Results 21 to 30 of about 363,164 (302)
Nature Biomedical Engineering, 2020 In less than a decade, the genome-editing technology now recognized by the Nobel Prize in Chemistry has impacted the biological and biomedical sciences widely. What’s next for CRISPR in biomedicine?
openaire +2 more sources
Engineering Microbiology, 2023 Type III CRISPR-Cas10 systems employ multiple immune activities to defend their hosts against invasion from mobile genetic elements (MGEs), including DNase and cyclic oligoadenylates (cOA) synthesis both of which are hosted by the type-specific protein ...
Zhenxiao Yu +3 more
doaj +1 more source
Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing [PDF]
Arab Universities Journal of Agricultural Sciences, 2023 Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures.
Sally Hassan +5 more
doaj +1 more source
Current Protocols in Stem Cell Biology, 2016 AbstractCRISPR/Cas9‐gene editing has emerged as a revolutionary technology to easily modify specific genomic loci by designing complementary sgRNA sequences and introducing these into cells along with Cas9. Self‐cloning CRISPR/Cas9 (scCRISPR) uses a self‐cleaving palindromic sgRNA plasmid (sgPal) that recombines with short PCR‐amplified site‐specific ...
Arbab, M., Sherwood, R. I.
openaire +2 more sources
CRISPR-Cas Systems for Detection of Nucleic Acids: PCR Empire now has a Vigorous Rival! [PDF]
Translational Research in Urology, 2021 Rapid and accurate detection of nucleic acids is essential in many fields of modern biology and medical diagnosis of pathogens. PCR has some disadvantages for performing this task, and therefore demand for an alternative is highly appreciated.
Roham Deyhimfar
doaj +1 more source
Hot and crispy : CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricus [PDF]
, 2013 The CRISPR (clustered regularly interspaced short palindromic repeats) and Cas (CRISPR-associated) genes are widely spread in bacteria and archaea, representing an intracellular defence system against invading viruses and plasmids.
White, Malcolm F., Zhang, Jing
core +2 more sources
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
RNA Biology, 2013 CRISPR loci consist of an array of short repeats separated by spacer sequences that match the genome of viruses and plasmids that infect prokaryotes. Transcription of the CRISPR array generates small antisense RNAs that mediate immunity against these invaders.
Inbal, Maniv +2 more
openaire +2 more sources
CRISPR-based diagnostics [PDF]
Nature Biomedical Engineering, 2021 The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel.
Michael M. Kaminski +4 more
openaire +4 more sources
Book Review: “The New Microbiology: From Microbiomes to CRISPR”
Annals of Clinical Microbiology, 2022 At the time when owing to COVID-19 pandemic, the public is more interested in infectious diseases and pathogens than in any other period, a book titled “The New Microbiology: From Microbiomes to CRISPR” by the renowned microbiologist Pascale Cossart was ...
Jung Oak Kang
doaj +1 more source