Results 71 to 80 of about 277,742 (368)
A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila
Fly, 2020 Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out.
Kevin G. Nyberg +5 more
doaj +1 more source
pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens [PDF]
arXiv, 2023 We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR library sequencing coverage across all time points and samples.
arxiv
BioTechniques, 2017 Sarah Webb explores how researchers are using CRISPR/Cas9 to solve agricultural problems.
openaire +3 more sources
Stochastic variation in the FOXM1 transcription program mediates replication stress tolerance
Molecular Oncology, EarlyView.Cellular heterogeneity is a major cause of drug resistance in cancer. Segeren et al. used single‐cell transcriptomics to investigate gene expression events that correlate with sensitivity to the DNA‐damaging drugs gemcitabine and prexasertib. They show that dampened expression of transcription factor FOXM1 and its target genes protected cells against ...
Hendrika A. Segeren +4 more
wiley +1 more source
Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A
mLifeIn Sulfolobales cells, transcription of the Ups (UV‐inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili‐mediated cell aggregation and ...
Pengju Wu +6 more
doaj +1 more source
The physicist's guide to one of biotechnology's hottest new topics: CRISPR-Cas [PDF]
, 2017 Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells. A heritable, molecular memory is generated of phage, plasmids, or other mobile genetic elements that attempt to attack the cell.
arxiv +1 more source
Molecular Oncology, EarlyView.TOMM20 increases cancer aggressiveness by maintaining a reduced state with increased NADH and NADPH levels, oxidative phosphorylation (OXPHOS), and apoptosis resistance while reducing reactive oxygen species (ROS) levels. Conversely, CRISPR‐Cas9 knockdown of TOMM20 alters these cancer‐aggressive traits.
Ranakul Islam +9 more
wiley +1 more source
mLifeSaccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems.
Pengpeng Zhao +6 more
doaj +1 more source
SpCas9-expression by tumor cells can cause T cell-dependent tumor rejection in immunocompetent mice
OncoImmunology, 2019 The CRISPR/Cas9 system has recently emerged as a highly efficient modality in genetic engineering and has been widely considered for various therapeutic applications. However, since the effector protein, SpCas9, has a bacterial origin, its immunogenicity
Reham Ajina +6 more
doaj +1 more source
Plants, 2020 Phospholipids belong to a significant class of lipids and comprise ~10% of total lipids in rice grains. Lysophospholipid (LPL) is derived from the hydrolysis of phospholipids and plays an important role in rice grain quality.
Muhammad Saad Shoaib Khan +4 more
doaj +1 more source