Results 131 to 140 of about 1,687 (191)

A new expression vector for Crithidia fasciculata and Leishmania

open access: yesMolecular and Biochemical Parasitology, 2002
Crithidia fasciculata is a monogenetic parasite of insects. It grows in fully defined media without requiring serum, which facilitates biochemical analysis. We have constructed a series of expression systems that allows expression of transfected genes in the kinetoplastid protozoa Crithidia and Leishmania.
Tetaud, Emmanuel   +4 more
openaire   +4 more sources

Ornithine decarboxylase fromCrithidia fasciculatais metabolically unstable and resistant to polyamine down-regulation

open access: yesFEBS Letters, 1992
Ornithine decarboxylase (ODC) of Crithidia fasciculata extracts shows maximal activity during exponential growth of the parasite and decreases markedly in the stationary phase.
Nélida S Gonzalez, Israel D Algranati
exaly   +3 more sources

Extremely rapid turnover of S-adenosylmethionine decarboxylase in Crithidia fasciculata

open access: yesFEBS Letters, 2003
The activity of S-adenosylmethionine decarboxylase (AdoMetDC) in Crithidia fasciculata was shown to be correlated to the growth of the parasite. An increase in activity was observed during exponential growth.
Lo Persson
exaly   +2 more sources

Auxotrophic Mutants of Crithidia fasciculata

The Journal of Parasitology, 1990
In pursuance of genetic studies, after exposure to ethylmethanesulfonate, 11 auxotrophic mutants of Crithidia fasciculata were cloned. Three proved uracil dependent; 3 serine dependent; and the remainder have not had their auxotrophy defined.
L A, Garcia, I, Roitman
openaire   +2 more sources

Crithidia fasciculata: Characterization of polysaccharide

Experimental Parasitology, 1972
Abstract A carbohydrate contaminant was discovered in phenol extracts of the insect hemoflagellate, Crithidia fasciculata . Ion-exchange chromatography was used to separate the polysaccharide from the cellular RNA. Chromatographic, electrophoretic, and colorimetric analyses of the hydrolyzed polysaccharide indicate that it is composed of mannose.
M, Gottlieb, P A, Lanzetta, J, Berech
openaire   +2 more sources

Oxidative phosphorylation in Crithidia fasciculata

Biochemical and Biophysical Research Communications, 1967
Abstract Respiratory chain-linked phosphorylation has been demonstrated in cell-free extracts from the protozoan Crithidia fasciculata . The preparations were capable of coupling phosphate esterification to respiration, but were not “tightly coupled”.
J J, Toner, M M, Weber
openaire   +2 more sources

The Purine Phosphoribosyltransferases of Crithidia fasciculata

The Journal of Parasitology, 1979
The purine phosphoribosyltransferases of Crithidia fasciculata were identified and some of their properties described. The organism possesses three separate enzymes for the production of AMP, IMP, and GMP. The evidence for this comes from the observed differences in elution patterns from gel filtration columns, differences in heat sensitivity, and ...
G W, Kidder, L L, Nolan, V C, Dewey
openaire   +2 more sources

Biosynthesis of phosphatidylinositol in Crithidia fasciculata

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
Microsomal preparations from the protozoan (Crithidia fasciculata were shown to incorporate myo-[2-3H]inositol into phosphatidylinositol by both the CDPdiacylglycerol:myo-inositol phosphatidyltransferase reaction and by a myo-inositol exchange reaction. Non-ionic detergent and Mg2+ were necessary for the measurement of transferase activity.
C J, Daniels, F B, Palmer
openaire   +2 more sources

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