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Counter-current chromatography of black tea infusions

Journal of Chromatography A, 1990
Counter-current chromatography using a multilayer coil planet centrifuge, with solvent system ethyl acetate-butanol-water, permits the separation of black tea infusions into fractions which include pure SII and a mixture of SI and SIa thearubigins. Good resolution of several components of the infusion may be achieved in elution times of 1 to 2 h.
B L, Wedzicha, M F, Lo, T J, Donovan
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A novel approach to modelling counter-current chromatography

Journal of Chromatography A, 2010
Literature lists a number of counter-current chromatography (CCC) models that can predict the retention time and to a certain extent the peak width of a solute eluting from a CCC column. The approach described in this paper distinguishes itself from previous reports by relating all model parameters directly to column dimensions and experimental ...
Hacer, Guzlek   +3 more
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Oncolytic virus purification with periodic counter‐current chromatography

Biotechnology and Bioengineering, 2021
Abstract Virus‐based biologicals are one of the most promising biopharmaceuticals of the 21st century medicine and play a significant role in the development of innovative therapeutic, prophylactic, and clinical applications. Oncolytic virus manufacturing scale can range from 5 L in research and development up to 50 L for clinical ...
João P. Mendes   +6 more
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Modeling counter-current chromatography with non-ideal injection

Journal of Chromatography A, 2020
In general counter-current chromatography systems, there are several off-column fittings between injector and column inlet, such as bends, valves, connecting tubes and joints. Due to these off-column fittings, the sample will diffuse in the mobile phase and form an irregular distribution when it flows from the injector to the column inlet.
Haijun, Chen   +7 more
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Controlled-cycle counter-current chromatography

Journal of Chromatography A, 2008
The application of the technique of controlled-cycle operation to counter-current chromatography is suggested and evaluated. The operating cycle as applied to a chromatographic column would consist of two individually timed periods: (1) Flow period: a specified volume of mobile phase is fed into one end of the column, conveyed through it, and ...
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Advances in the separation of gangliosides by counter-current chromatography (CCC)

Journal of Chromatography B, 2021
Gangliosides play critical roles in the development of many progressive diseases. Due to their structural diversity, efficient methods are needed to separate individual gangliosides for studies of their functions, and for use as standards in the analysis of ganglioside mixtures.
Nuanyi, Liang   +3 more
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Recent advances in counter-current chromatography

Journal of Chromatography A, 1991
During the past several years, counter-current chromatography (CCC) technology has been advanced to cover a broad spectrum of applications, from large-scale preparative to analytical-scale separations. These advances include liquid-liquid dual CCC, foam CCC and partition of macromolecules with aqueous-aqueous polymer phase systems.
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Recent Development in Counter-current Chromatography

Chinese Journal of Analytical Chemistry, 2016
Abstract Counter-current chromatography (CCC) is a quick preparative separation technique with immiscible liquid-liquid solvent systems. This paper reviews recent research progress in the instruments improvement of CCC and screening of solvent systems. Moreover, some applications and prospective about chiral resolution by CCC are discussed.
Da-Tong WU, Yuan-Jiang PAN
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Purification of a synthetic myristylated peptide by counter-current chromatography

Journal of Chromatography A, 1991
A preparative purification of myristyl-Gly-Asn-Ile-Phe-Ala-Asn-Leu-Phe-Lys-Gly-Leu-Phe-Gly-Lys-Lys-Glu -NH2 was accomplished using the multi-coil counter-current chromatograph. A partition coefficient was determined in the n-butanol-acetic acid-water (4:1:5) system. Chromatographic runs were made in this system and one modified with ethyl acetate.
M, Knight   +4 more
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Service life of counter-current chromatography coils

Journal of Chromatography A, 2007
A multilayer coil of PTFE tubing, which failed after being used each workday for about 3 years in a type J centrifuge, was examined. Two types of defects were found. One, called crazes, occurs throughout the coil and does not leak initially, but may eventually lead to a short, axially oriented slit.
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