Results 251 to 260 of about 542,780 (295)

Th1/Th2 response profiles to the major allergens Cry j 1 and Cry j 2 of Japanese cedar pollen

Allergy, 1996
Cry j 1 and Cry j 2 are known to be the major allergens of Japanese cedar pollen. A comparative study was carried out on the immune responses to stimulation with Cry j 1 and Cry j 2 in 24 symptomatic patients and six nonallergic subjects. In T‐cell proliferation assays, mean stimulation indexes (SI) were 10.6 for Cry j 1 and 11.7 for Cry j 2 ...
K, Sugimura, S, Hashiguchi, Y, Takahashi, K, Hino, Y, Taniguchi, M, Kurimoto, K, Fukuda, M, Ohyama, G, Yamada   +8 more
openaire   +2 more sources

Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide

Comparative Immunology, Microbiology and Infectious Diseases, 2011
We determined whether a major Japanese cedar pollen allergen (Cry j 1) conjugated with CpG oligodeoxynucleotide would enhance allergen-specific Th1 responses in mice. Cry j 1 conjugated with CpG (Cry j 1-CpG) induced IL-12 in the spleen cells of naïve mice.
Y, Kaburaki, T, Fujimura, K, Kurata, K, Masuda, M, Toda, H, Yasueda, K, Chida, S, Kawarai, M, Sakaguchi   +8 more
openaire   +2 more sources

Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies

Veterinary Immunology and Immunopathology, 2001
In our previous study [Immunology 91 (1997) 161] using monoclonal antibodies (mAbs) specific to Cry j 1, a major allergen in Japanese cedar (Cryptomeria japonica) pollen, we identified five independent epitopes (EP-1-EP-5) on the molecule and found that EP-1 and EP-5 are the predominant allergic epitopes for humans and monkeys, respectively.
M, Sakaguchi, K, Masuda, M, Toda, S, Inouye, H, Yasueda, Y, Taniguchi, T, Nagoya, D J, DeBoer, H, Tsujimoto   +8 more
openaire   +2 more sources

Comparison of concentrations of Cry j 1 and Cry j 2 in diploid and triploid Japanese cedar (Cryptomeria japonica) pollen extracts

Allergy, 1997
Japanese cedar (Cryptomeria japonica) pollinosis has been a serious allergic disease in Japan. There are two kinds of Japanese cedar trees; the popular one is diploid, the less popular is triploid. These trees are not very different morphologically. However, the relative allergenicity of their pollens is unknown, although both major allergens, Cry j 1 ...
Y, Kondo, H, Ipsen, H, Løwenstein, A, Karpas, L S, Hsieh   +4 more
openaire   +2 more sources

IgE-reactivity to major Japanese cedar (Cryptomeria japonica) pollen allergens (Cry j 1 and Cry j 2) by ELISA in dogs with atopic dermatitis

Veterinary Immunology and Immunopathology, 2000
The present study investigated IgE-reactivity to two major Japanese cedar (Cryptomeria japonica, C. japonica) pollen allergens (Cry j 1 and Cry j 2) in dogs with atopic dermatitis by use of a fluorometric ELISA. The serum samples from 27 dogs that showed IgE-sensitivity to crude C.
K, Masuda, H, Tsujimoto, S, Fujiwara, K, Kurata, A, Hasegawa, Y, Taniguchi, K, Yamashita, H, Yasueda, D J, DeBoer, A L, de Weck, M, Sakaguchi   +10 more
openaire   +2 more sources

Preclinical evaluation of an immunotherapeutic peptide comprising 7 T-cell determinants of Cry j 1 and Cry j 2, the major Japanese cedar pollen allergens

Journal of Allergy and Clinical Immunology, 2001
Peptide immunotherapy is a new approach to treating allergic diseases, but a therapeutic peptide for Japanese cedar pollinosis has not yet been developed.The aim of this study is to prepare and preclinically evaluate a hybrid peptide comprising 7 T-cell determinants of Cry j 1 and Cry j 2, the major Japanese cedar pollen allergens.The recombinant ...
K, Hirahara, T, Tatsuta, T, Takatori, M, Ohtsuki, H, Kirinaka, J, Kawaguchi, N, Serizawa, Y, Taniguchi, S, Saito, M, Sakaguchi, S, Inouye, A, Shiraishi   +11 more
openaire   +2 more sources

Roles of major oligosaccharides on Cry j 1 in human immunoglobulin E and T cell responses

Clinical & Experimental Allergy, 2004
SummaryBackground We have demonstrated that carbohydrates in Cry j 1, the major allergen of Cryptomeria japonica pollen, play a major role in promoting Cry j 1‐specific Th2 response. However, little is known as to whether the carbohydrates directly participate in allergic responses.Objective We sought to determine whether Cry j 1‐related ...
M, Okano, Y, Kimura, K, Kino, Y, Michigami, S, Sakamoto, Y, Sugata, M, Maeda, F, Matsuda, M, Kimura, T, Ogawa, K, Nishizaki   +10 more
openaire   +2 more sources

Structural basis for epitopes sharing between group 1 allergens of cedar pollen, Jun a 1 and Cry j 1

Journal of Allergy and Clinical Immunology, 2004
Abstract Rationale Resolution of the 3D-structures and IgE epitopes of allergens may identify common or conserved features important for the biological activity of allergens. Methods Purified Jun a 1 was crystallized using the hanging-drop vapour-diffusion method at 277 K.
T. Midoro-Horiuti, D. Liu, M.A. White, E.W. Czerwinski, V. Mathura, C.H. Schein, W. Braun, M. Watanabe, T. Oka, A. Togawa, E.G. Brooks, R.M. Goldblum   +11 more
openaire   +1 more source

Reduction of Antigenicity of Cry j 1, a Major Allergen of Japanese Cedar Pollen, by Thermal Denaturation

Journal of Agricultural and Food Chemistry, 2009
The soluble aggregates of Cry j 1, a major allergen of Japanese cedar pollen, were formed without any coagulates during heat treatment at acidic pH 5, as shown in HPLC and SDS-PAGE patterns. A remarkable change in the CD spectrum was observed between native and heat-denatured Cry j 1 at a linear rate of 1 degrees C/min from 40 to 90 degrees C.
Rieko, Aoki, Akira, Saito, Masakatsu, Usui, Hiroyuki, Azakami, Akio, Kato   +4 more
openaire   +2 more sources

Cry j 1 isoforms derived from Cryptomeria japonica trees have different binding properties to monoclonal antibodies

Clinical & Experimental Allergy, 2004
SummaryBackground We identified five Cryptomeria japonica trees producing Cry j 1 isoforms that cannot be detected in a sandwich ELISA using two monoclonal antibodies, J1B01 and J1B07, suggesting that the binding affinity of these isoforms for both monoclonal antibodies is low.Objectives The binding properties of the Cry j 1 isoforms ...
Y, Goto, T, Kondo, T, Ide, H, Yasueda, N, Kuramoto, K, Yamamoto   +5 more
openaire   +2 more sources