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CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges [PDF]
HeliyonCRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 and CRISPR/Cas3 utilize different mechanisms to detect or ...
Jian Zhou +3 more
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Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations
eLife, 2017 CRISPR-Cas provides a barrier to horizontal gene transfer in prokaryotes. It was previously observed that functional CRISPR-Cas systems are absent from multidrug-resistant (MDR) Enterococcus faecalis, which only possess an orphan CRISPR locus, termed ...
Karthik Hullahalli +2 more
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Functional Coupling and Evolutionary Relationships Between Toxin–Antitoxin Systems and CRISPR-Cas Systems [PDF]
ToxinsBacteria encode a broad range of survival and defence systems, including CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas systems, restriction-modification systems, and toxin–antitoxin (TA) systems, which are involved in bacterial ...
Yibo Meng, Jiyun Chen, Liang Liu
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CRISPR-Cas is beneficial in plasmid competition, but limited by competitor toxin-antitoxin activity when horizontally transferred. [PDF]
PLoS BiologyBacteria can encode dozens of different immune systems that protect them from infection by mobile genetic elements (MGEs). MGEs themselves may also carry immune systems, such as CRISPR-Cas, to target competitor MGEs. It is unclear when this is favored by
David Sünderhauf +6 more
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A tailored phosphorothioate coordinator enables CRISPR/Cas in-situ amplification. [PDF]
Nucleic Acids ResYang T +11 more
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CRISPR/Cas Technology for the Diagnosis of Animal Infectious Diseases [PDF]
MicroorganismsIncreasingly complex epidemics of animal infectious diseases have emerged as a major risk to livestock production and human health. However, current detection methods for animal infectious diseases suffer from shortcomings such as insufficient ...
Shuling Meng +5 more
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CASPredict: a web service for identifying Cas proteins [PDF]
PeerJ, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated (Cas) proteins constitute the CRISPR-Cas systems, which play a key role in prokaryote adaptive immune system against invasive foreign elements.
Shanshan Yang, Jian Huang, Bifang He
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Phylogenetic Distribution of CRISPR-Cas Systems in Staphylococcus lugdunensis
Microbiology Spectrum, 2021 Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) genes (CRISPR-Cas) are present in many bacterial genomes with functions beyond adaptive immunity.
Cheng-Yen Kao +4 more
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Biosensors, 2022 Pathogen-specific point-of-care (PoC) diagnostic tests have become an important need in the fight against infectious diseases and epidemics in recent years. PoC diagnostic tests are designed with the following parameters in mind: rapidity, accuracy, sensitivity, specificity, and ease of use.
Nazente Atçeken +3 more
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Microbiology Spectrum, 2023 CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-Cas; however, in the human pathogen Staphylococcus aureus, CRISPR-Cas loci are less common and often studied ...
Kasper Mikkelsen +11 more
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