Nature Communications, 2022 Cross-linking mass spectrometry is widely used to elucidate protein structures and interactions. Here, the authors generate an extensive peptide library to benchmark the most common cross-link search engines with frequently used cross-linking reagents in
Manuel Matzinger +5 more
doaj +1 more source
Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review
Nanomaterials, 2023 The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability
Alexandra Virginia Bounegru +1 more
doaj +1 more source
Ligand-induced movements of inner transmembrane helices of Glut1 revealed by chemical cross-linking of di-cysteine mutants. [PDF]
PLoS ONE, 2012 The relative orientation and proximity of the pseudo-symmetrical inner transmembrane helical pairs 5/8 and 2/11 of Glut1 were analyzed by chemical cross-linking of di-cysteine mutants.
Mike Mueckler, Carol Makepeace
doaj +1 more source
Application of proanthocyanidin in the treatment and repair of deep caries
口腔疾病防治, 2023 Proanthocyanidin (PA), as a kind of natural plant polyphenol, have a variety of biological functions, such as promoting remineralization, inducing collagen cross-linking, inhibiting protease activity and inhibiting bacteria.
GAO Ying +7 more
doaj +1 more source
ECL 3.0: a sensitive peptide identification tool for cross-linking mass spectrometry data analysis
BMC Bioinformatics, 2023 Background Cross-linking mass spectrometry (XL-MS) is a powerful technique for detecting protein–protein interactions (PPIs) and modeling protein structures in a high-throughput manner.
Chen Zhou +6 more
doaj +1 more source
The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents [PDF]
, 1990 Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V. Kurzchalia, E. Hartmarm, and T. A. Rapoport. 1987. Nature
Dobberstein, Bernhard +9 more
core +1 more source
Transmembrane helical interactions in the CFTR channel pore.
PLoS Computational Biology, 2017 Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene affect CFTR protein biogenesis or its function as a chloride channel, resulting in dysregulation of epithelial fluid transport in the lung, pancreas and other organs in ...
Jhuma Das +5 more
doaj +1 more source
INVESTIGATIONS ON THE EFFECT OF SODIUM TRIMETAPHOSPHATE AND SODIUM SULFATE CONCENTRATIONS ON THE PROPERTIES OF CROSS-LINKED CASSAVA STARCH FILMS [PDF]
Química NovaFilms based on pure native starch have limited applications, are easily breakable, very hygroscopic, and permeable to water vapor. One method for improving these properties is to modify starch by cross-linking.
Marisa Ferreira Karow +8 more
doaj +1 more source
, 2012 Chemical cross-linking in combination with mass spectrometric analysis offers the potential to obtain low-resolution structural information from proteins and protein complexes. Identification of peptides connected by a cross-link provides direct evidence
Aebersold, R. +6 more
core +3 more sources
Cross-linking of dermal sheep collagen using a water-soluble carbodiimide [PDF]
, 1996 A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC).
Dijkstra, P.J. +5 more
core +4 more sources