Results 31 to 40 of about 315,823 (196)

Desarrollo de nuevos diseños vegetales: Mejora genética vs transformación genética y edición génica

Magna Scientia UCEVA, 2021
El objetivo de esta reflexión es realizar un estudio comparativo sobre la eficacia, eficiencia técnica y costo de las tecnologías más importantes para el desarrollo de nuevos diseños vegetales, incluyendo la mejora genética, la transformación genética y ...
Pedro Martínez Gómez
doaj   +1 more source

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition [PDF]

, 2015
This work was supported by a grant from the Biotechnology and Biological Sciences Research Council (REF: BB/M000400/1 to MFW).The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements.
Bolt, Edward, White, Malcolm F., White, Malcolm F, Anna Sophie Brinkmann, Malcolm F White, Clare Rollie, Edward L Bolt, Brinkmann, Anna Sophie, Rollie, Clare, Rollie, Clare Jane Catherine, Schneider, Stefanie, Stefanie Schneider, Bolt, Edward L., White, Malcolm F; id_orcid   +13 more
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CRISPR-Cas-Based Biomonitoring for Marine Environments: Toward CRISPR RNA Design Optimization Via Deep Learning

, 2023
Almost all of Earth's oceans are now impacted by multiple anthropogenic stressors, including the spread of nonindigenous species, harmful algal blooms, and pathogens. Early detection is critical to manage these stressors effectively and to protect marine
Durán-Vinet, Benjamín, Kardailsky, Anya, Susanna A. Wood, Neil J. Gemmell, Xavier Pochon, Maryam Moarefian, Jeunen, Gert-Jan, Anya Kardailsky, Aran, Kiana, Araya-Castro, Karla, Jo-Ann L. Stanton, Tzu-Chiao Chao, Anastasija Zaiko, Michelle Scriver, Deependra K. Ban, Benjamín Durán-Vinet, Ban, Deependra K, Wood, Susanna A, Pochon, Xavier, Gemmell, Neil J, Wood, Susanna, Moarefian, Maryam, Stanton, Jo-Ann L, Karla Araya-Castro, Kiana Aran, Gert-Jan Jeunen, Chao, Tzu-Chiao, Zaiko, Anastasija, Scriver, Michelle, Gemmell, Neil J., Ban, Deependra K., Stanton, Jo-Ann L., Wood, Susanna A.   +32 more
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Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9 [PDF]

, 2015
CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high
Rojas-Fernandez, Alejandro, Hay, Ronald T., Macartney, Thomas, Herhaus, Lina, Sapkota, Gopal P., Lachaud, Christophe   +5 more
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Self‐Cloning CRISPR [PDF]

Current Protocols in Stem Cell Biology, 2016
AbstractCRISPR/Cas9‐gene editing has emerged as a revolutionary technology to easily modify specific genomic loci by designing complementary sgRNA sequences and introducing these into cells along with Cas9. Self‐cloning CRISPR/Cas9 (scCRISPR) uses a self‐cleaving palindromic sgRNA plasmid (sgPal) that recombines with short PCR‐amplified site‐specific ...
Arbab, M., Sherwood, R. I.
openaire   +2 more sources

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems [PDF]

, 2016
CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of transcription-dependent DNA targeting ...
Zhang, Jing, White, Malcolm Frederick, Tello, Agnes, Liu, Huanting, Graham, Shirley, White, Malcolm F.; id_orcid, Iu, Huanting   +6 more
core   +1 more source

CRISPR-bibliometrics on Wikipedia.

, 2023
A) The number of references in the CRISPR article’s reference section since it opened until December 2021. B) “CRISPR”s SciScore (shown until December 2021).
Ariane Augustoni (16979419), Olha Guley (16979413), Jean-Marc Sevin (16979416), Rona Aviram (779413), Leo Blondel (6329555), Louise Jouveshomme (16979425), Ariel Linder (16979428), Matthieu Collet (16979422), Roy Amit (5439728), Omer Benjakob (16979410)   +9 more
core   +1 more source

The current advances of CRISPR/Cas-based systems for the detection of COVID-19

Journal of Current Biomedical Reports, 2021
The novel coronavirus disease 2019 (COVID-19) still poses a serious threat to every human on the planet nearly 1.5 years after its beginning. Unfortunately, the current diagnostic methods, although highly sensitive and specific, still suffer from many ...
Sasan Ghaffari, Hanif Kazerooni, Amir Salehi-Najafabadi   +2 more
doaj   +1 more source

A human rights analysis of clustered regularly interspaced short palindromic repeats germline-editing for disease prevention

Journal of Public Health and Primary Care, 2020
Our purpose is to discuss ethical concerns with disease prevention applications of CRISPR germline editing using a human rights approach. If applied towards furthering health, these tools may aid to prevent diseases, thereby improving health and reduce ...
Lance Garrett Shaver, Amit Sundly, Abdullah Omar Saif   +2 more
doaj   +1 more source

Chemically modified guide RNAs to expand CRISPR functionality [PDF]

, 2022
CRISPR-Cas, a bacterial adaptive immune system against viruses, has been repurposed into a biological research tool that is invaluable due to its simplicity and programmability.
Taemaitree, Lapatrada
core   +1 more source