developments in recent years, the suicide ...
Rui Su, Ting Wang, Taidong Bo, Ningyun Cai, Meng Yuan, Chen Wu, Hao Jiang, Huadong Peng, Ning Chen, Yanjun Li   +9 more
doaj   +1 more source