Results 111 to 120 of about 75,041 (301)

CRISPR-Cas systems are present predominantly on mobile genetic elements in Vibrio species

BMC Genomics, 2019
Background Bacteria are prey for many viruses that hijack the bacterial cell in order to propagate, which can result in bacterial cell lysis and death.
Nathan D. McDonald, Abish Regmi, Daniel P. Morreale, Joseph D. Borowski, E. Fidelma Boyd   +4 more
doaj   +1 more source

Optimization of CRISPR–Cas system for clinical cancer therapy

Bioengineering & Translational Medicine, 2023
Cancer is a genetic disease caused by alterations in genome and epigenome and is one of the leading causes for death worldwide. The exploration of disease development and therapeutic strategies at the genetic level have become the key to the treatment of
Xiang Meng, Tian‐gang Wu, Qiu‐yue Lou, Kai‐yuan Niu, Lei Jiang, Qing‐zhong Xiao, Tao Xu, Lei Zhang   +7 more
doaj   +1 more source

Applications of CRISPR–Cas systems in neuroscience [PDF]

, 2016
Genome-editing tools, and in particular those based on CRISPR-Cas (clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein) systems, are accelerating the pace of biological research and enabling targeted genetic ...
A Bolotin, A Fire, A Geling, A Plessis, A Xiao, A Zeisel, AJ Wood, AM Geurts, AN Shah, AW Chan, AY Cheung, B Chen, B Pardo, B Zetsche, B Zetsche, C Burger, C Pak, C Straub, D Kim, D Maddalo, D St Johnston, DB Cox, DC van Gent, E Deltcheva, E Sasaki, EA Brenowitz, EB Lewis, EE Patton, EM Jorgensen, F Liang, F Soldner, F Zhang, FA Ran, FA Ran, FD Urnov, Feng Zhang, G Gasiunas, G Murlidharan, GP Flowers, H Akil, H Liu, H Wang, H Xia, HB Kordasiewicz, I Harel, I Jeon, IB Hilton, ID Carbery, IE Clark, J Boch, J Fishman-Lobell, J Fishman-Lobell, J Morton, J Peters, JA Harris, JA Zuris, JC Belmonte, JC Miller, JD Hommel, JD Sander, JD Sander, JD Sweatt, JE Garneau, JL Bessereau, JM Taymans, KJ Beumer, KR Thomas, KS Makarova, KY Kim, L Cong, L Cooley, L Swiech, LA Gilbert, LE Dow, LE Jao, LR Polstein, LS Qi, M Bibikova, M Bibikova, M Christian, M Garriga-Canut, M Jinek, M Maresca, M Zuckermann, Matthias Heidenreich, MC Marchetto, MJ Moscou, N Gaiano, N Rudin, N Wittenburg, NA Kearns, P Essletzbichler, P Mali, P Mali, P Qiu, P Rouet, P Rouet, PD Hsu, R Barrangou, RA Smith, RB Blasco, RD Johnson, RJ Platt, RR Beerli, S Incontro, S Konermann, S Konermann, S Oddo, SD Ryan, SH Yang, SM Elbashir, SQ Tsai, T Katsuyama, T Yagi, TO Auer, VM Bedell, Y Doyon, Y Fu, Y Niu, YG Kim, Z Shen   +120 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Effective genome editing and identification of a regiospecific gallic acid 4-O-glycosyltransferase in pomegranate (Punica granatum L.). [PDF]

, 2019
Pomegranate (Punica granatum L.) trees are woody perennials that bear colorful and nutritious fruits rich in phenolic metabolites, e.g., hydrolyzable tannins (HTs) and flavonoids.
Chang, Lijing, Tian, Li, Wu, Sheng
core   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. [PDF]

, 2017
Imaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for imaging of chromatin within live cells. However, these methods are
Adli, Mazhar, Bandaria, Jigar, Darzacq, Xavier, Kuscu, Cem, Mir, Mustafa, Parlak, Mahmut, Qin, Peiwu, Singh, Ritambhara, Szlachta, Karol, Yildiz, Ahmet   +9 more
core   +1 more source

The CRISPR-Cas system

, 2020
Derived from and inspired by the adaptive immune system of bacteria, CRISPR has gone from basic biology knowledge to a revolutionizing biotechnological tool, applicable in many research areas such as medicine, industry and agriculture. The full mechanism of CRISPR-Cas9 was first published in 2012 and various CRISPR-Cas systems have already passed the ...
Stens, Cassandra, Enoksson, Isabella, Berggren, Sara   +2 more
openaire   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

CRISPR–cas loci profiling of Cronobacter sakazakii pathovars [PDF]

, 2016
Aim: Cronobacter sakazakii sequence types 1, 4, 8 and 12 are associated with outbreaks of neonatal meningitis and necrotizing enterocolitis infections. However clonality results in strains which are indistinguishable using conventional methods.
Forsythe, SJ, Ogrodzki, P
core   +1 more source