Results 61 to 70 of about 315,823 (196)

NGS quantification of f-CRISPR

, 2021
Quantification of f-CRISPR editing of WT and oRiboT ribosomes in E ...
Farren J. Isaacs (1323855), Felix Radford (11718530)   +1 more
core   +1 more source

uPAR Knockout Results in a Deep Glycolytic and OXPHOS Reprogramming in Melanoma and Colon Carcinoma Cell Lines

Cells, 2020
Urokinase Plasminogen Activator (uPA) Receptor (uPAR) is a well-known GPI-anchored three-domain membrane protein with pro-tumor roles largely shown in all the malignant tumors where it is over-expressed.
Alessio Biagioni, Anna Laurenzana, Anastasia Chillà, Mario Del Rosso, Elena Andreucci, Martina Poteti, Daniele Bani, Daniele Guasti, Gabriella Fibbi, Francesca Margheri   +9 more
doaj   +1 more source

CRISPR-C: circularization of genes and chromosome by CRISPR in human cells [PDF]

Nucleic Acids Research, 2018
Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-
Henrik Devitt Møller, Lin Lin, Xi Xiang, Trine Skov Petersen, Jinrong Huang, Luhan Yang, Eigil Kjeldsen, Uffe Birk Jensen, Xiuqing Zhang, Xin Liu, Xun Xu, Jian Wang, Huanming Yang, George M Church, Lars Bolund, Birgitte Regenberg, Yonglun Luo   +16 more
openaire   +4 more sources

Engineered microRNA scaffolds for potent gene silencing in vivo

Scientific Reports
RNA interference (RNAi) is emerging as a powerful strategy for therapeutic targeting of “undruggable” targets. However, efficacy of currently used siRNA-based therapies is often hindered by transient effects and limited modeling possibilities. Artificial
Giuseppe Militello, Alyssa Greig, Chongfeng Bi, Ana Vasileva, Maria I. Zavodszky, Shih-Ching Lo, Edward Guilmette, Pete Clarner, Bin Liu, Guruharsha Bhat, Junghae Suh, Lukas Dow, Johannes Zuber, Christof Fellmann, Prem K. Premsrirut   +14 more
doaj   +1 more source

CRISPR-dependent endogenous gene regulation is required for virulence in piscine Streptococcus agalactiae

, 2021
The clustered regularly interspaced palindromic repeats (CRISPR)-Cas (CRISPR-associated) system is a prokaryotic defence against invading mobile genetic elements, such as bacteriophages or exogenous plasmids.
Ke Ma (260231), Yongjie Liu (3166032), Guangjin Liu (216593), Hao Huang (111923), Chengping Lu (167772), Qing Cao (417020), Meng Nie (6894863), Mingguo Jiang (6932633), Yuhao Dong (2180608)   +8 more
core   +1 more source

Author Correction: Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins

Scientific Reports, 2021
Jongjin Park, Jiyoung Yoon, Daekee Kwon, Mi-Jung Han, Sunmee Choi, Slki Park, Junghyuk Lee, Kiwook Lee, Jaehwan Lee, Seunghee Lee, Kyung-Sun Kang, Sunghwa Choe   +11 more
doaj   +1 more source

Whole Genome Analysis of Tibetan Kefir-Derived Lactiplantibacillus Plantarum 12-3 Elucidates Its Genomic Architecture, Antimicrobial and Drug Resistance, Potential Probiotic Functionality and Safety

Frontiers in Bioscience-Landmark
Background: Lactiplantibacillus plantarum 12-3 holds great promise as a probiotic bacterial strain, yet its full potential remains untapped. This study aimed to better understand this potential therapeutic strain by exploring its genomic landscape ...
Tariq Aziz, Muhammad Naveed, Muhammad Aqib Shabbir, Abid Sarwar, Ayaz Ali Khan, Ammarah Hasnain, Taqweem Ul Haq, Zhennai Yang, Abdellah Zinedine, João Miguel Rocha, Metab Alharbi   +10 more
doaj   +1 more source

CRISPR/Cas-based customization of pooled CRISPR libraries

PLOS ONE, 2018
Pooled CRISPR libraries are widely used in high-throughput screening to study various biological processes. Various pooled CRISPR libraries have been shared for CRISPR screens and useful tools have been developed to construct researcher's own libraries, however, many researchers are struggling to create their own pooled CRISPR libraries: it is a time ...
Jiyeon Kweon, Da-eun Kim, An-Hee Jang, Yongsub Kim   +3 more
openaire   +4 more sources

CRISPR-Cas Systems Impact Pseudomonas aeruginosa Genome Structure

, 2016
Pseudomonas aeruginosa is both an antibiotic-resistant opportunistic pathogen and an important model of type I clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR-Cas) systems.
Kristofer Miller (2862299), Kyle C. Cady (2862296), Todd C. Peterson (2427787), Gregory Hannum (255439), Toby H. Richardson (251546), Leah B. Soriaga (2862302), E. Magda Barbu (254713), Daniel Brami (126239), Srividya Akella (2862293), Bolyn Hubby (94177), Martina Felderman (2862305), Ariel Schwartz (521629), Matthew C. LaFave (594263), Alex van Belkum (38419), Mark C. Enright (255200)   +14 more
core   +1 more source

Rapid one-tube RPA-coupled CRISPR/Cas12a-based RID-MyC assay for the diagnosis of fungal keratitis

Indian Journal of Ophthalmology
Purpose: This study introduces and evaluates the single-tube rapid identification of mycoses using CRISPR (ST-RID-MyC) assay. This novel diagnostic tool combines recombinase polymerase amplification (RPA) with CRISPR/Cas12a for the rapid and precise ...
Hanith R Deivarajan, Kanmani Senthilkumar, Hari V Sekar, Vignesh Elamurugan, Jaishree Pandian, Anitha Venugopal, Dharmalingam Kuppamuthu, Lalitha Prajna, Venkatesh N Prajna, Siddharth Narendran   +9 more
doaj   +1 more source