Results 121 to 130 of about 177,867 (249)
Small, EarlyView.In this work, a femtosecond (fs) laser processing strategy is demonstrated for rapid and targeted fabrication of 3D sub‐100‐µm microstructures on resin‐embedded cells. The fabricated array of sub‐100‐µm pillars (each loaded with flow‐sorted T‐cell subsets) is arranged in a half‐grid geometry to enable efficient use of the scarce biological sample and ...
Aristide Djoulde +6 more
wiley +1 more source
Small, EarlyView.Aerosol Jet Printing (AJP), coupled with a novel in situ monitoring method, enabled the controlled fabrication of aligned and crumpled Ti3C2Tx MXenes for diverse applications. Demonstrated across multiple 2D materials, this scalable strategy stablishes flake morphology as a controllable design parameter for engineering application‐specific material ...
Javier Gutierrez‐Gonzalez +18 more
wiley +1 more source
In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography
, 2020 Electron cryo-tomography (cryo-ET) is a technique that allows the investigation of intact macromolecular complexes while they are in their cellular milieu.
Chreifi, Georges +15 more
core +1 more source
3D Reconstruction of Nanoparticle Distribution in Tumor Spheroids With Volume Electron Microscopy
Small, EarlyView.Understanding where nanomaterials accumulate within cells is essential for revealing their biological fate and activity. We present a quantitative imaging framework to map nanomaterials and cellular structures in three dimensions. Applied to nanoparticle‐containing tumor spheroids, the approach reveals substantial cell‐to‐cell differences in uptake ...
Davide Bottone +11 more
wiley +1 more source
Focused ion beam milling of vitreous water: prospects for an alternative to cryo-ultramicrotomy of frozen-hydrated biological samples [PDF]
, 2020 Summary The feasibility of using a focused ion beam (FIB) for the purpose of thinning vitreously frozen biological specimens for transmission electron microscopy (TEM) was explored.
core
SusMat, EarlyView.This graphical ToC illustrates a tubular‐inspired detoxifier (SDCMs–DS) that halts acute kidney injury progression. Inspired by proximal tubular epithelial cells (PTECs), the biomimetic design combines dissociation‐adsorption clearance of protein‐bound uremic toxins (PBUTs) with antioxidant protection. During hemoperfusion, danshensu (DS) released from
Yilin Wang +12 more
wiley +1 more source
Diabetes, Obesity and Metabolism, EarlyView.ABSTRACT Aim To develop a next‐generation, longer‐acting, more‐clinical‐benefits agonist to achieve sustained efficacy with improved tolerability. Methods CT130 was designed from semaglutide. Binding affinity was validated via molecular dynamics and cellular assays.
Wei Ding +4 more
wiley +1 more source
Angewandte Chemie, Volume 138, Issue 44, 26 October 2026.We developed a fast, aqueous one‐pot synthesis for PEDOT:PSTFSI, combining sequential reversible addition–fragmentation chain‐transfer (RAFT) and oxidative polymerization. This method eliminates toxic solvents and purification steps, achieving full monomer conversion in under 3 h.
Fantine Negny +6 more
wiley +2 more sources
iFLM-guided Cryo-FIB milling v1
Defective mitochondrial quality control in response to loss of mitochondrial membrane polarization is implicated in Parkinson’s disease by mutations in PINK1 and PRKN. Application of in situ cryo-electron tomography (cryo-ET) made it possible to visualize the consequences of mitochondrial depolarization at higher resolution than heretofore attainable.
openaire +1 more source
In-house manufactured holder for cryo-stage annular milling of atom probe specimens.
, 2020 (A) Holder mounted on the cryo-stage. (B) Schematic view of the holder within the dual-beam FIB.
Nicolás Alfonso Rivas (8313426) +8 more
core +1 more source