Results 111 to 120 of about 18,275,652 (330)

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

The stress of carrying CRISPR-Cas

Virulence
Streptococcus anginosus (S. anginosus) is a commensal that can cause severe invasive bacterial infections. A considerable percentage of S. anginosus strains harbour CRISPR-Cas systems, which apart from being a bacterial immunity system, can play an ...
Dorina Haider, Richard Bauer, Aline Grempels, Rebecca Roscher, Cagil Ceren Aslan, Stefanie Mauerer, Barbara Spellerberg   +6 more
doaj   +1 more source

BIOINFORMATIC SEARCH OF CRISPR/CAS SYSTEM STRUCTURES IN GENOME OF PCT281 PLASMID OF BACILLUS THURINGIENSIS SUBSP. CHINENSIS STRAIN CT-43

Acta Biomedica Scientifica, 2018
Background. CRISPR/Cas systems loci are one of the functionally important patterns in bacterial genome which perform the role of “adaptive immune defense” from foreign nucleic acids.
N. A. Arefyeva, Yu. P. Dzhioev, A. Yu. Borisenko, V. I. Chemerilova, O. F. Vyatchina, O. A. Sekerina, L. A. Stepanenko, Yu. A. Markova, G. V. Yurinova, V. P. Salovarova, A. A. Pristavka, V. A. Kuzminova, O. N. Reva, V. I. Zlobin   +13 more
doaj   +1 more source

CRISPR-Cas Systems Impact Pseudomonas aeruginosa Genome Structure

, 2016
Pseudomonas aeruginosa is both an antibiotic-resistant opportunistic pathogen and an important model of type I clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR-Cas) systems.
Kristofer Miller (2862299), Kyle C. Cady (2862296), Todd C. Peterson (2427787), Gregory Hannum (255439), Toby H. Richardson (251546), Leah B. Soriaga (2862302), E. Magda Barbu (254713), Daniel Brami (126239), Srividya Akella (2862293), Bolyn Hubby (94177), Martina Felderman (2862305), Ariel Schwartz (521629), Matthew C. LaFave (594263), Alex van Belkum (38419), Mark C. Enright (255200)   +14 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

CRISPR-mediated antiviral defence in prokaryotes [PDF]

, 2010
cum laude graduation (with ...
Jore, M.M.
core  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

CRISPR-Cas system

, 2016
Bacteria can receive genetic material from other bacteria or invading bacteriophages primarily through horizontal gene transfer. These genetic exchanges can result in genome rearrangement and the acquisition of novel traits that assist cells with stresses and adverse environmental conditions.
Hakim, Joseph A., Koo, Hyunmin, van Elsas, Jan D., Trevors, Jack T., Bej, Asim K.   +4 more
openaire   +1 more source