Results 111 to 120 of about 170,253 (336)
In this study, a new type of bioactive glass fiber ‐based composite magnesium phosphate bone cement is prepared and verified that its mechanical strength and biological properties. In addition, the cement may have played a biologically active role in the Notch and HIF signaling pathways.
Yuzheng Lu+12 more
wiley +1 more source
This study develops a tissue‐engineered model of the atherosclerotic cap using human mesenchymal stromal cells (MSCs). After 2 weeks of culture to produce a collagenous matrix, a mineralizing medium induces microcalcifications over 4 weeks. These constructs, imaged with second harmonic generation microscopy, lead to reduced ultimate stress at rupture ...
Imke L. Jansen+4 more
wiley +1 more source
E. coli-quantum dot bioconjugates as whole-cell fluorescent reporters for probing cellular damage [PDF]
A quantum dot (QD) conjugated whole-cell E. coli biosensor (E. coli–QD bioconjugates) was developed as a new molecular tool for probing cellular damage. The E. coli–QD bioconjugates were viable and exhibited fluorescence emission at 585 nm.
Chouhan, Raghuraj Singh+2 more
core +1 more source
The glomerular filtration barrier (GFB) is the first step of blood filtration by the kidneys. The concerning increase of kidney diseases makes the development of new models essential. In this context, microphysiological glomerular filtration barriers focus on closely reproducing the physiological architecture of the in vivo GFB: podocytes, glomerular ...
Manon Miran+5 more
wiley +1 more source
Western Blotting and Immunoprecipitation of Native Human PIEZO1 Channels
PIEZO1 is a mechanically activated ion channel essential for mechanotransduction and downstream signaling in almost all organ systems. Western blotting is commonly used to study the expression, stability, and post-translational modifications of proteins.
Jinyuan Vero Li+2 more
doaj +1 more source
Investigation of the organization of rhodopsin in the sheep photoreceptor membrane by using cross-linking reagents [PDF]
M. Brett, J. B. C. Findlay
openalex +1 more source
A detailed workflow for recombinant GALC production and characterization is presented to support enzyme replacement therapy for Krabbe disease. In vitro assays demonstrate that physiological GALC doses restore enzymatic activity and autophagic flux without affecting cell viability, whereas higher doses impair autophagy and reduce viability.
Ambra Del Grosso+5 more
wiley +1 more source
The development of SS′-polymethylenebis(methanethiosulphonates) as reversible cross-linking reagents for thiol groups and their use to form stable catalytically active cross-linked dimers within glyceraldehyde 3-phosphate dehydrogenase [PDF]
David P. Bloxham, Ram Prakash Sharma
openalex +1 more source
Residual Stress States in Microstructurally Graded PBF–LB/M Austenitic Steel Components
This study examines microstructurally graded 316L rectangular tube profiles fabricated via PBF–LB/M using a dual‐laser system. A 1 kW top‐hat and a 400 W Gaussian laser create distinct grain sizes and crystallographic texture. Mechanical properties are linked to microstructural evolution driven by processing conditions.
Nico Möller+5 more
wiley +1 more source
In this study, the interplay of dipolar dynamics and ionic charge transport in MOF compounds is investigated. Synthesizing the novel structure CFA‐25 with integrated freely rotating dipolar groups, local and macroscopic effects, including interactions with Cs cations are explored.
Ralph Freund+6 more
wiley +1 more source