Results 241 to 250 of about 2,861,963 (268)

Molecular cloning of covalently closed circular DNA of bovine leukemia virus

Journal of Virology, 1984
The two species of covalently closed circular DNA molecules of bovine leukemia virus were cloned in the lambda phage vector lambda gtWES X lambda B. Of the nine independent recombinant lambda-bovine leukemia virus clones that were analyzed, three were derived from the small and six were derived from the large circular molecules carrying, respectively ...
S V, Kashmiri, R, Mehdi, J F, Ferrer
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Serum hepatitis B core-related antigen (HBcrAg) correlates with covalently closed circular DNA transcriptional activity in chronic hepatitis B patients

Journal of Hepatology, 2019
Background & Aims: It has been proposed that serum hepatitis B core-related antigen (HBcrAg) reflects intrahepatic covalently closed circular (ccc)DNA levels.
Floriana Facchetti, Barbara Testoni, Alessandro Loglio   +2 more
exaly   +3 more sources

Rapid alkaline preparation for yeast circular covalently closed DNA molecules

Current Genetics, 1985
The alkaline preparation of prokaryotic plasmids (Birnboim and Doly, 1979) has been here adapted to yeast. By simple denaturation and renaturation steps we recovered, from Saccharomyces cerevisiae and Schizosaccharomyces pombe, a population of nucleic acid molecules highly enriched in circular forms. In S.
FILETICI P., JUNAKOVIC N., BALLARIO, Paola   +2 more
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Rapid preparation of covalently closed circular DNA by acridine yellow affinity chromatography

Analytical Biochemistry, 1981
Abstract Covalently closed circular DNA can be isolated rapidly from cell lysates in a two-step process. Hydroxylapatite chromatography to prepurify the plasmid DNA from contaminating protein and RNA is followed by a step gradient elution of covalently closed circular (CCC) plasmid DNA from an acridine yellow affinity column.
W S, Vincent, E S, Goldstein
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Formation of the pool of covalently closed circular viral DNA in hepadnavirus-infected cells

Cell, 1986
Covalently closed circular (CCC) double-stranded DNA believed to be the transcriptional template for duck hepatitis B virus (DHBV) is amplified in aging primary cultures of hepatocytes from congenitally infected ducklings. Analysis of 5-bromodeoxyuridine-labeled heavy/light CCC DNA shows that the relaxed circular DNA synthesized in the cytoplasm by ...
J S, Tuttleman, C, Pourcel, J, Summers
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The facile generation of covalently closed, circular DNAs with defined negative superhelical densities

Analytical Biochemistry, 1982
Abstract A rapid and simple method for generating samples of circular DNAs with defined superhelical densities is presented. A wide range of negative superhelical densities can be produced; namely, from 0 to 0.165 under the conditions employed. This method should facilitate the study of the detailed relationship between superhelical density and ...
C K, Singleton, R D, Wells
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Preparation of covalently closed and open circular DNA molecules of phage λ

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
Abstract Open circles of Escherichia coli phage λ DNA can be efficiently prepared by incubation of the DNA at alkaline pH. Covalently closed circles can be prepared by superinfecting a lysogen with λ phage and pelleting chromosomal DNA; the circles, which may contain bound protein, remain in the supernatant. The advantages of these new procedures are
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Isolation of covalently closed circular DNA of high molecular weight from bacteria

Analytical Biochemistry, 1976
Abstract A simple method is described in detail for the efficient isolation of high molecular weight covalently closed circular DNA (ccc-DNA) from Agrobacterium . Although this method was developed for isolating ccc-DNA of molecular weights greater than 10 8 daltons in Agrobacterium , the technique also proves to be useful in isolating ccc-DNA of ...
T C, Currier, E W, Nester
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Large-scale isolation of covalently closed circular DNA using gel filtration chromatography

Analytical Biochemistry, 1988
The isolation of covalently closed circular (ccc) DNA free of contamination by RNA and other forms of DNA is fundamental to molecular biology. A variety of methods have been explored but CsCl density-gradient centrifugation remains the method most widely used for preparative scale resolution.
G J, Raymond, P K, Bryant, A, Nelson, J D, Johnson   +3 more
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Molecular Aspects of Bleomycin-Promoted Damage of Covalently Closed Circular DNA

1979
An extensive amount of information has been accumulated concerning the extracellular degradation of DNA by bleomycin since its discovery in 1966 by Umezawa et al. (1). It has been established that the drug causes the release of free bases, ruptures the phosphodiester backbone, and damages the deoxyribose moiety of DNA.
Charles W. Haidle, R. Stephen Lloyd, Donald L. Robberson   +2 more
openaire   +1 more source