Results 31 to 40 of about 14,077 (260)

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

open access: yesFEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Specimen Identification Through Multilocus Species Tree Constructed From Single‐Copy Orthologs (SCOs): A Case Study in Cymbidium Subgenus Jensoa

open access: yesEcology and Evolution
Standard barcodes and ultra‐barcode encounter significant challenges when delimiting and discriminating closely related species characterized by deep coalescence, hybrid speciation, gene flow, or low sequence variation.
Zheng‐Shan He   +4 more
doaj   +1 more source

An inventory of crop wild relatives and wild‐utilized plants in Canada

open access: yesCrop Science, 2022
AbstractIn the face of global pressures of change and biodiversity loss, crop wild relatives (CWR) and wild‐utilized species (WUS) urgently require conservation attention. To advance conservation, we assembled a national inventory of CWR and WUS in Canada.
Jens C. Ulrich   +7 more
openaire   +2 more sources

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

open access: yesFEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee   +7 more
wiley   +1 more source

Multiple Glacial Refugia and Complex Postglacial Dynamics of Primula sikkimensis (Primuaceae) in the Heterogeneous Qinghai‐Tibet Plateau

open access: yesEcology and Evolution
The Qinghai‐Tibet Plateau (QTP) is a global biodiversity hotspot where Quaternary climatic oscillations profoundly shaped the evolution of endemic alpine flora.
Hua‐Ying Sun   +10 more
doaj   +1 more source

Breaking the soil barrier: Somatic embryogenesis and polyploidization enable high-yield saponin in Panax notoginseng (Burk.) F.H.Chen

open access: yesIndustrial Crops and Products
Panax notoginseng (Burk.) F.H.Chen is a highly valuable medicinal plant. The saponins abundant in its roots and rhizomes are effective in medicine, health products, and skincare. However, its planting faces severe continuous-cropping obstacles, high soil-
Xue He   +8 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

open access: yesFEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

A high-quality chromosome-level genome assembly of herbaceous bamboo species Lithachne pauciflora

open access: yesScientific Data
Lithachne pauciflora is a herbaceous bamboo species characterized by its short height, low degree of lignification, and dimorphic florets, making it a special model for studying the key trait evolution in bamboo.
Ke-Cheng Qian   +4 more
doaj   +1 more source

Dispersed repeats and inverted repeat expansion drive major plastomic rearrangements in Calliandra haematocephala (Leguminosae: Mimoseae)

open access: yesFrontiers in Plant Science
Structural variations in legume plastomes impact phylogenetic and evolutionary studies. In this study, we focus on the tribe Mimoseae by integrating a newly assembled plastome of Calliandra haematocephala (from PacBio sequencing data) with 15 previously ...
Libo Zhao   +15 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

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