Results 81 to 90 of about 277,742 (368)

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Science, 2016
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive immune systems, which protect microbes against viruses and other foreign DNA.
Omar O. Abudayyeh, J. Gootenberg, S. Konermann, J. Joung, I. Slaymaker, David B. T. Cox, Sergey A. Shmakov, K. Makarova, E. Semenova, L. Minakhin, K. Severinov, A. Regev, E. Lander, E. Koonin, Feng Zhang   +14 more
semanticscholar   +1 more source

RNA Interference in the Age of CRISPR: Will CRISPR Interfere with RNAi? [PDF]

International Journal of Molecular Sciences, 2016
The recent emergence of multiple technologies for modifying gene structure has revolutionized mammalian biomedical research and enhanced the promises of gene therapy. Over the past decade, RNA interference (RNAi) based technologies widely dominated various research applications involving experimental modulation of gene expression at the post ...
Unniyampurath, U, Pilankatta, R, Krishnan, M.N   +2 more
openaire   +3 more sources

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

Molecular Oncology, EarlyView.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser, Florian Krumbach, Eyleen Corrales, Geoffroy Andrieux, Christian Preisinger, Franziska Liss, Alexandra Golzmann, Melanie Boerries, Kerstin Becker, Ruth Knüchel, Stefan Garczyk, Bernhard Lüscher   +11 more
wiley   +1 more source

Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells

Molecular Oncology, EarlyView.
Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, Gökhan Karakülah, Serif Senturk   +11 more
wiley   +1 more source

Nonclassical phase diagram for virus bacterial co-evolution mediated by CRISPR [PDF]

J. Roy. Soc. Interface 14 (2017) 20160905, 2017
CRISPR is a newly discovered prokaryotic immune system. Bacteria and archaea with this system incorporate genetic material from invading viruses into their genomes, providing protection against future infection by similar viruses. The conditions for coexistence of prokaryots and viruses is an interesting problem in evolutionary biology.
arxiv   +1 more source

High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

Nature Biotechnology, 2013
Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell–based reporter assay to characterize off-target cleavage
Yanfang Fu, Jennifer A. Foden, Cyd Khayter, Morgan L. Maeder, D. Reyon, J., Keith Joung, Jeffry D. Sander   +7 more
semanticscholar   +1 more source

CRISPR/Cas-based customization of pooled CRISPR libraries

PLOS ONE, 2018
Pooled CRISPR libraries are widely used in high-throughput screening to study various biological processes. Various pooled CRISPR libraries have been shared for CRISPR screens and useful tools have been developed to construct researcher's own libraries, however, many researchers are struggling to create their own pooled CRISPR libraries: it is a time ...
Jiyeon Kweon, Da-eun Kim, An-Hee Jang, Yongsub Kim   +3 more
openaire   +5 more sources

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, EarlyView.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

CRISPR-based assay reveals SARS-CoV-2 RNA dynamic changes and redistribution patterns in non-human primate model

Emerging Microbes and Infections, 2022
Mounting evidence indicates that SARS-CoV-2 can infect multiple systemic tissues, but few studies have evaluated SARS-CoV-2 RNA dynamics in multiple specimen types due to their reduced accessibility and diminished performance of RT-qPCR with non ...
Zhen Huang, Lili Zhang, Christopher J. Lyon, Bo Ning, Brady M. Youngquist, Alex Niu, Brandon J. Beddingfield, Nicholas J. Maness, Nakhle S. Saba, Chen-Zhong Li, Chad J. Roy, Tony Y. Hu   +11 more
doaj   +1 more source

A small PAM optimises target recognition in the CRISPR-Cas immune system [PDF]

arXiv, 2021
CRISPR-Cas is an adaptive immune mechanism that has been harnessed for a variety of genetic engineering applications: the Cas9 protein recognises a 2-5nt DNA motif, known as the PAM, and a programmable crRNA binds a target DNA sequence that is then cleaved.
arxiv