Results 81 to 90 of about 358,387 (202)

Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9. [PDF]

, 2020
CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure.
Hsu, Rohaine V, Medrano, Marco A, Mitchell, Brandon P, Narkhede, Yogesh B, Palermo, Giulia, Zewde, Nehemiah T   +5 more
core  

Rapid one-tube RPA-coupled CRISPR/Cas12a-based RID-MyC assay for the diagnosis of fungal keratitis

Indian Journal of Ophthalmology
Purpose: This study introduces and evaluates the single-tube rapid identification of mycoses using CRISPR (ST-RID-MyC) assay. This novel diagnostic tool combines recombinase polymerase amplification (RPA) with CRISPR/Cas12a for the rapid and precise ...
Hanith R Deivarajan, Kanmani Senthilkumar, Hari V Sekar, Vignesh Elamurugan, Jaishree Pandian, Anitha Venugopal, Dharmalingam Kuppamuthu, Lalitha Prajna, Venkatesh N Prajna, Siddharth Narendran   +9 more
doaj   +1 more source

Systems Analysis Implicates WAVE2 Complex in the Pathogenesis of Developmental Left-Sided Obstructive Heart Defects. [PDF]

, 2020
Genetic variants are the primary driver of congenital heart disease (CHD) pathogenesis. However, our ability to identify causative variants is limited.
Bernstein, Daniel, Bisgrove, Brent W, Brueckner, Martina, Chung, Wendy K, Deanfield, John, Demarest, Bradley, Edwards, Jonathan J, Fernandez, Nicolas F, Gelb, Bruce D, Giardini, Alessandro, Goldmuntz, Elizabeth, Kim, Richard, Lachmann, Alexander, Lifton, Richard P, Ma'ayan, Avi, Newburger, Jane W, Porter, George, Roberts, Amy E, Rouillard, Andrew D, Seidman, Christine E, Shankaran, Sunita S, Srivastava, Deepak, Tristani-Firouzi, Martin, Turan, Nahid, Wang, Zichen, Yost, H Joseph   +25 more
core  

Surprising CRISPR roadblocks [PDF]

Nature Methods, 2018
Cas9 induces larger-than-anticipated mutations in mouse and human cells. In the latter, efficient editing depends on inhibition of the DNA-damage-repair protein p53.
openaire   +2 more sources

CRISPR-controlled proteases

Biochemical Society Transactions
With the discovery of CRISPR-controlled proteases, CRISPR–Cas has moved beyond mere nucleic acid targeting into the territory of targeted protein cleavage. Here, we review the understanding of Craspase, the best-studied member of the growing CRISPR RNA-guided protease family.
Sam P. B. van Beljouw, Stan J. J. Brouns
openaire   +2 more sources

274 Development of CTX112 a next generation allogeneic multiplexed CRISPR-edited CART cell therapy with disruptions of the TGFBR2 and Regnase-1 genes for improved manufacturing and potency

Journal for ImmunoTherapy of Cancer, 2023
Sarah Cohen, Jacob Waldman, Andrew Dunn, Thao Nguyen, Jonathan Terrett, Brigid Mcewan, Meghna Kuppuraju, Mohammed Ghonime, Robert Chain, Zinkal Padalia, Demetrios Kalaitzidis, Elizabeth Koch, Lauren Zakas, Nivedita Jaishankar, Davis Settipane, Paul Tetteh, Mary-Lee Dequeant, Sushant Karnik, Chandirasegaran Massilamany, Henia Dar, Melanie Allen, Laura Serwer, Melanie Butler-Gauthier, Parin Sripakdeevong, Shashwat Nagar, Yin Tang, Nicole Flanagan, Elaine Huang, Shashwant Phuyal, Erisa Sula, Maria Lei Zhang, Cristian Loaiza, Erin Thorstensen, Keith Steiger, Katie Schum, Kayla Urbaez, Anna Ma, Annie Yang Weaver, Christopher Finch, PK Morrow, Mark Benton   +40 more
doaj   +1 more source

Bacteria herald a new era of gene editing. [PDF]

, 2013
The demonstration that nucleases guided by bacterial RNA can disrupt human genes represents a landmark in the rapidly developing field of genome ...
Segal, David J
core  

324 Allogeneic CAR T cells targeting Liv-1 for breast cancer

Journal for ImmunoTherapy of Cancer, 2023
Minh Pham, Jonathan Terrett, Demetrios Kalaitzidis, Jason Sagert, Lauren Zakas, Laura Bartle, Zejun Li, Kelly Maeng, Luke Hanley, Avanti Ganpule   +9 more
doaj   +1 more source

Genome editing from Cas9 to IscB: Backwards and forwards towards new breakthroughs

Engineering Microbiology, 2021
Zhenxiao Yu, Qunxin She
doaj   +1 more source

Editing of the MALAT1 Gene in MDA-MB-361 Breast Cancer Cell Line using the Novel CRISPR Method

Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām, 2022
Introduction: Long non-coding RNAs play an important role in regulating gene expression, RNA processing, histone modification, and rearrangement of chromatin genes. These molecules can also be involved in many biological processes, such as organogenesis,
Soraya Ahmadi-Baloutaki, Abbas Doosti, mojtaba Jaafarinia, Hamedreza Goudarzi   +3 more
doaj