Results 281 to 290 of about 397,014 (342)

Bifunctional imidoesters as cross-linking reagents

Biochemical and Biophysical Research Communications, 1966
Abstract Bifunctional small molecule reagents have been important in the study of a number of problems in protein chemistry (cf. Wold, 1961 a, b; Zahn and Meienhofer, 1958 ; Singer, 1959 ). For most purposes it is desirable that the chemical modifications produced by the bifunctional reagent cause as little alteration as possible in the conformation
Anne H. Dutton, Margie Adams, S. J. Singer   +2 more
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Chemical Cross-Linking: Reagents and Problems in Studies of Membrane Structure

Annual Review of Biochemistry, 1977
INTRODUCTION AND PERSPECTIVE ....... 524 THE PROCEDURE AND THE PROBLEMS. . . . . .... ...... . . .. . ... . . .... .... . 525 CHEMISTRY OF SELECTED REAGENTS 527 Alkyl Imidates (lmidoesters} 527 Synthesis 528 Reaction paths and mechanisms 528 Use as modifYing reagents 531 Acyl Azides 532 Sulfhydryl Reagents 532 Nitrenes 534 ...
Kevin Peters, Frederic M. Richards
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Cross-linking reagents and membrane organization

Trends in Biochemical Sciences, 1979
Abstract Bifunctional cross-linking reagents have been widely used to study quaternary structure in oligomeric enzymes — their application to membrane systems involves a new level of problems but is proving informative.
R. Freedman
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Physicochemical and structural characteristics of cross‐linked banana starch using three cross‐linking reagents

Starch - Stärke, 2010
AbstractBanana starch was cross‐linked using different cross‐linking reagents, phosphoryl chloride (POCl3), sodium trimetaphosphate (STMP), and epichlorohydrin (ECH), under alkaline conditions. The reaction conditions were selected to produce similar pasting profiles.
Luis A. Bello-Pérez, Ya-Jane Wang, Carmen Núñez-Santiago, M.L. Rodríguez-Marín   +3 more
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Synthesis and Photochemistry of a New Class of Photocleavable Protein Cross‐linking Reagents

Chemistry – A European Journal, 2004
AbstractA new series of photocleavable protein cross‐linking reagents based on bis(maleimide) derivatives of diaryl disulfides have been synthesised. They have been functionalised with cysteine and transient absorption spectra for the photolysis reaction have been recorded by using the pump‐probe technique with a time resolution of 100 femtoseconds ...
Milanesi, Lilia, Reid, Gavin D., Beddard, Godfrey S., Hunter, Christopher A., Waltho, Jonathan P.   +4 more
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Stabilization of restriction endonuclease Bam HI by cross‐linking reagents

Biotechnology and Bioengineering, 1989
AbstractBacillus amyloliquefaciens H produces a restriction endonuclease enzyme BamHl which is heat labile even at low temperatures. Studies were conducted to enhance thermal stability of BamHl using cross‐linking reagents, namely, glutaraldehyde, dimethyl adipimidate (DMA), dimethyl suberimidate (DMS), and dimethyl 3,3′‐dithiobispropionimidate (DTBP).
Virendra S. Bisaria, S. N. Mukhopadhyay, Ashok K. Dubey, T. K. Ghose   +3 more
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Effect of protein cross-linking reagents on membrane currents of squid axon

American Journal of Physiology-Cell Physiology, 1980
The effects of the protein cross-linking reagents glutaraldehyde, tannic acid, and formaldehyde were examined in voltage-clamped and internally perfused squid axons. All three reagents reduced sodium and potassium currents and reduced or abolished sodium inactivation. Glutaraldehyde and tannic acid produced an alteration of potassium current kinetics.
R. Horn, D. C. Eaton, M. S. Brodwick
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Studies with Cross-Linking Reagents on the Oligomeric Form of the Paramyxovirus Fusion Protein

Virology, 1994
The oligomeric form of the paramyxovirus simian virus 5 (SV5) fusion (F) glycoprotein has been examined by using chemical cross-linking and sucrose density gradient fractionation. In addition, chemical cross-linking was used to examine the kinetics of assembly of the F oligomer.
Reay G. Paterson, Robert A. Lamb, Rosemary Russell   +2 more
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The oxidative cleavability of protein cross-linking reagents containing organoselenium bridges

Bioconjugate Chemistry, 1990
The intensive use of cleavable cross-linking reagents to study macromolecular biological interactions has shown a demand for optimizing these reagents in such a way that the involved macromolecules remain intact. The present work focuses on the development of selenium linkers that are cleavable by mild oxidation.
Ulla Henriksen, Elisabeth Suenson, Troels Koch, Ole Buchardt   +3 more
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Membrane permeability of bifunctional, amino site‐specific, cross‐linking reagents

Journal of Supramolecular Structure, 1978
AbstractThe membrane permeability of a series of reversible cross‐linking reagents which are diazide tartarate derivatives has been compared with that of dimethyl‐3,3′‐dithiobispropionimidate (DTBP). The diazide tartarate derivatives tested include tartryl‐diazide (TDA), tartryl‐di(glycylazide) (TDGA), tartryl‐di(β‐alanylazide) (TDAA), tartryl‐di‐(γ ...
Larry J. Takemoto, Tokichi Miyakawa, C. Fred Fox   +2 more
openaire   +4 more sources