Results 51 to 60 of about 1,718 (152)
ABSTRACT Photosynthetic light‐harvesting complexes mediate light absorption and energy dissipation. By modulating the photosystems' absorption cross‐section, they affect both photosynthetic activity and non‐photochemical quenching (NPQ). These processes are often studied by spectrally integrated chlorophyll fluorescence, masking their associated ...
Maximiliano Cainzos +5 more
wiley +1 more source
In the chlorophyll (Chl) biosynthesis pathway the formation of protochlorophyllide is catalyzed by Mg-protoporphyrin IX methyl ester (MgPME) cyclase. The Ycf54 protein was recently shown to form a complex with another component of the oxidative cyclase ...
Sarah eHollingshead +12 more
doaj +1 more source
Participation of Glutamate-354 of the CP43 Polypeptide in the Ligation of Manganese and the Binding of Substrate Water in Photosystem II [PDF]
In the current X-ray crystallographic structural models of photosystem II, Glu354 of the CP43 polypeptide is the only amino acid ligand of the oxygen-evolving Mn(4)Ca cluster that is not provided by the D1 polypeptide. To further explore the influence of this structurally unique residue on the properties of the Mn(4)Ca cluster, the CP43-E354Q mutant of
Service, Rachel J +9 more
openaire +3 more sources
Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley +1 more source
Histidine Protonation and the Oxidizing Power of the Oxygen‐Evolving Complex
Protonation of D1‐His337 governs the oxidizing power of the Mn4CaO5 cluster in photosystem II. Its singly protonated form is unstable, resulting in histidine oxidation and disruption of Mn oxidation, whereas the doubly protonated form is stable and upshifts the cluster redox potential, enabling efficient water oxidation.
Keisuke Saito, Hiroshi Ishikita
wiley +1 more source
Recent advances in the structural biology of photosystem II
SUMMARY Photosystem II (PSII) is the membrane protein‐pigment complex responsible for the light‐driven oxidation of water to molecular oxygen, a reaction that enables the aerobic biosphere and powers biological carbon fixation. Over the past two decades, structural biology has transformed our understanding of PSII from low‐resolution outlines of its ...
James W. Murray
wiley +1 more source
将菠菜43kD叶绿素结合蛋白编码基因亚克隆至原核表达载体pET-28a上,构建重组表达质粒pET-28a-psbC转化感受态E.coli BL21(DE3),经IPTG诱导实现了外源基因的可溶性表达.探讨了含组氨酸标签融合蛋白的最佳表达条件(包括表达单克隆、时间、温度对表达的影响).利用Ni2+-Sepharose 4Fast Flow亲合层析纯化出His-apo CP43蛋白.体外重组实验证实His-apo CP43能特异结合叶绿素a ...
刘骥 +4 more
doaj
Stabilization of Chlorophyll a-binding Apoproteins P700, CP47, CP43, D2, and D1 by Chlorophyll a or Zn-pheophytin a [PDF]
Stabilization of chlorophyll a-binding apoproteins P700, CP47, CP43, D2, and D1 against proteolytic degradation has been investigated through in vitro synthesis of chlorophyll a or Zn-pheophytin a in intact etioplasts from barley. Stabilization of the apoproteins was dependent on the concentration of chlorophyll a or Zn-pheophytin a.
L A, Eichacker +3 more
openaire +2 more sources
Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from Plants [PDF]
A method to isolate and purify CP43 and CP47 pigment-protein complexes from Photosystem (PS) II of higher plants is presented. The method has been developed in spinach, but it may also be valid for other plant species, since there is high PSII core complex homology in all plants.
Rafael, Picorel +2 more
openaire +2 more sources
From isolation to insights: mitochondrial complex I in the diatom Phaeodactylum tricornutum
SUMMARY Diatoms are among the most ecologically successful microalgae, contributing significantly to marine primary production and global carbon cycling. Their distinctive metabolic architecture, shaped by a complex evolutionary history involving secondary endosymbiosis, includes a highly compartmentalized cell organization and unique metabolic ...
Federico Berdun +7 more
wiley +1 more source

