Results 101 to 110 of about 79,865 (300)

Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari, Sophie J. Wensley, Malak A. Ammar, Avinash Srivastava, Changhavi Ragurajan, Jenish Patel, Natalie C. Elliott, Alice C. Hodgson‐Casson, Mariano Olivera Fedi, Michael Anbar, Peter M. O'Callaghan, Bernie Sweeney   +11 more
wiley   +1 more source

Quarantine and cryopreservation [PDF]

Human Reproduction, 1997
G, Bahadur, R S, Tedder
openaire   +2 more sources

Integrating chemo‐mechanical cues in nano‐microscale environments for stem cell regulation

BMEMat, EarlyView.
Nano/microscale control strategies, including nano/microscale materials, ligand spacings, and cell encapsulating gels, cell mechanics, integrin signaling, and biochemical cues to direct stem cell fate, tissue regeneration, and organoid engineering, enabling advanced biomedical applications through mechanoregulation at cell‐material interfaces ...
Rajendra K. Singh, Varsha Sagar, Amal George Kurian, Shreyas Kumar Jain, Hye Sung Kim, Hae‐Won Kim   +5 more
wiley   +1 more source

Latent class analysis of Chinese healthcare providers’ attitudes towards oocyte cryopreservation: a cross-sectional study

BMJ Open
Objectives The present study was designed to examine the attitudes towards oocyte cryopreservation among healthcare providers working in hospitals across specialties and potential influencing factors.Design A cross-sectional study.Setting The ...
Jingjing Lu, Xuezi Tian, Zhaochen Wang
doaj   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

A review on the functional roles of trehalose during cryopreservation of small ruminant semen

Frontiers in Veterinary Science
Sperm cryopreservation is an approach to preserve sperm cells in liquid nitrogen or other cryogenic media for future use in assisted reproductive technologies, such as in vitro fertilization or artificial insemination.
Baoyu Jia, Larbi Allai, Larbi Allai, Larbi Allai, Chunyan Li, Chunyan Li, Chunyan Li, Jiachong Liang, Jiachong Liang, Jiachong Liang, Chunrong Lv, Chunrong Lv, Chunrong Lv, Guoquan Wu, Guoquan Wu, Guoquan Wu, Guobo Quan, Guobo Quan, Guobo Quan   +18 more
doaj   +1 more source

Benchmarking of Fluorescent Proteins in Trichoderma reesei Using Flow Cytometry

Cytometry Part A, EarlyView.
ABSTRACT Fungi are the subject of extensive research due to their significant impact on human life. However, the development of tools based on fluorescent proteins has been slow in these species. Trichoderma reesei, a filamentous fungus known for its high secretion capability, shows great potential for producing heterologous proteins.
Métivier Killian, Bidard Frédérique, Jourdier Etienne, Mathis Hugues   +3 more
wiley   +1 more source

Recent advances of the egg\'s freezing: An overview

Journal of Mazandaran University of Medical Sciences, 2018
It is now more than three decades since the first live birth from oocyte cryopreservation. At present, oocyte cryopreservation has become a major component of Assisted Reproductive technologies (ART).
Faranak Aghaz, Mozafar Khazaei
doaj  

Evaluation of a multiple myeloma synthetic cell mimic as an assay control for flow cytometry

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract The reliability of clinical flow cytometry assays hinges on the availability of robust assay controls that accurately reflect representative samples. Widely used conventional assay controls, such as cell lines, often fail to replicate the phenotypical and biophysical properties of representative primary samples resulting in gating variability ...
Dhong Hyun Lee, Mike Liu, Kanwal Palla, Subhanip Biswas, Swetha Pratyusha Gunturu, Brian Kerfs, Kristine Valenteros, Sarah Kotanchiyev, Shari Kaiser, Justine Dell'Aringa   +9 more
wiley   +1 more source