Results 111 to 120 of about 403,344 (296)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Discussion on the Anchor Space of Disintegrate Structure Rock Mass Cutting Slope by Using Fracture Mechanics

open access: yesShuitu baochi yanjiu, 2010
Anchor rope spacing is the key parameter on the design of slope anchor.Because the formation of potential failure surface in the cataclastic rock slope is under the control of the inner main crack,fracture mechanics theory is introduced in this paper in ...
LUO Qiang1, LI Liang1, TANG Shu-Ming2
doaj  

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Hexagonal piezoelectric half-space containing a crack

open access: yesApplied Mathematical Modelling, 2002
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
openaire   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

Three-dimensional crack growth in multi-layered architectures for automotive plain bearings

open access: yes, 2009
The assessment of shielding and anti-shielding effects on crack growth in multi-layered architectures is achieved using elasto-plastic finite element models.
Burke-Veliz, A.   +2 more
core   +1 more source

An explainable AI framework for crack width and crack spacing prediction with serviceability-oriented design optimization of reinforced and prestressed concrete members

open access: yesJournal of Engineering and Applied Science
Reliable prediction of cracking behavior is essential for the serviceability assessment of reinforced and prestressed concrete members, where crack development depends on interacting material, geometric, reinforcement, prestressing, and loading ...
Ahmed N. Elbelacy
doaj   +1 more source

Plasma EV Proteomics Identifies ECM Remodeling and Inflammatory Proteins LUM and C7 as Candidate Biomarkers in FSHD

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Facioscapulohumeral muscular dystrophy (FSHD) is one of the most debilitating and common muscular dystrophies. Despite its severity, no approved therapy exists for FSHD patients. However, several therapeutic candidates are currently under development, and some have recently entered clinical trials, marking the need for reliable ...
Mustafa Bilal Bayazit   +11 more
wiley   +1 more source

Digital Cognitive Phenotyping for Differential Diagnosis and Monitoring in Neurological Conditions

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To assess the utility, accessibility, and equivalence to supervised scales of online cognitive assessment in older individuals with cognitive impairment. Methods Patients with Alzheimer's disease (AD, n = 31), idiopathic normal pressure hydrocephalus (iNPH, n = 26), and traumatic brain injury (TBI, n = 23) completed online cognitive ...
Martina Del Giovane   +10 more
wiley   +1 more source

Flexural Behavior of Alkali-Activated Ultra-High-Performance Geopolymer Concrete Beams

open access: yesBuildings
Ultra-high-performance geopolymer concrete (UHPGC) emerges as a sustainable and cost-effective alternative to Portland cement-based UHPC, offering similar mechanical properties while significantly reducing carbon footprint and energy consumption ...
Jie Su, Jiandong Tan, Kai Li, Zhi Fang
doaj   +1 more source

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