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CRISPR-Cas Systems in Prokaryotes [PDF]
Polish Journal of Microbiology, 2015 Prokaryotic organisms possess numerous strategies that enable survival in hostile conditions. Among others, these conditions include the invasion of foreign nucleic acids such as bacteriophages and plasmids. The clustered regularly interspaced palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) system provides the majority of bacteria and ...
Burmistrz, Michał, Pyrć, Krzysztof
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CRISPR-Cas Controls Cryptic Prophages [PDF]
International Journal of Molecular Sciences, 2021 The bacterial archetypal adaptive immune system, CRISPR-Cas, is thought to be repressed in the best-studied bacterium, Escherichia coli K-12. We show here that the E. coli CRISPR-Cas system is active and serves to inhibit its nine defective (i.e., cryptic) prophages.
Sooyeon Song +6 more
openaire +2 more sources
Indian Journal of Community Medicine, 2023 Background: The CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems are the short DNA sequences and RNA-dependent nuclease involved in the adaptive immunity in bacteria and archaea. The type of CRISPR-
Anand Babu Kannadasan +2 more
doaj +1 more source
Characterization of CRISPR-Cas Systems in Serratia marcescens Isolated from Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae) [PDF]
, 2019 The CRISPR-Cas adaptive immune system has been attracting increasing scientific interest for biological functions and biotechnological applications. Data on the Serratia marcescens system are scarce.
Calia, Carla +7 more
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Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
Application of different types of CRISPR/Cas-based systems in bacteria
Microbial Cell Factories, 2020 As important genome editing tools, CRISPR/Cas systems, especially those based on type II Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria.
Zhenquan Liu +4 more
doaj +1 more source
A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]
, 2015 Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G. +7 more
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High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
Nature Biotechnology, 2013 Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell–based reporter assay to characterize off-target cleavage
Yanfang Fu +7 more
semanticscholar +1 more source
Advances in Engineering the Fly Genome with the CRISPR-Cas System. [PDF]
, 2018 Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate ...
Bier, Ethan +3 more
core +2 more sources
Research advances on pathogenic nucleic acid detection technology based on CRIPSR/Cas system
浙江大学学报. 农业与生命科学版, 2023 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas) system, an ancient bacterial and archaeal immune system, has rapidly developed into a popular gene-editing tool, which largely promotes the ...
LI Hongzhao +4 more
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