Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review
Nanomaterials, 2023 The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability
Alexandra Virginia Bounegru +1 more
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Application of proanthocyanidin in the treatment and repair of deep caries
口腔疾病防治, 2023 Proanthocyanidin (PA), as a kind of natural plant polyphenol, have a variety of biological functions, such as promoting remineralization, inducing collagen cross-linking, inhibiting protease activity and inhibiting bacteria.
GAO Ying +7 more
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PhoX – an IMAC-enrichable Crosslinking Reagent [PDF]
ACS Central Science, 2019 AbstractChemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry.
Steigenberger, Barbara A. +3 more
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ECL 3.0: a sensitive peptide identification tool for cross-linking mass spectrometry data analysis
BMC Bioinformatics, 2023 Background Cross-linking mass spectrometry (XL-MS) is a powerful technique for detecting protein–protein interactions (PPIs) and modeling protein structures in a high-throughput manner.
Chen Zhou +6 more
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The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents [PDF]
, 1990 Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V. Kurzchalia, E. Hartmarm, and T. A. Rapoport. 1987. Nature
Dobberstein, Bernhard +9 more
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Cleavage and cross-linking of proteins with osmium (VII) reagents. [PDF]
Journal of Histochemistry & Cytochemistry, 1982 The specific cleavage of proteins following reaction with osmium tetroxide is slowed down by tertiary amines which are known to be good ligands for osmium. Bidentate ligands are more effective than monodentate ligands. These same ligands also promote an intermolecular cross-linking reaction.
E. J. Behrman, Michael Emerman
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Transmembrane helical interactions in the CFTR channel pore.
PLoS Computational Biology, 2017 Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene affect CFTR protein biogenesis or its function as a chloride channel, resulting in dysregulation of epithelial fluid transport in the lung, pancreas and other organs in ...
Jhuma Das +5 more
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Antigenic Properties of Fixed and Unfixed Particles of Some Cucumber Mosaic Virus Strains [PDF]
E3S Web of Conferences, 2020 The virus particle of Cucumber mosaic virus (CMV) is unstable and degrades during and after virus preparation. For longterm storage, either 0.25% glutaraldehyde or 0.2% formaldehyde are used as protein cross-linking reagents for stabilising the antigenic
Sri Wahyuni Wiwiek
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Development of a Novel Sulfoxide-Containing MS-Cleavable Homobifunctional Cysteine-Reactive Cross-Linker for Studying Protein–Protein Interactions [PDF]
, 2018 Cross-linking mass spectrometry (XL-MS) has become an emerging technology for defining protein-protein interactions (PPIs) and elucidating architectures of large protein complexes.
Block, Sarah A +6 more
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Mass Spectrometry Evidence for Cisplatin As a Protein Cross-Linking Reagent [PDF]
Analytical Chemistry, 2011 Cisplatin is a potent anticancer drug, which functions by cross-linking adjacent DNA guanine residues. However within 1 day of injection, 65-98% of the platinum in the blood plasma is protein-bound. It is generally accepted that cisplatin binds to methionine and histidine residues, but what is often underappreciated is that platinum from cisplatin has ...
Huilin Li +6 more
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