Insights into Transient Dimerisation of Carnitine/Acylcarnitine Carrier (SLC25A20) from Sarkosyl/PAGE, Cross-Linking Reagents, and Comparative Modelling Analysis [PDF]
BiomoleculesThe carnitine/acylcarnitine carrier (CAC) is a crucial protein for cellular energy metabolism, facilitating the exchange of acylcarnitines and free carnitine across the mitochondrial membrane, thereby enabling fatty acid β-oxidation and oxidative ...
Nicola Giangregorio +3 more
doaj +4 more sources
Synthesis of Bioconjugation Reagents for Use in Covalent Cross-Linking of Proteins by Azide-Alkyne Cycloaddition [PDF]
MoleculesA series of azide- and cyclooctyne-functionalized N-hydroxysuccinimidyl esters (NHS esters) and benzotriazolides were prepared and used as N-acylation reagents to obtain azide-(BSA-1) and cyclooctyne-functionalized bovine serum albumin proteins (BSA-2 ...
Nadja Suhorepec +6 more
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Cysteine-enabled cleavability to advance cross-linking mass spectrometry for global analysis of endogenous protein-protein interactions [PDF]
Nature CommunicationsCross-linking mass spectrometry (XL-MS) is a powerful technology for probing protein-protein interactions (PPIs) and elucidating architectures of protein complexes at the systems level. While successful, the proteome coverage remains limited.
Fenglong Jiao +6 more
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Bi-functional cross-linking reagents efficiently capture protein-DNA complexes in Drosophila embryos. [PDF]
Fly (Austin), 2014 Aoki T +5 more
europepmc +3 more sources
Trioxane-based MS-cleavable cross-linking mass spectrometry for profiling multimeric interactions of cellular networks [PDF]
Nature CommunicationsCross-linking mass spectrometry (XL-MS) is a powerful technology for mapping protein-protein interactions (PPIs) at the systems level. While bivalent cross-links are effective for defining protein interactions and structures, multivalent cross-links ...
Clinton Yu +8 more
doaj +2 more sources
Advancements and Perspectives in the Bioprosthetic Heart Valve: A Comprehensive Review on Biomaterial Processing and Emerging Polymeric Materials [PDF]
Journal of the American Heart Association: Cardiovascular and Cerebrovascular DiseaseHeart valve disease contributes to cardiovascular disease–associated death. Bioprosthetic heart valves have emerged as a preferred option for heart valve replacement due to their superior hemodynamic performance and reduced need for lifelong ...
Hanluo Li +10 more
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Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions [PDF]
, 2012 We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs).
Chang Ho Sohn +11 more
openalex +5 more sources
An integrative structural biology approach to identify the binding mode of a nanobody towards the pea ascorbate peroxidase [PDF]
Computational and Structural Biotechnology JournalThe optimization of diagnostic devices such as biosensors often requires understanding the molecular details of the interaction between capture and target biomolecules. This can be experimentally obtained by cryo-electron microscopy, the preferred method
Claudia D’Ercole +3 more
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Food Chemistry: X, 2023 Generally, gelatin was irreversibly cross-linked by chemical reagents to improve its water-resistance. However, few chemical reagents meet both the requirements of high cross-inking efficiency and tunable degradation.
Shengbin He +7 more
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Molecules, 2022 Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition.
Mayya V. Monakhova +9 more
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