Results 31 to 40 of about 10,021 (182)

Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in Corynebacterium glutamicum

Bioengineering, 2023
Corynebacterium glutamicum is regarded as an industrially important microbial cell factory and is widely used to produce various value-added chemicals. Because of the importance of C.
Nannan Zhao, Jian Wang, Aiqing Jia, Ying Lin, Suiping Zheng   +4 more
doaj   +1 more source

Engineering Corynebacterium glutamicum for isobutanol production [PDF]

Applied Microbiology and Biotechnology, 2010
The production of isobutanol in microorganisms has recently been achieved by harnessing the highly active 2-keto acid pathways. Since these 2-keto acids are precursors of amino acids, we aimed to construct an isobutanol production platform in Corynebacterium glutamicum, a well-known amino-acid-producing microorganism.
Smith, Kevin Michael, Cho, Kwang-Myung, Liao, James C.   +2 more
openaire   +4 more sources

Biosynthesis of Chondroitin in Engineered Corynebacterium glutamicum

Journal of Microbiology and Biotechnology, 2019
Chondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum.
Fangyu, Cheng, Sijin, Luozhong, Huimin, Yu, Zhigang, Guo   +3 more
openaire   +3 more sources

Physiological changes and growth behavior of Corynebacterium glutamicum cells in biofilm

Frontiers in Microbiology, 2022
Biofilm cells are well-known for their increased survival and metabolic capabilities and have been increasingly implemented in industrial and biotechnological processes.
Di Zhang, Jiawen Shen, Xiwei Peng, Shansong Gao, Zhenyu Wang, Huifang Zhang, Wenjun Sun, Huanqing Niu, Huanqing Niu, Hanjie Ying, Hanjie Ying, Chenjie Zhu, Chenjie Zhu, Yong Chen, Yong Chen, Dong Liu, Dong Liu   +16 more
doaj   +1 more source

Lysine uptake and exchange in Corynebacterium glutamicum

Journal of Bacteriology, 1990
Resting cells of Corynebacterium glutamicum (ATCC 13032) accumulate [14C]lysine by a transport system with a relatively high affinity (10 microMs) and a low maximum velocity (0.15 nmol/min per mg [dry weight]). Uptake of lysine was not inhibited by uncouplers or by ionophores affecting the ion gradients and the energetic state of the cell.
S, Bröer, R, Krämer
openaire   +3 more sources

Stable integration of the Mrx1-roGFP2 biosensor to monitor dynamic changes of the mycothiol redox potential in Corynebacterium glutamicum

Redox Biology, 2019
Mycothiol (MSH) functions as major low molecular weight (LMW) thiol in the industrially important Corynebacterium glutamicum. In this study, we genomically integrated an Mrx1-roGFP2 biosensor in C.
Quach Ngoc Tung, Vu Van Loi, Tobias Busche, Andreas Nerlich, Maren Mieth, Johanna Milse, Jörn Kalinowski, Andreas C. Hocke, Haike Antelmann   +8 more
doaj   +1 more source

Identifying the Growth Modulon of Corynebacterium glutamicum [PDF]

Frontiers in Microbiology, 2019
The growth rate (μ) of industrially relevant microbes, such as Corynebacterium glutamicum, is a fundamental property that indicates its production capacity. Therefore, understanding the mechanism underlying the growth rate is imperative for improving productivity and performance through metabolic engineering.
Thorsten Haas, Michaela Graf, Alexander Nieß, Tobias Busche, Tobias Busche, Jörn Kalinowski, Bastian Blombach, Bastian Blombach, Ralf Takors   +8 more
openaire   +4 more sources

Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors. [PDF]

PLoS ONE, 2017
Corynebacterium glutamicum (C. glutamicum) is a favorable host cell for the production of recombinant proteins, such as important enzymes and pharmaceutical proteins, due to its excellent potential advantages.
Yang Sun, Wenwen Guo, Fen Wang, Chunjun Zhan, Yankun Yang, Xiuxia Liu, Zhonghu Bai   +6 more
doaj   +1 more source

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source

CREAT: A CRISPR‐Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction

Advanced Science, EarlyView.
Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan, Zhe Gao, Yingxuan Qi, Yang Zhang, Xuhui Tian, Pengpeng Zhao, Xu Feng, Qunxin She   +7 more
wiley   +1 more source