Results 271 to 280 of about 183,904 (311)
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A disulfide-bridge bifunctional imidoester as a reversible cross-linking reagent
Biochemical and Biophysical Research Communications, 1975Abstract A disulfide-bridged bifunctional imidoester, dimethyl 3, 3′ dithio-bispropionimidate (DTP) has been prepared and investigated as a reagent to introduce covalent cross-links in proteins that can subsequently be broken by mild reduction. Such reversible cross-links were shown to be introduced by DTP in the soluble subunit proteins aldolase and
A, Ruoho +3 more
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The oxidative cleavability of protein cross-linking reagents containing organoselenium bridges
Bioconjugate Chemistry, 1990The intensive use of cleavable cross-linking reagents to study macromolecular biological interactions has shown a demand for optimizing these reagents in such a way that the involved macromolecules remain intact. The present work focuses on the development of selenium linkers that are cleavable by mild oxidation.
T, Koch +3 more
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Synthesis and Conformational Analysis of Novel Trimeric Maleimide Cross-Linking Reagents
The Journal of Organic Chemistry, 2007Nine homotrifunctional cross-linking reagents are presented. Their synthesis and chemical properties as well as their characterization by classical mechanical conformational searching techniques is reported. Mixed Low Mode and Monte Carlo searching techniques were used to exhaustively sample the OPLS2005/GBSA(water) potential energy surface of ...
Agnieszka, Szczepanska +6 more
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The monothioacetal group, a new cleavable center for cross-linking reagents
Biochemical and Biophysical Research Communications, 1979Abstract A protein cross-linking reagent which contains a monothioacetal moiety is described. Cross-links generated using this reagent may be specifically cleaved by dilute mercuric ion at neutral pH.
D C, Muchmore, F W, Dahlquist
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A novel biotinylated heterobifunctional cross-linking reagent bearing an aromatic diazirine
Bioorganic & Medicinal Chemistry, 1994The synthesis of a p-[(3-trifluoromethyl)diazirine-3-yl]benzoic acid derivative is described as a new carbene generating heterobifunctional cross-linking reagent. The cross-linker carries a biotin moiety in order to make use of avidin-biotin technology for specific manipulation of cross-linked components.
Y, Hatanaka, M, Hashimoto, Y, Kanaoka
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A Novel Dual Cross-linking Reagent for Dentin Bonding Interface Stability
Journal of Dental ResearchThe cross-linking reagent has been proposed as a means of modifying dentin collagen, inhibiting matrix metalloproteinase activities, and enhancing bond durability during dentin bonding procedures. This study aimed to synthesize an operation-friendly dual cross-linking reagent—3-(4-formyphenoxy)-2-hydroxypropyl methacrylate (FPA)—to assess its ability ...
Z.L. Tian +6 more
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MODEL STUDIES OF POTENTIAL INTERMOLECULAR HEMOGLOBIN CROSS-LINKING REAGENTS
2012The search for a safe and effective alternative to whole blood or packed red blood cells (RBC) has long been of interest in the medical and scientific communities. Hemoglobin (Hb) is the oxygen-carrying protein found within RBCs, and blood substitutes based on stroma-free hemoglobin (SFHb) have been modified by cross-linking reagents so that the ...
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Synthesis and applications of heterobifunctional photocleavable cross-linking reagents.
Methods in enzymology, 1998This study designed, synthesized, and characterized a number of new heterobifunctional photocleavable cross-linking reagents that may be used to photomodulate the activity of proteins or to prepare caged fluorescent dyes. Biomolecules or fluorophores caged via a thiol group with the BNBASE reagent can be covalently linked to a second protein, ligand ...
G, Marriott, J, Ottl
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