Insights into Transient Dimerisation of Carnitine/Acylcarnitine Carrier (SLC25A20) from Sarkosyl/PAGE, Cross-Linking Reagents, and Comparative Modelling Analysis [PDF]
The carnitine/acylcarnitine carrier (CAC) is a crucial protein for cellular energy metabolism, facilitating the exchange of acylcarnitines and free carnitine across the mitochondrial membrane, thereby enabling fatty acid β-oxidation and oxidative ...
Nicola Giangregorio+3 more
doaj +3 more sources
Bi-functional cross-linking reagents efficiently capture protein-DNA complexes in Drosophila embryos [PDF]
T. Aoki+5 more
semanticscholar +3 more sources
Bifunctional protein cross-linking reagents improve labeling of cytoskeletal proteins for qualitative and quantitative fluorescence microscopy. [PDF]
B. Safiejko-Mroczka, P. Bell
semanticscholar +2 more sources
A Cofactor Regeneration System for 2‐Aminobutyric Acid Production Based on Combined Cross‐Linked Enzyme Aggregates: Utilizing His‐Tagged Enzymes With Low‐Concentration Calcium Ions as Precipitant [PDF]
Combined cross‐linked enzyme aggregates (combi‐CLEAs) represent a promising carrier‐free immobilized enzyme technology. This study describes the preparation of combi‐CLEAs comprising leucine dehydrogenase (LeuDH) and formate dehydrogenase (FDH) for the ...
Jingran Liu+8 more
doaj +2 more sources
Localization of Membrane-Associated Proteins in Vesicular Stomatitis Virus by Use of Hydrophobic Membrane Probes and Cross-Linking Reagents [PDF]
J. Zakowski, R. Wagner
semanticscholar +2 more sources
H. Nygren, H. Hansson
semanticscholar +2 more sources
Synthesis and Conformational Analysis of Novel Trimeric Maleimide Cross-Linking Reagents
Nine homotrifunctional cross-linking reagents are presented. Their synthesis and chemical properties as well as their characterization by classical mechanical conformational searching techniques is reported. Mixed Low Mode and Monte Carlo searching techniques were used to exhaustively sample the OPLS2005/GBSA(water) potential energy surface of ...
José L. Espartero+6 more
openaire +4 more sources
Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition.
Mayya V. Monakhova+9 more
doaj +1 more source
Cross-linking mass spectrometry is widely used to elucidate protein structures and interactions. Here, the authors generate an extensive peptide library to benchmark the most common cross-link search engines with frequently used cross-linking reagents in
Manuel Matzinger+5 more
doaj +1 more source
Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review
The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability
Alexandra Virginia Bounegru+1 more
doaj +1 more source