Results 131 to 140 of about 99,841 (293)
Advanced Science, EarlyView.The AAA+ ATPase Valosin‐containing protein (VCP/p97) regulates protein homeostasis by unfolding ubiquitinated substrates. Here, we describe UTE‐156, a novel irreversible covalent inhibitor that modifies Cys522 in the D2 ATPase motor domain. Although its pharmacochemical limitations preclude immediate therapeutic use, UTE‐156 serves as a valuable ...
Daniela Tamayo‐Jaramillo +8 more
wiley +1 more source
A large-scale curated and filterable dataset for cryo-EM foundation model pre-training
Scientific DataCryo-electron microscopy (cryo-EM) is a transformative imaging technology that enables near-atomic resolution 3D reconstruction of target biomolecule, playing a critical role in structural biology and drug discovery.
Qihe Chen +7 more
doaj +1 more source
Advanced Science, EarlyView.ABSTRACT Extracellular vesicles (EVs) are nanoscale mediators of intercellular communication with diverse molecular cargoes that reflect their cell of origin. Advances in isolation, detection, and single‐particle analytics have revealed increasing molecular and functional heterogeneity, while exposing limitations in how EV identity and activity are ...
David J. Lundy +8 more
wiley +1 more source
Scientific DataThe advent of single-particle cryo-electron microscopy (cryo-EM) has brought forth a new era of structural biology, enabling the routine determination of large biological molecules and their complexes at atomic resolution.
Nabin Giri, Liguo Wang, Jianlin Cheng
doaj +1 more source
Graphene in cryo-EM specimen optimization
Current Opinion in Structural BiologySpecimen preparation is a critical but challenging step in high-resolution cryogenic electron microscopy (cryo-EM) structural analysis of macromolecules. In the past decade, graphene has gained much recognition as the supporting substrate to optimize cryo-EM specimen preparation.
Nan Liu, Hong-Wei Wang
openaire +2 more sources
Advanced Science, EarlyView.The 3K‐AP system forms infinite supramolecular fibrils that undergo controlled degradation into shorter fragments upon enzymatic hydrolysis. Multitechnique imaging reveals a stepwise bacteriolytic mechanism involving bacterial entrapment, disassembly into lethal constructs, and associated extracellular vesicle release, highlighting the dynamic ...
Sohini Chakraborty +21 more
wiley +1 more source
An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly
Nature CommunicationsMechanisms by which G-patch activators tune the processive multi-tasking ATP-dependent RNA helicase Prp43 (DHX15 in humans) to productively remodel diverse RNA:protein complexes remain elusive.
Daniela Portugal-Calisto +11 more
doaj +1 more source
ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid‐β Pathology
Advanced Science, EarlyView.This study identifies that ALKBH3‐driven m1A demethylation orchestrates Alzheimer's disease progression by disrupting mitochondrial and synaptic homeostasis. This epitranscriptomic mechanism suppresses PINK1‐mediated mitophagy via m1A erasure, leading to mitochondrial dysfunction, oxidative stress, elevated Aβ production, and impaired microglial ...
Yueyang Li +25 more
wiley +1 more source
Cryo-electron microscopy-based drug design
Frontiers in Molecular BiosciencesStructure-based drug design (SBDD) has gained popularity owing to its ability to develop more potent drugs compared to conventional drug-discovery methods.
Ecenur Cebi +5 more
doaj +1 more source