Results 81 to 90 of about 5,163,287 (193)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Study on Fatigue Crack Growth Prediction and Machine Learning Correction for Deepwater Risers

Journal of Marine Science and Engineering
Under long-term marine environmental loading, deep-water risers are highly susceptible to fatigue damage, and the accumulation of local damage may lead to global structural failure. In this study, the fatigue damage mechanism and crack growth behavior of
Fucheng Wang, Yong Yang, Baolei Cui, Di Wang   +3 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Smaller is better: nanobodies meet NMR

FEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

The effect of weld residual stresses and their re-distribution with crack growth during fatigue under constant amplitude loading [PDF]

, 2010
In this work the evolution of the residual stresses in a MIG-welded 2024-T3 aluminium alloy M(T) specimen during in situ fatigue crack growth at constant load amplitude has been measured with neutron diffraction.
Lin, J., Liljedahl, C. D. M., Fitzpatrick M, Edwards, L., Edwards, Lyndon, Fitzpatrick, Michael E., Zanellato, O., Fitzpatrick, M. E., Edwards L, Zanellato, Oliver, Liljedahl C, Liljedahl, C. David M., Lin J, Zanellato O, Liljedahl, CDM, Edwards, L, Lin, J, Fitzpatrick, ME, Zanellato, O   +18 more
core   +1 more source

Isothermal and thermomechanical fatigue interaction in fatigue crack propagation behavior of a low-carbon nitrogen-controlled 316 stainless steel

Fracture and Structural Integrity, 2019
In this work, the effect of superimposing of isothermal Low Cycle Fatigue (LCF) loading to the thermomechanical (TMF) fatigue loading on the short crack propagation behavior of low-carbon nitrogen-controlled 316 stainless steel is investigated.
Yasuhiro Yamazaki
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source