Results 111 to 120 of about 58,663 (324)
The Reverse Transcriptases Associated with CRISPR-Cas Systems
Scientific Reports, 2017 AbstractCRISPR (clustered regularly interspaced short palindromic repeats) and associated proteins (Cas) act as adaptive immune systems in bacteria and archaea. Some CRISPR-Cas systems have been found to be associated with putative reverse transcriptases (RT), and an RT-Cas1 fusion associated with a type III-B system has been shown to acquire RNA ...
Toro, Nicolás +2 more
openaire +5 more sources
Diversity, classification and evolution of CRISPR-Cas systems [PDF]
Current Opinion in Microbiology, 2017 The bacterial and archaeal CRISPR-Cas systems of adaptive immunity show remarkable diversity of protein composition, effector complex structure, genome locus architecture and mechanisms of adaptation, pre-CRISPR (cr)RNA processing and interference. The CRISPR-Cas systems belong to two classes, with multi-subunit effector complexes in Class 1 and single-
Koonin, Eugene V +2 more
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Advances in Engineering the Fly Genome with the CRISPR-Cas System. [PDF]
, 2018 Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate ...
Bier, Ethan +3 more
core +2 more sources
Acta Biomedica Scientifica, 2018 Background. CRISPR/Cas systems loci are one of the functionally important patterns in bacterial genome which perform the role of “adaptive immune defense” from foreign nucleic acids.
N. A. Arefyeva +13 more
doaj +1 more source
Lipid Nanoparticle‐Mediated CRISPR‐Cas13a Delivery for the Control of Bacterial Infection
Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.New formulations of lipid nanoparticles (LNPs) that can deliver nucleic acids to Gram‐negative bacteria are proposed to combat bacterial infection. The delivery of nucleic acids by LNPs is aided by LNP‐helpers which weaken the bacterial outer membrane. LNPs encapsulating the Cas13a/gRNA expression vector achieve an antibacterial effect in both in vivo ...
Bookun Kim +9 more
wiley +1 more source
Control of gene expression by CRISPR-Cas systems [PDF]
F1000Prime Reports, 2013 Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage
Luciano A. Marraffini, David Bikard
openaire +3 more sources
Glucocorticoids Alter Bone Microvascular Barrier via MAPK/Connexin43 Mechanisms
Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.Osteoporosis induced by Glucocorticoids (GCs) is a common complication of long‐term GC use in patients with inflammatory and autoimmune conditions, often leading to an increased fracture risk. A 3D bicellular endo‐osteo microfluidic platform is developed to explore pathogenic mechanisms underlying GCs‐induced osteoporosis, highlighting a novel MAPK ...
Eun‐Jin Lee +10 more
wiley +1 more source
Recent Applications of Mesoporous Silica Nanoparticles in Gene Therapy
Advanced Healthcare Materials, EarlyView.The review summarizes the synthesis of mesoporous silica nanoparticles (MSNs) with modifiable surface properties, functionalization strategies, mechanism of therapeutic payload release, and current applications in gene therapy, focusing on their capabilities in the targeted delivery of therapeutic nucleic acids, CRISPR‐Cas systems, and other genetic ...
Tamanna Binte Huq +4 more
wiley +1 more source
A use/disuse paradigm for CRISPR-Cas systems [PDF]
Biology & Philosophy, 2019 In his insightful review, Eugene V. Koonin discusses various aspects of CRISPR-Cas systems with a strong focus on their qualities as "adaptive (acquired) immune systems"(Koonin 2018, 3). The CRISPR-Cas system is most famous for its application as a gene-editing tool. Koonin provides a deeper insight into its biological function in bacteria, which is to
openaire +4 more sources
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source