Results 141 to 150 of about 177,867 (249)

Cryo-FIB milling improves microED data and structures [PDF]

Acta Crystallographica Section A Foundations and Advances, 2021
openaire   +1 more source

Thinner is not always better: Optimizing cryo-lamellae for subtomogram averaging [PDF]

Cryo-electron tomography (cryo-ET) is a powerful method to elucidate subcellular architecture and to structurally analyze biomolecules in situ by subtomogram averaging, yet data quality critically depends on specimen thickness.
Hummer, G. ; https://orcid.org/, Kreysing, J. ; https://orcid.org/, Cruz-León, S. ; https://orcid.org/, Welsch, S. ; https://orcid.org/, Tuijtel, M. ; https://orcid.org/, Turoňová, B. ; https://orcid.org/, Beck, M. ; https://orcid.org/   +6 more
core   +1 more source

Depth-resolved analysis reveals target-dependent cryo-FIB damage profiles

bioRxiv
Cryogenic focused ion beam (cryo-FIB) milling enables cellular cryo-electron tomography but can compromise high-resolution structural information near milled surfaces.
Robert M. Nwokonko, M. Z. Ǫayyum, D. Karia, Vishal Maingi, Amrita Rai, Y. Sheng, Syed Asfarul Haque, Christopher Thompson, Sanja Sviben, Haruaki Yanagisawa, J. M. de la Rosa-Trevín, Ravi C. Kalathur, Georgios Skiniotis, Abhay Kotecha   +13 more
semanticscholar   +1 more source

Cryo-FIB and Transmission Electron Microscopy of Cryoformed Metals

Microscopy and Microanalysis, 2022
Ana Luisa Terasawa Senra, Daniel E Huber, Ricardo S Namur, Antonio J Ramirez, Osvaldo M Cintho   +4 more
openaire   +1 more source

EM grid seeding for cryo-FIB milling v1

Defective mitochondrial quality control in response to loss of mitochondrial membrane polarization is implicated in Parkinson’s disease by mutations in PINK1 and PRKN. Application of in situ cryo-electron tomography (cryo-ET) made it possible to visualize the consequences of mitochondrial depolarization at higher resolution than heretofore attainable.
openaire   +1 more source

The PRIMA Thesaurus for Materials Science and Engineering

Advanced Engineering Materials, Volume 28, Issue 20, 21 October 2026.
The PRIMA Thesaurus is a structured vocabulary designed to improve how materials science data is described and shared. Developed with input from multiple experts, it enables clear documentation of research workflows, data exchange, and reuse across platforms.
Rossella Aversa, Alexey Boubnov, Dario De Angelis, Catriona Eschke, Maximilian Kabbe, Felix Kraus, Mirco Panighel, Richard Thelen, Davide Valentinis   +8 more
wiley   +1 more source

Thickness- and quality-controlled fabrication of fluorescence-targeted frozen-hydrated lamellae

Cell Reports: Methods
Summary: Cryogenic focused ion beam (FIB) milling is essential for fabricating thin lamella-shaped samples out of frozen-hydrated cells for high-resolution structure determination.
Daan B. Boltje, Radim Skoupý, Clémence Taisne, Wiel H. Evers, Arjen J. Jakobi, Jacob P. Hoogenboom   +5 more
doaj   +1 more source

Imaging optimization of FIB cross sections for reliable porosity analysis of PEMFC cathode catalyst layers

Applied Microscopy
Focused ion beam–scanning electron microscopy (FIB–SEM) was used to investigate the effects of acceleration voltage, beam current, and cryogenic conditions on the cross-sectional preparation of proton exchange membrane fuel cell (PEMFC) cathode catalyst ...
Ji Hee Kwon, Jinnyeong Jo, Byeong-Seon An   +2 more
doaj   +1 more source

Cryo-lift-out Technique for Cryo-electron Tomography of Tissue Samples

Shengwu huaxue yu shengwu wuli jinzhan
Cryo-electron tomography (cryo-ET) enables the determination of high-resolution three-dimensional structures of macromolecular complexes within cells in a near-physiological state, providing crucial structural insights into fundamental life processes ...
QIN Chang-Dong, GUO Qiang, GAO Ning
doaj   +1 more source

Whole cryo-FIB lamella acquisition at high magnification with rectangular beam montage cryo-electron tomography

bioRxiv
The visualisation of large biological samples in cryo-electron tomography requires both few-nanometre resolution and a wide, ideally >10 µm, field of view.
Hamish G. Brown, S. Valimehr, Denver Styczynski, M. Mudaliyar, Srujan Shetty Kundapura, Matthew D. Johnson, D. Ghosal, E. Hanssen   +7 more
semanticscholar   +1 more source