Results 11 to 20 of about 490,027 (386)

Insights into Transient Dimerisation of Carnitine/Acylcarnitine Carrier (SLC25A20) from Sarkosyl/PAGE, Cross-Linking Reagents, and Comparative Modelling Analysis [PDF]

open access: goldBiomolecules
The carnitine/acylcarnitine carrier (CAC) is a crucial protein for cellular energy metabolism, facilitating the exchange of acylcarnitines and free carnitine across the mitochondrial membrane, thereby enabling fatty acid β-oxidation and oxidative ...
Nicola Giangregorio   +3 more
doaj   +3 more sources

A Cofactor Regeneration System for 2‐Aminobutyric Acid Production Based on Combined Cross‐Linked Enzyme Aggregates: Utilizing His‐Tagged Enzymes With Low‐Concentration Calcium Ions as Precipitant [PDF]

open access: yesEngineering in Life Sciences
Combined cross‐linked enzyme aggregates (combi‐CLEAs) represent a promising carrier‐free immobilized enzyme technology. This study describes the preparation of combi‐CLEAs comprising leucine dehydrogenase (LeuDH) and formate dehydrogenase (FDH) for the ...
Jingran Liu   +8 more
doaj   +2 more sources

Synthesis and Conformational Analysis of Novel Trimeric Maleimide Cross-Linking Reagents

open access: yesThe Journal of Organic Chemistry, 2007
Nine homotrifunctional cross-linking reagents are presented. Their synthesis and chemical properties as well as their characterization by classical mechanical conformational searching techniques is reported. Mixed Low Mode and Monte Carlo searching techniques were used to exhaustively sample the OPLS2005/GBSA(water) potential energy surface of ...
José L. Espartero   +6 more
openaire   +4 more sources

Reactive Acrylamide-Modified DNA Traps for Accurate Cross-Linking with Cysteine Residues in DNA–Protein Complexes Using Mismatch Repair Protein MutS as a Model

open access: yesMolecules, 2022
Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition.
Mayya V. Monakhova   +9 more
doaj   +1 more source

Mimicked synthetic ribosomal protein complex for benchmarking crosslinking mass spectrometry workflows

open access: yesNature Communications, 2022
Cross-linking mass spectrometry is widely used to elucidate protein structures and interactions. Here, the authors generate an extensive peptide library to benchmark the most common cross-link search engines with frequently used cross-linking reagents in
Manuel Matzinger   +5 more
doaj   +1 more source

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

open access: yesNanomaterials, 2023
The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability
Alexandra Virginia Bounegru   +1 more
doaj   +1 more source

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