Results 81 to 90 of about 80,955 (297)

Epidemiological and evolutionary consequences of different types of CRISPR-Cas systems.

PLoS Computational Biology, 2022
Bacteria have adaptive immunity against viruses (phages) in the form of CRISPR-Cas immune systems. Currently, 6 types of CRISPR-Cas systems are known and the molecular study of three of these has revealed important molecular differences. It is unknown if
Hélène Chabas, Viktor Müller, Sebastian Bonhoeffer, Roland R Regoes   +3 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

CRISPR-Cas: biology, mechanisms and relevance [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2016
Prokaryotes have evolved several defence mechanisms to protect themselves from viral predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) display a prokaryotic adaptive immune system that memorizes previous infections by integrating short sequences of invading genomes—termed spacers—into the ...
Frank Hille, Emmanuelle Charpentier
openaire   +4 more sources

Clades of huge phages from across Earth's ecosystems. [PDF]

, 2020
Bacteriophages typically have small genomes1 and depend on their bacterial hosts for replication2. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of ...
Al-Shayeb, Basem, Amano, Yuki, Amundson, Ronald, Anantharaman, Karthik, Banfield, Jillian F, Borton, Mikayla A, Bouma-Gregson, Keith, Brooks, Brandon, Castelle, Cindy J, Cate, Jamie HD, Chen, Lin-Xing, Devoto, Audra, Doudna, Jennifer A, Farag, Ibrahim F, Finstad, Kari, Goltsman, Daniela SA, Harrison, Sue, He, Christine, Jaffe, Alexander L, Kantor, Rose, Keren, Ray, Lane, Katherine R, Lavy, Adi, Lehours, Anne-Catherine, Lei, Shufei, Li, Wen-Jun, Matheus-Carnevali, Paula, Morowitz, Michael, Munk, Patrick, Méheust, Raphaël, Nelson, Tara C, Olm, Matthew R, Power, Mary E, Probst, Alexander J, Relman, David A, Sachdeva, Rohan, Santini, Joanne M, Sharrar, Allison, Sun, Christine, Thomas, Alex, Tringe, Susannah G, Ward, Fred, Warren, Lesley, Wrighton, Kelly, Zhou, Jinglie   +44 more
core   +2 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

CRISPR-Cas influences the acquisition of antibiotic resistance in Klebsiella pneumoniae.

PLoS ONE, 2019
In the US Carbapenem resistance in Klebsiella pneumoniae (Kp) is primarily attributed to the presence of the genes blaKPC-2 and blaKPC-3, which are transmitted via plasmids.
Natalie A Mackow, Juntao Shen, Mutayyaba Adnan, Aisha S Khan, Bettina C Fries, Elizabeth Diago-Navarro   +5 more
doaj   +1 more source

CRISPR-Cas systems in multicellular cyanobacteria [PDF]

RNA Biology, 2018
Novel CRISPR-Cas systems possess substantial potential for genome editing and manipulation of gene expression. The types and numbers of CRISPR-Cas systems vary substantially between different organisms. Some filamentous cyanobacteria harbor > 40 different putative CRISPR repeat-spacer cassettes, while the number of cas gene instances is much lower ...
Shengwei Hou, Manuel Brenes-Álvarez, Viktoria Reimann, Omer S. Alkhnbashi, Rolf Backofen, Alicia M. Muro-Pastor, Wolfgang R. Hess   +6 more
openaire   +4 more sources

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

Biology Direct, 2011
Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to ...
Wolf Yuri I, Aravind L, Makarova Kira S, Koonin Eugene V   +3 more
doaj   +1 more source