Results 91 to 100 of about 75,041 (301)

CRISPR-Cas Systems Reduced to a Minimum [PDF]

Molecular Cell, 2019
In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.
Almendros, Cristóbal, Kieper, Sebastian N., Brouns, Stan J.J.   +2 more
openaire   +3 more sources

RIPK4 function interferes with melanoma cell adhesion and metastasis

Molecular Oncology, EarlyView.
RIPK4 promotes melanoma growth and spread. RIPK4 levels increase as skin lesions progress to melanoma. CRISPR/Cas9‐mediated deletion of RIPK4 causes melanoma cells to form less compact spheroids, reduces their migratory and invasive abilities and limits tumour growth and dissemination in mouse models.
Norbert Wronski, Sławomir Lasota, Ewelina Madej, Anna A. Brożyna, Małgorzata Szczygieł, Agnieszka Harazin‐Lechowska, Jan Czerbniak, Janusz Rys, Jaroslaw Czyz, Agnieszka Wolnicka‐Glubisz   +9 more
wiley   +1 more source

Regulation of Clostridiodes difficile CRISPR-Cas system by biofilm-associated factors and glucose

Инфекция и иммунитет
Clostridioides difficile is a spore-forming enteropathogenic anaerobic bacterium and one of the most common opportunistic human pathogens. Its pathogenicity relies on the production of toxins, sporulation, biofilm formation, and the ability to withstand ...
Anna S. Maikova, D. E. Polev, M. A. Khodorkovskii   +2 more
doaj   +1 more source

Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]

, 2018
The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan, Liu, Fenyong, Sheng, Jingxue, Trang, Phong   +3 more
core   +2 more sources

Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]

, 2020
The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes, Jonckheere, Wim, Livadaras, Ioannis, Riga, Maria, Van Leeuwen, Thomas, Vontas, John   +5 more
core   +1 more source

Application of CRISPR Cas Systems for Biosensing

Biosensors, 2023
The essential properties of a biosensor are its sensitivity and selectivity to detect, monitor and quantify the biomarker(s) for the interests of medicine [...]
Chung Chiun Liu, Yifan Dai
openaire   +3 more sources

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Advances in biotechnology: genomics and genome editing [PDF]

, 2017
Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve.
Beccari, Tommaso, Dundar, Munis, Gartland, Jill S., Gartland, Kevan M.A., Magni, Mariapia Viola   +4 more
core   +1 more source

CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding [PDF]

, 2020
Open Access Journal; Published online: 31 Jan 2019Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids.
Britt, A., Muiruri, S.K., Ntui, V.O., Ron, M., Tripathi, J.N., Tripathi, L.   +5 more
core   +1 more source

CRISPR-Cas systems in enterococci

Brazilian Journal of Microbiology
Enterococci are members of the microbiota of humans and other animals. They can also be found in the environment, associated with food, healthcare infections, and hospital settings. Due to their wide distribution, they are inserted in the One Health context.
Amanda Seabra Cabral, Fernanda de Freitas Lacerda, Vitor Luis Macena Leite, Filipe Martire de Miranda, Amanda Beiral da Silva, Bárbara Araújo dos Santos, Jailton Lobo da Costa Lima, Lúcia Martins Teixeira, Felipe Piedade Gonçalves Neves   +8 more
openaire   +2 more sources