Results 61 to 70 of about 2,381 (181)

Synthesis of chlorophyll-binding proteins in a fully-segregated ∆ycf54 strain of the cyanobacterium Synechocystis PCC 6803

Frontiers in Plant Science, 2016
In the chlorophyll (Chl) biosynthesis pathway the formation of protochlorophyllide is catalyzed by Mg-protoporphyrin IX methyl ester (MgPME) cyclase. The Ycf54 protein was recently shown to form a complex with another component of the oxidative cyclase ...
Sarah eHollingshead, Sarah eHollingshead, Jana eKopecna, David R Armstrong, Lenka eBucinska, Philip J Jackson, Philip J Jackson, Guangyu E Chen, Mark J Dickman, Michael P Williamson, Roman eSobotka, Roman eSobotka, Christopher Neil Hunter   +12 more
doaj   +1 more source

Comment on Photosystem II: light‐dependent oscillation of ligand composition at its active site: existence of O6 in S3‐state photosystem II revealed by omit maps

Acta Crystallographica Section D, Volume 82, Issue 6, Page 574-586, June 2026.
We reanalyzed our XFEL data, the results of which showed the insertion of O6 during the S2–S3 transition in water oxidation by photosystem II. These results contradict the interpretation proposed by Wang that there is no insertion of O6 during the S2–S3 transition.Photosystem II (PSII) catalyzes the light‐driven oxidation of water to molecular oxygen ...
Hongjie Li, Michihiro Suga, Jian-Ren Shen   +2 more
wiley   +1 more source

Glutamate-354 of the CP43 polypeptide interacts with the oxygen-evolving Mn4Ca cluster of photosystem II: a preliminary characterization of the Glu354Gln mutant

, 2008
In the recent X-ray crystallographic structural models of photosystem II, Glu354 of the CP43 polypeptide is assigned as a ligand of the O 2-evolving Mn4Ca cluster.
Britt, R David, Melodie A Strickler, Richard J Debus, Hillier, Warwick, Strickler, Melodie A, Hong Jin Hwang, R. David Britt, Burnap, Robert L, Service, Rachel J, Robert L Burnap, Junko Yano, Walker, Lee M, Debus, Richard John, Warwick Hillier, Hwang, Hong Jin, Yano, Junko, Lee M Walker, Rachel J Service   +17 more
core   +1 more source

菠菜43kD叶绿素a结合蛋白编码基因克隆及原核表达条件优化

四川大学学报. 自然科学版, 2015
将菠菜43kD叶绿素结合蛋白编码基因亚克隆至原核表达载体pET-28a上,构建重组表达质粒pET-28a-psbC转化感受态E.coli BL21(DE3),经IPTG诱导实现了外源基因的可溶性表达.探讨了含组氨酸标签融合蛋白的最佳表达条件(包括表达单克隆、时间、温度对表达的影响).利用Ni2+-Sepharose 4Fast Flow亲合层析纯化出His-apo CP43蛋白.体外重组实验证实His-apo CP43能特异结合叶绿素a ...
刘骥, 王豪, 肖清洁, 谢思思, 杜林方   +4 more
doaj  

Reconstructing the origin of oxygenic photosynthesis: do assembly and photoactivation recapitulate evolution?

Frontiers in Plant Science, 2016
Due to the great abundance of genomes and protein structures that today span a broad diversity of organisms, now more than ever before, it is possible to reconstruct the molecular evolution of protein complexes at an incredible level of detail.
Tanai eCardona
doaj   +1 more source

Stabilization of Chlorophyll a-binding Apoproteins P700, CP47, CP43, D2, and D1 by Chlorophyll a or Zn-pheophytin a [PDF]

Journal of Biological Chemistry, 1996
Stabilization of chlorophyll a-binding apoproteins P700, CP47, CP43, D2, and D1 against proteolytic degradation has been investigated through in vitro synthesis of chlorophyll a or Zn-pheophytin a in intact etioplasts from barley. Stabilization of the apoproteins was dependent on the concentration of chlorophyll a or Zn-pheophytin a.
L A, Eichacker, M, Helfrich, W, Rüdiger, B, Müller   +3 more
openaire   +2 more sources

Loss of qE Does Not Necessarily Lead to Photoinhibition: Sustained Non‐Photochemical Quenching in the Absence of PsbS and Zeaxanthin

Plant, Cell &Environment, Volume 49, Issue 6, Page 3405-3425, June 2026.
ABSTRACT Photosynthetic light‐harvesting complexes mediate light absorption and energy dissipation. By modulating the photosystems' absorption cross‐section, they affect both photosynthetic activity and non‐photochemical quenching (NPQ). These processes are often studied by spectrally integrated chlorophyll fluorescence, masking their associated ...
Maximiliano Cainzos, Chen Hu, Maria Dolores Pissolato, Nazeer Fataftah, Sanchali Nanda, Stefan Jansson   +5 more
wiley   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, Volume 600, Issue 10, Page 1536-1548, May 2026.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from Plants [PDF]

, 2010
A method to isolate and purify CP43 and CP47 pigment-protein complexes from Photosystem (PS) II of higher plants is presented. The method has been developed in spinach, but it may also be valid for other plant species, since there is high PSII core complex homology in all plants.
Rafael, Picorel, Miguel, Alfonso, Michael, Seibert   +2 more
openaire   +2 more sources

Histidine Protonation and the Oxidizing Power of the Oxygen‐Evolving Complex

ChemPhotoChem, Volume 10, Issue 5, May 2026.
Protonation of D1‐His337 governs the oxidizing power of the Mn4CaO5 cluster in photosystem II. Its singly protonated form is unstable, resulting in histidine oxidation and disruption of Mn oxidation, whereas the doubly protonated form is stable and upshifts the cluster redox potential, enabling efficient water oxidation.
Keisuke Saito, Hiroshi Ishikita
wiley   +1 more source