Results 51 to 60 of about 315,823 (196)

Anti‐CRISPRs: The natural inhibitors for CRISPR‐Cas systems [PDF]

Animal Models and Experimental Medicine, 2019
AbstractCRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been developed into a series of powerful gene‐editing tools. As the natural inhibitors of CRISPR‐Cas systems, anti‐CRISPRs (Acrs) can be
Fei Zhang, Guoxu Song, Yong Tian
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An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales

Communications Biology
Archaea of the order Sulfolobales possess eukaryotic-like cell cycle. The chromatin organization in these archaea is supposed to rely on nucleoid-associated proteins (NAPs).
Qi Gan, Haodun Li, Qihong Huang, Yunfeng Yang, Fei Sun, Xu Feng, Jinfeng Ni, Zhenfeng Zhang, Qunxin She, Yulong Shen   +9 more
doaj   +1 more source

Genetically engineered birds; pre-CRISPR and CRISPR era

Biology of Reproduction, 2021
Abstract Generating biopharmaceuticals in genetically engineered bioreactors continues to reign supreme. Hence, genetically engineered birds have attracted considerable attention from the biopharmaceutical industry. Fairly recent genome engineering methods have made genome manipulation an easy and affordable task.
Nima, Dehdilani, Sara, Yousefi Taemeh, Lena, Goshayeshi, Hesam, Dehghani   +3 more
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Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

mLife
Saccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems.
Pengpeng Zhao, Xiaonan Bi, Xiaoning Wang, Xu Feng, Yulong Shen, Guanhua Yuan, Qunxin She   +6 more
doaj   +1 more source

Crispr strategies for stem cell engineering: a new frontier in musculoskeletal regeneration

European Cells & Materials, 2023
The costs and incidence of musculoskeletal injuries are rapidly increasing due to rising population age, higher prevalence of risk factors, and lack of effective long-term treatments.
JP Graham, LC Werba, I Federico, T Gonzalez-Fernandez   +3 more
doaj   +1 more source

Modulation of Hachiman defence by a type II toxin-antitoxin system via balancing trade-off between the fitness cost and antiphage activity

Engineering Microbiology
Hachiman systems provide innate antiphage immunity across prokaryotic domains. The system encodes a HamA nuclease and a HamB helicase both of which exhibit great diversity in sequence.
Xuhui Tian, Ruyi Zheng, Xin Li, Suping Jiang, Fang Wang, Yulong Shen, Qunxin She   +6 more
doaj   +1 more source

CRISPR-based assay reveals SARS-CoV-2 RNA dynamic changes and redistribution patterns in non-human primate model

Emerging Microbes and Infections, 2022
Mounting evidence indicates that SARS-CoV-2 can infect multiple systemic tissues, but few studies have evaluated SARS-CoV-2 RNA dynamics in multiple specimen types due to their reduced accessibility and diminished performance of RT-qPCR with non ...
Zhen Huang, Lili Zhang, Christopher J. Lyon, Bo Ning, Brady M. Youngquist, Alex Niu, Brandon J. Beddingfield, Nicholas J. Maness, Nakhle S. Saba, Chen-Zhong Li, Chad J. Roy, Tony Y. Hu   +11 more
doaj   +1 more source

CRISPR-DO for genome-wide CRISPR design and optimization [PDF]

Bioinformatics, 2016
Abstract Motivation: Despite the growing popularity in using CRISPR/Cas9 technology for genome editing and gene knockout, its performance still relies on well-designed single guide RNAs (sgRNA). In this study, we propose a web application for the Design and Optimization (CRISPR-DO) of guide sequences that target both coding and non ...
Jian Ma, Johannes Köster, Qian Qin, Shengen Hu, Wei Li 0035, Chenhao Chen, Qingyi Cao, Jinzeng Wang, Shenglin Mei, Qi Liu, Han Xu, Xiaole Shirley Liu   +11 more
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CRISPR-Powered DNA Computing and Digital Display

, 2021
Clustered regularly interspaced short palindromic repeats (CRISPR) technology has unique specificity for recognizing and cleaving target DNA complementary to the CRISPR guide sequence. Here, we report on a CRISPR-powered DNA computing and digital display
Jiongyu Zhang, Changchun Liu, Jiongyu Zhang (11613518), Changchun Liu (312471)   +3 more
core   +1 more source

Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope

Nature Communications
TnpBs encoded by the IS200/IS605 family transposon are among the most abundant prokaryotic proteins from which type V CRISPR-Cas nucleases may have evolved.
Xu Feng, Ruyi Xu, Jianglan Liao, Jingyu Zhao, Baochang Zhang, Xiaoxiao Xu, Pengpeng Zhao, Xiaoning Wang, Jianyun Yao, Pengxia Wang, Xiaoxue Wang, Wenyuan Han, Qunxin She   +12 more
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