Results 91 to 100 of about 717,978 (310)

The Reverse Transcriptases Associated with CRISPR-Cas Systems

Scientific Reports, 2017
AbstractCRISPR (clustered regularly interspaced short palindromic repeats) and associated proteins (Cas) act as adaptive immune systems in bacteria and archaea. Some CRISPR-Cas systems have been found to be associated with putative reverse transcriptases (RT), and an RT-Cas1 fusion associated with a type III-B system has been shown to acquire RNA ...
Toro, Nicolás, Martínez-Abarca, Francisco, González-Delgado, Alejandro   +2 more
openaire   +5 more sources

Exploring DNA Functionalization Techniques for Silver Nanoparticles

Advanced Materials Interfaces, EarlyView.
This study evaluates four functionalization methods—salt‐aging, pH‐assisted, freezing‐directed, and microwave‐assisted—for attaching DNA strands to 20 nm silver nanoparticles (AgNPs). The resulting DNA‐AgNP conjugates are characterized and colloidal stability and DNA loading are assessed.
Sebastian Freko, Marta Nikić, Lennart J.K. Weiß, Bernhard Wolfrum   +3 more
wiley   +1 more source

Diversity, classification and evolution of CRISPR-Cas systems [PDF]

Current Opinion in Microbiology, 2017
The bacterial and archaeal CRISPR-Cas systems of adaptive immunity show remarkable diversity of protein composition, effector complex structure, genome locus architecture and mechanisms of adaptation, pre-CRISPR (cr)RNA processing and interference. The CRISPR-Cas systems belong to two classes, with multi-subunit effector complexes in Class 1 and single-
Koonin, Eugene V, Makarova, Kira S, Zhang, Feng   +2 more
openaire   +5 more sources

Evaporation‐Enhanced Redox Cycling for Rapid Detection of Attomolar SARS‐CoV‐2 Virions Using Nanolithography‐Free Electrochemical Devices

Advanced Materials Technologies, EarlyView.
This study presents microfabricated electrochemical sensors for detecting ultralow viral particles counts through evaporation‐enhanced redox cycling (E2RC). To achieve sub‐micrometer gap in interdigitated microelectrodes, a scalable, nanolithography‐free fabrication method is developed.
Pouya Soltan Khamsi, Vinay Kammarchedu, Aida Ebrahimi   +2 more
wiley   +1 more source

BIOINFORMATIC SEARCH OF CRISPR/CAS SYSTEM STRUCTURES IN GENOME OF PCT281 PLASMID OF BACILLUS THURINGIENSIS SUBSP. CHINENSIS STRAIN CT-43

Acta Biomedica Scientifica, 2018
Background. CRISPR/Cas systems loci are one of the functionally important patterns in bacterial genome which perform the role of “adaptive immune defense” from foreign nucleic acids.
N. A. Arefyeva, Yu. P. Dzhioev, A. Yu. Borisenko, V. I. Chemerilova, O. F. Vyatchina, O. A. Sekerina, L. A. Stepanenko, Yu. A. Markova, G. V. Yurinova, V. P. Salovarova, A. A. Pristavka, V. A. Kuzminova, O. N. Reva, V. I. Zlobin   +13 more
doaj   +1 more source

Development and Application of CRISPR/Cas in Microbial Biotechnology

Frontiers in Bioengineering and Biotechnology, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding, Wentao Ding, Yang Zhang, Shuobo Shi   +3 more
doaj   +1 more source

Complex Systems + Systems Engineering = Complex Systems Engineeri [PDF]

arXiv, 2006
One may define a complex system as a system in which phenomena emerge as a consequence of multiscale interaction among the system's components and their environments. The field of Complex Systems is the study of such systems--usually naturally occurring, either bio-logical or social.
arxiv  

Control of gene expression by CRISPR-Cas systems [PDF]

F1000Prime Reports, 2013
Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage
Luciano A. Marraffini, David Bikard
openaire   +3 more sources

Autologous Peripheral Vγ9Vδ2 T Cell Synergizes with αβ T Cell Through Antigen Presentation and BTN3A1 Blockade in Immunotherapy of Cervical Cancer

Advanced Science, EarlyView.
This study provides proof‐of‐concept evidence showing that through direct killing, antigen presentation, and competitively binding to BTN3A1, Vγ9Vδ2 T cells amplified from cervical cancer (CC) patients can synergize with αβ T cells to exert anti‐CC function both in vitro and in vivo.
Min Wu, Jian Liu, Liting Liu, Yifan Yang, Hong Liu, Long Yu, Haihong Zeng, Shuo Yuan, Ruiyi Xu, Hangyu Liu, Han Jiang, Shen Qu, Liming Wang, Ying Chen, Jingyu Wang, Yuwei Zhang, Shan He, Ling Feng, Junyan Han, Wanjiang Zeng, Hui Wang, Yafei Huang   +21 more
wiley   +1 more source

Systems of quotients of Lie triple systems [PDF]

Communications in Algebra, 42(2014)(8), 3339-3349, 2013
In this paper, we introduce the notion of system of quotients of Lie triple systems and investigate some properties which can be lifted from a Lie triple system to its systems of quotients. We relate the notion of Lie triple system of Martindale-like quotients with respect to a filter of ideals and the notion of system of quotients, and prove that the ...
arxiv