Results 111 to 120 of about 403,344 (296)
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Anchor rope spacing is the key parameter on the design of slope anchor.Because the formation of potential failure surface in the cataclastic rock slope is under the control of the inner main crack,fracture mechanics theory is introduced in this paper in ...
LUO Qiang1, LI Liang1, TANG Shu-Ming2
doaj
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Hexagonal piezoelectric half-space containing a crack
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openaire +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Three-dimensional crack growth in multi-layered architectures for automotive plain bearings
The assessment of shielding and anti-shielding effects on crack growth in multi-layered architectures is achieved using elasto-plastic finite element models.
Burke-Veliz, A. +2 more
core +1 more source
Reliable prediction of cracking behavior is essential for the serviceability assessment of reinforced and prestressed concrete members, where crack development depends on interacting material, geometric, reinforcement, prestressing, and loading ...
Ahmed N. Elbelacy
doaj +1 more source
ABSTRACT Objective Facioscapulohumeral muscular dystrophy (FSHD) is one of the most debilitating and common muscular dystrophies. Despite its severity, no approved therapy exists for FSHD patients. However, several therapeutic candidates are currently under development, and some have recently entered clinical trials, marking the need for reliable ...
Mustafa Bilal Bayazit +11 more
wiley +1 more source
Digital Cognitive Phenotyping for Differential Diagnosis and Monitoring in Neurological Conditions
ABSTRACT Objective To assess the utility, accessibility, and equivalence to supervised scales of online cognitive assessment in older individuals with cognitive impairment. Methods Patients with Alzheimer's disease (AD, n = 31), idiopathic normal pressure hydrocephalus (iNPH, n = 26), and traumatic brain injury (TBI, n = 23) completed online cognitive ...
Martina Del Giovane +10 more
wiley +1 more source
Flexural Behavior of Alkali-Activated Ultra-High-Performance Geopolymer Concrete Beams
Ultra-high-performance geopolymer concrete (UHPGC) emerges as a sustainable and cost-effective alternative to Portland cement-based UHPC, offering similar mechanical properties while significantly reducing carbon footprint and energy consumption ...
Jie Su, Jiandong Tan, Kai Li, Zhi Fang
doaj +1 more source

