Results 121 to 130 of about 3,782 (173)

Auxotrophic Mutants of Crithidia fasciculata

The Journal of Parasitology, 1990
In pursuance of genetic studies, after exposure to ethylmethanesulfonate, 11 auxotrophic mutants of Crithidia fasciculata were cloned. Three proved uracil dependent; 3 serine dependent; and the remainder have not had their auxotrophy defined.
L A, Garcia, I, Roitman
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Glucose transport in Crithidia luciliae

Molecular and Biochemical Parasitology, 1992
The glucose analogue, 2-deoxy-D-glucose, was used to characterise the glucose transport system in Crithidia luciliae choanomastigotes. Uptake was temperature dependent with a Q10 of 2, and saturable with a Km of 0.22 mM and Vmax of 5.5 nmol min-1 (mg protein)-1 at 23 degrees C.
L A, Knodler, P J, Schofield, M R, Edwards   +2 more
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Crithidia fasciculata: Characterization of polysaccharide

Experimental Parasitology, 1972
Abstract A carbohydrate contaminant was discovered in phenol extracts of the insect hemoflagellate, Crithidia fasciculata . Ion-exchange chromatography was used to separate the polysaccharide from the cellular RNA. Chromatographic, electrophoretic, and colorimetric analyses of the hydrolyzed polysaccharide indicate that it is composed of mannose.
M, Gottlieb, P A, Lanzetta, J, Berech
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Serum Crithidia levels in disease

Biochemical Medicine, 1980
Abstract Previous papers recorded levels of crithidia active compounds in sera of normal and diseased subject (1,2). Elucidation of the primary metabolic defect in malignant hyperphenyl-alaniaemia as greatly reduced cell levels of tetrahydrobiopterin, arising from either inadequate biosynthesis or its diminished regeneration from quinonoid ...
R J, Leeming, J A, Blair
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Oxidative phosphorylation in Crithidia fasciculata

Biochemical and Biophysical Research Communications, 1967
Abstract Respiratory chain-linked phosphorylation has been demonstrated in cell-free extracts from the protozoan Crithidia fasciculata . The preparations were capable of coupling phosphate esterification to respiration, but were not “tightly coupled”.
J J, Toner, M M, Weber
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The Purine Phosphoribosyltransferases of Crithidia fasciculata

The Journal of Parasitology, 1979
The purine phosphoribosyltransferases of Crithidia fasciculata were identified and some of their properties described. The organism possesses three separate enzymes for the production of AMP, IMP, and GMP. The evidence for this comes from the observed differences in elution patterns from gel filtration columns, differences in heat sensitivity, and ...
G W, Kidder, L L, Nolan, V C, Dewey
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Biosynthesis of phosphatidylinositol in Crithidia fasciculata

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
Microsomal preparations from the protozoan (Crithidia fasciculata were shown to incorporate myo-[2-3H]inositol into phosphatidylinositol by both the CDPdiacylglycerol:myo-inositol phosphatidyltransferase reaction and by a myo-inositol exchange reaction. Non-ionic detergent and Mg2+ were necessary for the measurement of transferase activity.
C J, Daniels, F B, Palmer
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Glucosylation of glycoproteins in Crithidia fasciculata

Molecular and Biochemical Parasitology, 1991
High mannose-type, N-linked oligosaccharides devoid of glucose units may be glucosylated directly from UDP-Glc in mammalian, plant, fungal and protozoan cells. The glucosylated compounds thus formed (protein-linked Glc1Man5-9GlcNAc2, depending on the organisms) are immediately deglucosylated by glucosidase II, an enzyme located, the same as the ...
G, Gotz, S, Gañán, A J, Parodi
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Aerobic metabolism of Crithidia fasciculata

Experimental Parasitology, 1960
1. 1. Asparagine, aspartate, glucose, glutamate, glutamine, alanine, isocitrate, and oxalacetate (at pH 7.4) stimulated the oxygen consumption of intact cells of C. fasciculata. 2. 2. Krebs' tricarboxylic acid cycle intermediates (excepting isocitrate and oxalacetate) did not stimulate the oxygen consumption of the intact cells at the same pH.
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Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi

Monoxenous trypanosomatids exclusively parasitize insects and are powerful systems for exploring trypanosomatid biology, the evolution of parasitism, and host-parasite interactions. In addition, they are tractable early-diverging eukaryotes that can serve as model organisms for a variety of conserved processes.
Blyssalyn V, Bieber, Faith A, St Clair, Jesse, Quatse, Shane, Denecke, Michael, Povelones, Megan L, Povelones   +5 more
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