Results 171 to 180 of about 1,216,536 (293)

Editorial: creative CRISPR-Cas: RNA-guided functions in defence and beyond. [PDF]

Microlife
Hess WR, Marchfelder A, Randau L.
europepmc   +1 more source

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Detection of CRISPR-Cas-induced mutations in Daphnia. [PDF]

G3 (Bethesda)
Neupane S, Pfrender ME, Wang L, Xu S.
europepmc   +1 more source

CRISPR-Cas Antiviral Strategies

Virus Research, 2018
Jin, Dong-Yan, Berkhout, Ben
openaire   +2 more sources

Nucleoside‐Modified mRNA Encoding Alpha‐Galactosidase A Ameliorates Fabry Disease Phenotypes in Human IPSC‐Derived Cardiomyocytes

Advanced Science, EarlyView.
Human iPSC‐derived Fabry cardiomyocytes exhibited broad transcriptional dysregulation, apoptosis, mitochondrial dysfunction, impaired reactive oxygen species handling, altered contractility, and abnormal calcium transient decay, potentially mediated by phospholamban hyperphosphorylation.
Malte Juchem, Lea Oehlsen, Sedef Ersoy, Jia Li Ye, Natalie Weber, Elisa Mohr, Wilson Agyapong, Junqing Liu, Maximilian Fuchs, Ke Xiao, Christopher Jahn, Reto Eggenschwiler, Tobias Cantz, Julia Beimdiek, Falk F. R. Buettner, Theresia Kraft, Jan Hegermann, Ivonne J. Knorr, Lisa Ernst, Linda Feldbrügge, Resa Puffert, Kevin Schmidt, Christian Bär, Jeannine Hoepfner, Thomas Thum   +24 more
wiley   +1 more source

Exploring the emerging role of CRISPR-Cas systems in probiotic development. [PDF]

Eng Microbiol
Hussain A, Mojgani N, Siddiqui MF, Khan MI, Ali SA.   +4 more
europepmc   +1 more source

USP30‐Mediated Deubiquitination of PEX5 Suppresses Pexophagy to Drive Tubular Injury in Diabetic Kidney Disease

Advanced Science, EarlyView.
USP30 accumulation in diabetic renal tubular epithelial cells deubiquitinates PEX5, suppresses pexophagy, and drives dysfunctional peroxisome accumulation and tubular injury. USP30 depletion restores PEX5 ubiquitination and pexophagy, improves peroxisomal function, and alleviates tubular damage, highlighting USP30 as a therapeutic target in diabetic ...
Jia Li, Chaoyang Hua, Guangpu Li, Furong Zhai, Jingjing Ren, Shaokang Pan, Qi Feng, Jianguo Wen, Dongwei Liu, Zhangsuo Liu, Jiayu Duan   +10 more
wiley   +1 more source

Evaluating high-fidelity CRISPR-Cas nucleases in nucleosomal contexts using a quantitative framework. [PDF]

Front Genome Ed
Handelmann CR, Skeens E, Lisi GP, Buck MJ.   +3 more
europepmc   +1 more source

Asymmetric Evolution of Antennal Cell Types Underlies a Derived Ammonia‐Sensing Logic for Ecological Adaptation in Bactrocera dorsalis

Advanced Science, EarlyView.
Comparative antennal single‐nucleus transcriptomics reveals cell‐type‐dependent transcriptomic divergence in Bactrocera dorsalis, with similar structural cells but divergent sensory neurons. This neuronal diversification is associated with a distinct ammonia‐sensing pathway, linking female attraction to bird‐dropping‐associated cues with nutritional ...
Wei Liu, Shuai Zhang, Jie Zhang, Zhen Tian, Yuanbo Xue, Ling Yang, Jun Xu, Guirong Wang   +7 more
wiley   +1 more source

10 years of CRISPR/CAS genomic engineering in Yarrowia lipolytica. [PDF]

Bioprocess Biosyst Eng
Dias RG, Freitas FPM, Barbosa SL, Assis JVMG, Entringer TL, Fonseca JSC, Romanizio ME, Pimentel BBZ, Campos-Galvão MEM, Vieira NM, Fietto LG, Zsögön A, da Silveira WB.   +12 more
europepmc   +1 more source