Results 91 to 100 of about 1,216,536 (293)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

CRISPR-Cas-Based Biomonitoring for Marine Environments: Toward CRISPR RNA Design Optimization Via Deep Learning

, 2023
Almost all of Earth's oceans are now impacted by multiple anthropogenic stressors, including the spread of nonindigenous species, harmful algal blooms, and pathogens. Early detection is critical to manage these stressors effectively and to protect marine
Durán-Vinet, Benjamín, Kardailsky, Anya, Susanna A. Wood, Neil J. Gemmell, Xavier Pochon, Maryam Moarefian, Jeunen, Gert-Jan, Anya Kardailsky, Aran, Kiana, Araya-Castro, Karla, Jo-Ann L. Stanton, Tzu-Chiao Chao, Anastasija Zaiko, Michelle Scriver, Deependra K. Ban, Benjamín Durán-Vinet, Ban, Deependra K, Wood, Susanna A, Pochon, Xavier, Gemmell, Neil J, Wood, Susanna, Moarefian, Maryam, Stanton, Jo-Ann L, Karla Araya-Castro, Kiana Aran, Gert-Jan Jeunen, Chao, Tzu-Chiao, Zaiko, Anastasija, Scriver, Michelle   +28 more
core   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

CRISPR-Cas Systems Impact Pseudomonas aeruginosa Genome Structure

, 2016
Pseudomonas aeruginosa is both an antibiotic-resistant opportunistic pathogen and an important model of type I clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR-Cas) systems.
Kristofer Miller (2862299), Kyle C. Cady (2862296), Todd C. Peterson (2427787), Gregory Hannum (255439), Toby H. Richardson (251546), Leah B. Soriaga (2862302), E. Magda Barbu (254713), Daniel Brami (126239), Srividya Akella (2862293), Bolyn Hubby (94177), Martina Felderman (2862305), Ariel Schwartz (521629), Matthew C. LaFave (594263), Alex van Belkum (38419), Mark C. Enright (255200)   +14 more
core   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

CRISPR: An overview of CRISPR-Cas systems and their diagnostic methods for pathogen detection

Life Sciences, Medicine and Biomedicine
Early and rapid diagnosis of pathogens is crucial for preventing and controlling epidemic diseases. While traditional microbial detection methods serve their purpose, they often have limitations such as low sensitivity and specificity.
Pui Yuei Lee, Christina Shook Cheng Chong, Mee Hoong See, Lee Lee Lai, Paul Michels, Choon Jiat Lee, Rukumani Devi Velayuthan, Yoke Kiet Doon, Yong Hui Tan, Yin Yin Lau, Sze Wan Poong, Phaik Eem Lim, Crystale Siew Ying Lim   +12 more
doaj   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system

Heliyon
CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications.
Hui Cheng, Haoyue Deng, Dongdao Ma, Mengyuan Gao, Zhihan Zhou, Heng Li, Shejuan Liu, Tieshan Teng   +7 more
doaj   +1 more source