Results 41 to 50 of about 315,823 (196)

Putting the Spotlight on CRISPR [PDF]

BioTechniques, 2019
Rounding up the results from our recent spotlight on CRISPR – are we still just at the beginning for our CRISPR journey?
openaire   +5 more sources

Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley

Plants, 2020
Inositol trisphosphate 5/6 kinases (ITPK) constitute a small group of enzymes participating in the sequential phosphorylation of inositol phosphate to inositol hexakisphosphate (IP6), which is a major storage form of phosphate in cereal grains.
Tomáš Vlčko, Ludmila Ohnoutková
doaj   +1 more source

Specificity Assessment of CRISPR Genome Editing of Oncogenic EGFR Point Mutation with Single-Base Differences

Molecules, 2019
In CRISPR genome editing, CRISPR proteins form ribonucleoprotein complexes with guide RNAs to bind and cleave the target DNAs with complete sequence complementarity. CRISPR genome editing has a high potential for use in precision gene therapy for various
Taegeun Bae, Hanseop Kim, Jeong Hee Kim, Yong Jun Kim, Seung Hwan Lee, Byung-Joo Ham, Junho K. Hur   +6 more
doaj   +1 more source

The influence of the copy number of invader on the fate of bacterial host cells in the antiviral defense by CRISPR-Cas10 DNases

Engineering Microbiology, 2023
Type III CRISPR-Cas10 systems employ multiple immune activities to defend their hosts against invasion from mobile genetic elements (MGEs), including DNase and cyclic oligoadenylates (cOA) synthesis both of which are hosted by the type-specific protein ...
Zhenxiao Yu, Jianan Xu, Yan Zhang, Qunxin She   +3 more
doaj   +1 more source

Cran1, member of a new class of OLD family ATPases, functions in cell cycle progression in an archaeon

EMBO Reports
Overcoming lysogenization defect (OLD) proteins are diverse ATPase-nucleases functioning in antiphage defense in bacteria. However, the role of these proteins in archaea is currently unknown.
Yunfeng Yang, Shikuan Liang, Junfeng Liu, Xiaofei Fu, Pengju Wu, Haodun Li, Jinfeng Ni, Qunxin She, Mart Krupovic, Yulong Shen   +9 more
doaj   +1 more source

A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila

Fly, 2020
Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out.
Kevin G. Nyberg, Joseph Q. Nguyen, Yong-Jae Kwon, Shelby Blythe, Greg J. Beitel, Richard Carthew   +5 more
doaj   +1 more source

CREAT: A CRISPR‐Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction

Advanced Science
The construction of minimal‐genome microbes offers an ideal platform for understanding fundamental biological processes and synthetic biology, yet the research is hindered by incomplete lists of essential genes in microbes and by multiple rounds of ...
Guanhua Yuan, Zhe Gao, Yingxuan Qi, Yang Zhang, Xuhui Tian, Pengpeng Zhao, Xu Feng, Qunxin She   +7 more
doaj   +1 more source

Safety and efficacy of allogeneic CD19-directed CAR-T therapy CTX110 in relapsed/refractory B-cell non-Hodgkin lymphoma

Blood Advances
: This multicenter, single-arm, phase 1/2 study evaluated the safety and efficacy of CD19-directed allogeneic chimeric antigen receptor (CAR) immunotherapy CTX110 in adult patients with relapsed/refractory (R/R) B-cell non-Hodgkin lymphoma (NHL ...
Joseph P. McGuirk, Armin Ghobadi, Carlos R. Bachier, Paul J. Shaughnessy, Farrukh Awan, Joseph E. Maakaron, Michael R. Bishop, P. Joy Ho, Constantine S. Tam, Michael Dickinson, Justin M. Darrah, Edmund K. Waller, Hemant S. Murthy, Ainsley Ross, Leah M. Williams, Chenyi Pan, William N. Stevens, Laura Serwer, Thao Nguyen, Jonathan Terrett, Demetrios Kalaitzidis, Anna Ma, Huansheng Xu, Mark Benton, Annie Weaver, Sarah Beaussant Cohen, Richard T. Maziarz, Chan Cheah   +27 more
doaj   +1 more source

SpCas9-expression by tumor cells can cause T cell-dependent tumor rejection in immunocompetent mice

OncoImmunology, 2019
The CRISPR/Cas9 system has recently emerged as a highly efficient modality in genetic engineering and has been widely considered for various therapeutic applications. However, since the effector protein, SpCas9, has a bacterial origin, its immunogenicity
Reham Ajina, Danielle Zamalin, Annie Zuo, Maha Moussa, Marta Catalfamo, Sandra A. Jablonski, Louis M. Weiner   +6 more
doaj   +1 more source

Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A

mLife
In Sulfolobales cells, transcription of the Ups (UV‐inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili‐mediated cell aggregation and ...
Pengju Wu, Mengqi Zhang, Yanlu Kou, Shikuan Liang, Jinfeng Ni, Qihong Huang, Yulong Shen   +6 more
doaj   +1 more source