Results 51 to 60 of about 363,164 (302)

Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley

Plants, 2020
Inositol trisphosphate 5/6 kinases (ITPK) constitute a small group of enzymes participating in the sequential phosphorylation of inositol phosphate to inositol hexakisphosphate (IP6), which is a major storage form of phosphate in cereal grains.
Tomáš Vlčko, Ludmila Ohnoutková
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Specificity Assessment of CRISPR Genome Editing of Oncogenic EGFR Point Mutation with Single-Base Differences

Molecules, 2019
In CRISPR genome editing, CRISPR proteins form ribonucleoprotein complexes with guide RNAs to bind and cleave the target DNAs with complete sequence complementarity. CRISPR genome editing has a high potential for use in precision gene therapy for various
Taegeun Bae, Hanseop Kim, Jeong Hee Kim, Yong Jun Kim, Seung Hwan Lee, Byung-Joo Ham, Junho K. Hur   +6 more
doaj   +1 more source

CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs) in macrophages with an NF-κB reporter. [PDF]

, 2017
The innate immune system protects against infections by initiating an inducible inflammatory response. NF-κB is one of the critical transcription factors controlling this complex response, but some aspects of its regulation remain unclear.
Carpenter, Susan, Covarrubias, Sergio, Fong, Nicholas, Hanley, Nicole, Katzman, Sol, McManus, Michael T, Robinson, Elektra K, Shapleigh, Barbara, Vollmers, Apple   +8 more
core   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila

Fly, 2020
Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out.
Kevin G. Nyberg, Joseph Q. Nguyen, Yong-Jae Kwon, Shelby Blythe, Greg J. Beitel, Richard Carthew   +5 more
doaj   +1 more source

CRISPR/Cas9-induced (CTG⋅CAG)n repeat instability in the myotonic dystrophy type 1 locus: implications for therapeutic genome editing [PDF]

, 2017
Myotonic dystrophy type 1 (DM1) is caused by (CTG⋅CAG)n-repeat expansion within the DMPK gene and thought to be mediated by a toxic RNA gain of function.
André, Laurène M., Cumming, Sarah A., Furling, Denis, Gourdon, Geneviève, Monckton, Darren G., Mouly, Vincent, van Agtmaal, Ellen L., van den Broek, Walther J.A.A., van Kessel, Ingeborg D.G., Wansink, Derick G., Wieringa, Bé, Willemse, Marieke   +11 more
core   +4 more sources

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Cran1, member of a new class of OLD family ATPases, functions in cell cycle progression in an archaeon

EMBO Reports
Overcoming lysogenization defect (OLD) proteins are diverse ATPase-nucleases functioning in antiphage defense in bacteria. However, the role of these proteins in archaea is currently unknown.
Yunfeng Yang, Shikuan Liang, Junfeng Liu, Xiaofei Fu, Pengju Wu, Haodun Li, Jinfeng Ni, Qunxin She, Mart Krupovic, Yulong Shen   +9 more
doaj   +1 more source

Crispr strategies for stem cell engineering: a new frontier in musculoskeletal regeneration

European Cells & Materials, 2023
The costs and incidence of musculoskeletal injuries are rapidly increasing due to rising population age, higher prevalence of risk factors, and lack of effective long-term treatments.
JP Graham, LC Werba, I Federico, T Gonzalez-Fernandez   +3 more
doaj   +1 more source