Results 111 to 120 of about 216,867 (346)
In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus [PDF]
, 2013 Clustered regularly interspaced short palindromic repeats (CRISPR)-encoded immunity in Type I systems relies on the Cascade (CRISPR-associated complex for antiviral defence) ribonucleoprotein complex, which triggers foreign DNA degradation by an ...
Giedrius Gasiunas +8 more
core +2 more sources
CRISPR-Cas systems for diagnosing infectious diseases
Methods, 2022 Infectious diseases are a global health problem affecting billions of people. Developing rapid and sensitive diagnostic tools is key for successful patient management and curbing disease spread. Currently available diagnostics are very specific and sensitive but time-consuming and require expensive laboratory settings and well-trained personnel; thus ...
Kostyusheva, Anastasiya +6 more
openaire +2 more sources
Molecular Oncology, EarlyView.Tumors contain diverse cellular states whose behavior is shaped by context‐dependent gene coordination. By comparing gene–gene relationships across biological contexts, we identify adaptive transcriptional modules that reorganize into distinct vulnerability axes.
Brian Nelson +9 more
wiley +1 more source
Nonviral Delivery of CRISPR/Cas Systems in mRNA Format
Advanced NanoBiomed Research, 2022 The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas) genome editing system provides a powerful toolbox for genetic engineering.
Zhen Liu, Zhenghua Li, Bin Li
doaj +1 more source
EspB and HtpG interact with the type III-A CRISPR/Cas system of Mycobacterium tuberculosis
Frontiers in Molecular Biosciences, 2023 Introduction:Mycobacterium tuberculosis (MTB) has a type III-A clustered regularly interspaced short palindromic repeat/CRISPR-associated protein (CRISPR/Cas) system consisting of a Csm1-5 and CRISPR RNA (crRNA) complex involved in the defense against ...
Mingmin Shi +15 more
doaj +1 more source
CRISPR-Cas Systems Reduced to a Minimum [PDF]
Molecular Cell, 2019 In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.
Almendros, Cristóbal +2 more
openaire +3 more sources
RIPK4 function interferes with melanoma cell adhesion and metastasis
Molecular Oncology, EarlyView.RIPK4 promotes melanoma growth and spread. RIPK4 levels increase as skin lesions progress to melanoma. CRISPR/Cas9‐mediated deletion of RIPK4 causes melanoma cells to form less compact spheroids, reduces their migratory and invasive abilities and limits tumour growth and dissemination in mouse models.
Norbert Wronski +9 more
wiley +1 more source
Regulation of Clostridiodes difficile CRISPR-Cas system by biofilm-associated factors and glucose
Инфекция и иммунитетClostridioides difficile is a spore-forming enteropathogenic anaerobic bacterium and one of the most common opportunistic human pathogens. Its pathogenicity relies on the production of toxins, sporulation, biofilm formation, and the ability to withstand ...
Anna S. Maikova +2 more
doaj +1 more source
Efficient genome editing in plants using a CRISPR/Cas system
Cell Research, 2013 .
Zhengyan Feng +10 more
semanticscholar +1 more source
CRISPR-Cas System: An Approach With Potentials for COVID-19 Diagnosis and Therapeutics
Frontiers in Cellular and Infection Microbiology, 2020 COVID-19, the human coronavirus disease caused by SARS-CoV-2, was reported for the first time in Wuhan, China in late 2019. COVID-19 has no preventive vaccine or proven standard pharmacological treatment, and consequently, the outbreak swiftly became a ...
Prashant Kumar +11 more
semanticscholar +1 more source