Results 221 to 230 of about 389,860 (253)

Evolution of CRISPR-associated Endonucleases as Inferred from Resurrected Proteins

2022
Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9 protein is an effector that plays a major role in a prokaryotic adaptive immune system, by which invading DNA can be targeted and cut for inactivation.
Borja Alonso-Lerma, Ylenia Jabalera, Matias Morin, Almudena Fernandez, Sara Samperio, Ane Quesada, Antonio Reifs, Sergio Fernández-Peñalver, Yolanda Benitez, Lucia Soletto, Jose A Gavira, Adrian Diaz, Wim Vranken, Benjamin P. Kleinstiver, Avencia Sanchez-Mejias, Marc Güell, Francisco JM Mojica, Miguel A Moreno-Pelayo, Lluis Montoliu, Raul Perez-Jimenez   +19 more
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Molecular Details of DNA Integration by CRISPR-Associated Proteins During Adaptation in Bacteria and Archaea

2022
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in bacteria and archaea, where immunological memory is retained in the CRISPR locus as short pieces of the intruding nucleic acid, termed spacers. The adaptation to new infections occurs through the integration of
Tamara, Flusche, Rakhi, Rajan
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Adaptive immunity of type VI CRISPR-Cas systems associated with reverse transcriptase–Cas1 fusion proteins [PDF]

Nucleic Acids Research
Cas13-containing type VI CRISPR-Cas systems specifically target RNA; however, the mechanism of spacer acquisition remains unclear. We have previously reported the association of reverse transcriptase–Cas1 (RT–Cas1) fusion proteins with certain types of ...
Maria Dolores Molina-Sánchez, FRANCISCO Martínez-Abarca, Vicenta Millán   +2 more
exaly   +2 more sources

Current Updates of CRISPR/Cas System and Anti-CRISPR Proteins: Innovative Applications to Improve the Genome Editing Strategies

International Journal of Nanomedicine
Khaled S Allemailem,1 Ahmad Almatroudi,1 Faris Alrumaihi,1 Arwa Essa Alradhi,2 Abdulrahman Theyab,3,4 Mohammad Algahtani,3 Mohmmed Othman Alhawas,5 Gasim Dobie,6 Amira A Moawad,7,8 Arshad Husain Rahmani,1 Amjad Ali Khan9 1Department of Medical ...
Theyab A, Khan AA, Algahtani M, Rahmani AH, Alhawas MO, Almatroudi A, Alrumaihi F, Alradhi AE, Moawad AA, Dobie G, Allemailem KS   +10 more
exaly   +2 more sources

X‐ray crystal structure of a CRISPR‐associated protein, Cse2, from Thermus thermophilus HB8

Proteins: Structure, Function, and Bioinformatics, 2008
X-ray crystal structure of a CRISPRassociated protein, Cse2, from Thermus thermophilus HB8 Yoshihiro Agari, Shigeyuki Yokoyama, Seiki Kuramitsu, and Akeo Shinkai* 1 Photon Science Research Division, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan 2 Systems and Structural Biology Center, Yokohama Institute, RIKEN, 1-7 ...
Yoshihiro, Agari, Shigeyuki, Yokoyama, Seiki, Kuramitsu, Akeo, Shinkai   +3 more
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Crystal structure and nucleic acid‐binding activity of the CRISPR‐associated protein Csx1 of Pyrococcus furiosus

Proteins: Structure, Function, and Bioinformatics, 2012
AbstractIn many prokaryotic organisms, chromosomal loci known as clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR‐associated (CAS) genes comprise an acquired immune defense system against invading phages and plasmids. Although many different Cas protein families have been identified, the exact biochemical functions of most
Kim, YK Kim, Young Kwan, Kim, YG Kim, Yeon-Gil, Oh, BH Oh, Byung-Ha   +2 more
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A strategy for the isolation and refolding of aggregation-prone proteins: application to two CRISPR-associated Cas12m effectors

Molecular Genetics, Microbiology and Virology (Russian)
Research Objective. To develop refolding conditions for recombinant proteins prone to aggregation and inclusion body formation during expression in Escherichia coli, using two homologs of the CRISPR effector Cas12m as an example. Materials and Methods. The Cas12m genes were amplified and cloned into expression vectors with an N-terminal His-tag.
A. V. Vlaskina, D. E. Petrenko, Yu. K. Agapova, A. A. Kuzminkova, M. A. Evteeva, M. V. Patrushev   +5 more
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Defining the allosteric role of RNA in the CRISPR-associated Cas13a protein activation

Biophysical Journal, 2023
Souvik Sinha, Adrian Moises Molina Vergas, Pablo R. Arantes, Amun C. Patel, Mitchell O’Connell, Giulia Palermo   +5 more
openaire   +1 more source

Cysteine‐independent CRISPR‐Associated Protein Labeling for Presentation and Co‐delivery of Molecules Toward Genetic and Epigenetic Regulations

ChemBioChem
AbstractLabeling of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) associated proteins (Cas) remains an immense challenge for their genome engineering applications. To date, cysteine‐mediated bioconjugation is the most efficient strategy for labeling Cas proteins.
Sadiya Tanga, Arpita Hota, Arkadeep Karmakar, Paramita Banerjee, Basudeb Maji   +4 more
openaire   +2 more sources

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins

Nature Reviews Molecular Cell Biology, 2021
Ning Jia, Dinshaw J Patel, Patel Dinshaw J   +2 more
exaly