Results 251 to 260 of about 4,667,155 (305)

A new nucleic acid-protein cross-linking reagent

Molecular and General Genetics MGG, 1977
A new photoactivable reagent is described, which allows the formation of RNA-protein cross-links via disulfide bridges in combination with mercaptobutyrimidate. The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking.
Oste, Christian, Parfait, Raymond, Bollen, Alex, Crichton, Robert R.R.   +3 more
openaire   +3 more sources

An Allylstannane Reagent on Non-Cross-Linked Polystyrene Support

Organic Letters, 1999
[reaction: see text] A new allylstannane reagent on non-cross-linked polystyrene was developed for the first time. This support differs markedly from standard cross-linked polymers because it is completely soluble in organic solvents; moreover, the reactions can be conveniently monitored directly by standard 1H NMR methods.
E J, Enholm, M E, Gallagher, K M, Moran, J S, Lombardi, J P, Schulte   +4 more
openaire   +2 more sources

Novel DNA Cross-Linking Reagents

2016
Abstract DNA interstrand cross-links (ICLs) are the sources of the cytotoxicity of many anticancer agents. DNA cross-linking agents are used as anticancer agents, for DNA damage and repair study, for nucleic acid detection, and for construction of DNA nanomaterials.
Heli Fan, Xiaohua Peng
openaire   +1 more source

Accessibility and cross-linking of native neurofilaments to chemical reagents

International Journal of Biochemistry, 1984
Native neurofilaments were submitted to cross-linking reactions with bifunctional reagents (DMA, DMS and DSS) and to chemical reactions with sterically bulky reagents such as EEDQ and DTAF , as well as a glutaraldehyde-activated gel. The 160K and 70K neurofilament proteins reacted slightly more than the 210K neurofilament protein with DMS and DSS.
C, Francois, A, Delacourte, K K, Han, M, Mazzuca   +3 more
openaire   +2 more sources

An amino acid-based heterofunctional cross-linking reagent

Amino Acids, 2014
We describe the synthesis and characterization of a new lysine-based heterofunctional cross-linking reagent. It carries two readily available aminooxy functionalities and an activated and protected thiol group that is capable of generating reducible disulfides, the former enable bioorthogonal modification of ketones and aldehydes by the formation of an
Lelle, M., Peneva, K.
openaire   +3 more sources

Cross-linking reagents and membrane organization

Trends in Biochemical Sciences, 1979
Abstract Bifunctional cross-linking reagents have been widely used to study quaternary structure in oligomeric enzymes — their application to membrane systems involves a new level of problems but is proving informative.
openaire   +1 more source

Stabilisation of invertases by treatment with cross‐linking reagents

Journal of Chemical Technology and Biotechnology, 1979
AbstractCommercial baker's yeast invertase concentrate, used in the manufacture of a variety of confectionary products, was treated with various chemical cross‐linking agents in an attempt to improve its thermal stability to that of the more useful invertase from Candida utilis, which has been shown previously to have high thermal stability.
Jonathan Woodward, Alan Wiseman
openaire   +1 more source

Synthesis of a Water-Soluble Protein Cross-Linking Reagent

Biological Chemistry Hoppe-Seyler, 1990
2,4-Difluoro-5-nitrobenzenesulfonic acid has been synthesized by the sulfonation of 2,4-difluoronitrobenzene, and precipitated with KCl as the potassium sulfonate. The structure was confirmed by chemical and spectroscopic methods (IR, 1H-NMR, 13C-NMR, 19F-NMR, UV, MS and ultimate organic analysis). Lysozyme was cross-linked with the potassium sulfonate
openaire   +2 more sources

A new cleavable reagent for cross-linking and reversible immobilization of proteins

Biochemical and Biophysical Research Communications, 1979
Abstract We have prepared a new bifunctional reagent for the cross-linking and reversible immobilization of proteins through their amine groups. This compound, ethylene glycolyl bis(succinimidyl succinate), reacts rapidly with proteins, at pH 7 and at high dilution.
P M, Abdella, P K, Smith, G P, Royer
openaire   +2 more sources

The monothioacetal group, a new cleavable center for cross-linking reagents

Biochemical and Biophysical Research Communications, 1979
Abstract A protein cross-linking reagent which contains a monothioacetal moiety is described. Cross-links generated using this reagent may be specifically cleaved by dilute mercuric ion at neutral pH.
D C, Muchmore, F W, Dahlquist
openaire   +2 more sources