Results 131 to 140 of about 17,043 (166)
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Automated Identification of Filaments in Cryoelectron Microscopy Images
Journal of Structural Biology, 2001Since the foundation for the three-dimensional image reconstruction of helical objects from electron micrographs was laid more than 30 years ago, there have been sustained developments in specimen preparation, data acquisition, image analysis, and interpretation of results.
Bridget Carragher +2 more
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Cryoelectron microscopy of macromolecular complexes
Biology of the Cell, 1994Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X‐ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies.
R H, Wade, E A, Hewat
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Cryoelectron Microscopy of Fission Yeast
Cold Spring Harbor Protocols, 2017Fission yeast cells can be prepared for electron microscopy (EM) in the frozen-hydrated state. This eliminates the requirement for dehydration and heavy metal staining when preparing samples for EM. As with room temperature imaging, however, the yeast must be sectioned to make them thin enough for transmission of the electron beam.
Mary K, Morphew +2 more
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Cryoelectron microscopy of vitrified Sendai virions
Journal of Virological Methods, 1988Morphology of vitrified Sendai virions was studied by transmission type electron microscopy. Almost all the virions appeared to be completely spherical, although their diameters differed. A possibly continuously long nucleocapsid was seen running helically in an envelope. Spikes were seen on the virion surfaces.
Y, Hosaka, T, Watabe
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Cryoelectron Microscopy of Liposomes
2005A thin aqueous film of suspended lipid vesicles?micelles is the object of choice for vitrification and subsequent study by cryoelectron microscopy. Just prior to vitrification, a thin film (compare with a soap film) is vulnerable to heat and mass exchange.
Peter M, Frederik, D H W, Hubert
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Virus structures revealed by advanced cryoelectron microscopy methods
Structure, 2023Before the resolution revolution, cryoelectron microscopy (cryo-EM) single-particle analysis (SPA) already achieved resolutions beyond 4 Å for certain icosahedral viruses, enabling ab initio atomic model building of these viruses. As the only samples that achieved such high resolution at that time, cryo-EM method development was closely intertwined ...
Dongjie, Zhu +2 more
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Cryoelectron Microscopy of Icosahedral Virus Particles
2007With the rapid progresses in both instrumentation and computing, it is increasingly straightforward and routine to determine the structures of icosahedral viruses to subnanometer resolutions (6-10 A) by cryoelectron microscopy and image reconstruction.
Wen, Jiang, Wah, Chiu
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Cryoelectron microscopy with elemental sensitivity
StructureIn a recent issue of Nature Methods, Pfeil-Gardiner et al. (2024)1 combine electron energy-loss spectroscopy and single-particle cryoelectron microscopy to allow the spatially resolved imaging of the elemental composition of macromolecules.
Hannah, Ochner, Tanmay A M, Bharat
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Structure of bluetongue virus particles by cryoelectron microscopy
Journal of Structural Biology, 1992The structure of the bluetongue virus (BTV) particle, determined by cryoelectron microscopy and image analysis, reveals a well-ordered outer shell which differs markedly from other known Reoviridae. The inner shell is known to have an icosahedral structure with 260 triangular spikes of VP7 trimers arranged on a T = 13,l lattice. The outer shell is seen
E A, Hewat, T F, Booth, P, Roy
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