Results 121 to 130 of about 159,718 (398)

The Current Trends in Using Nanoparticles, Liposomes, and Exosomes for Semen Cryopreservation

Animals, 2020
Simple Summary Long-term preservation of semen is a pivotal step for artificial insemination in most farm animal species, but it is associated with cellular insults at the cell membrane and cytoskeleton level as well as the generation of reactive oxygen ...
I. Saadeldin, W. Khalil, M. Alharbi, Seok Hee Lee   +3 more
semanticscholar   +1 more source

A fluorescent MTP‐based Detection Platform for Hydrogen Peroxide, Glucose, and Lactate

Analysis &Sensing, Accepted Article.
Hydrogen Peroxide (H2O2) is an important small metabolite often quantified with commercially available multi‐step fluorescence‐based assays. We developed a new microtiter plate (MTP) based platform that allows a rapid, one‐step assay with a ratiometric readout function.
John J. Galligan, Antje J. Baeumner, Axel Duerkop   +2 more
wiley   +1 more source

Scalable, High‐Density Expansion of Human Mesenchymal Stem Cells on Microcarriers Using the Bach Impeller in Stirred‐Tank Reactors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT This paper describes the results of process developmental experiments to achieve higher cell densities in the manufacturing of hMSCs using the novel Bach impeller in a stirred‐tank bioreactor. Engineering experiments have previously shown that the Bach impeller represents an efficient mixing device that suspends particles in fluids at low ...
Tom A. Wyrobnik, Laia Miranda, Alan Lam, Steve Oh, Andrea Ducci, Martina Micheletti   +5 more
wiley   +1 more source

Strategies to Minimize Various Stress-Related Freeze-Thaw Damages During Conventional Cryopreservation of Mammalian Spermatozoa.

Biopreservation and Biobanking, 2019
The aim of the article is to report a review on different sperm cryopreservation techniques, various stress-related freeze-thaw damages altering sperm structure and function during conventional cryopreservation, and strategies to minimize these stresses.
Abhishek Kumar, Janeshwar Prasad, N. Srivastava, S. Ghosh   +3 more
semanticscholar   +1 more source

Optimizing biophysical properties of cellular niches to enhance stem cell‐derived extracellular vesicle function in musculoskeletal regeneration

BMEMat, EarlyView.
Extracellular vesicles (EVs) derived from stem cells show promise for applications in regenerative medicine, but their scalability and yield remain challenges. This review explores the approaches for biophysical modulations within cell niches on EV properties, discusses the current clinical application of EVs, and provides an outlook on the existing ...
Yang Xu, Chung Yin Matthew Cheung, Liling Liu, Hoi Pan Harry Cheung, Kam Yu Tam, Dai Fei Elmer Ker, Sarah H. Cartmell, Chuanbin Mao, Zhiyong Zhang, Dan Michelle Wang   +9 more
wiley   +1 more source

Development of Islet Cryopreservation for Type 1 Diabetes Cell Therapy

Zhileng xuebao, 2021
In recent years, cell therapy technology for type 1 diabetes has advanced rapidly, but the lack of donor pancreatic islets and the time and space mismatch between donor and recipient limits its clinical application. Cryopreservation technology provides a
Han Hengxin, Zhan Taijie, Cui Mengdong, Yang Jiamin, Chen Liang, Xu Yi   +5 more
doaj  

Cryopreservation of Human Ovarian Tissue: A Review

Transfusion Medicine and Hemotherapy, 2019
Background: Cryopreservation of human ovarian tissue has been increasingly applied worldwide to safeguard fertility in cancer patients, notably in young girls and women who cannot delay the onset of their treatment.
Ellen Cristina Rivas Leonel, C. M. Lucci, C. Amorim   +2 more
semanticscholar   +1 more source

Cryopreservation practices in clinical and preclinical iPSC‐based cell therapies: Current challenges and future directions

Biotechnology Progress, EarlyView.
Abstract Induced pluripotent stem cells (iPSCs) offer significant therapeutic potential, but cryopreservation challenges, particularly the reliance on cytotoxic Dimethyl Sulfoxide (Me2SO), hinder their clinical application. This review examines current cryopreservation practices in clinical and preclinical iPSC‐based therapies, highlighting the ...
Michael Dobruskin, Geoffrey Toner, Ronald Kander   +2 more
wiley   +1 more source

Cryopreservation techniques and their application in vegetatively propagated crop plants in Finland

Agricultural and Food Science, 2008
Cryopreservation protocols have been introduced as techniques for germplasm preservation of vegetatively propagated horticultural and staple food crops. In Finland, cryopreservation has been studied since 1990’s, beginning with cryopreservation of forest
A. NUKARI, M. UOSUKAINEN, V-M. ROKKA
doaj  

Controlled ovarian stimulation in cancer patients under 18 years old; a case series

Journal of Ovarian Research
Background Fertility preservation for adolescent pubescent girls is a concern of the healthcare system and parents. Oocyte cryopreservation is regarded as a standard medical intervention for patients with a minimum age of 18 years. Evidence suggests that
Azar Yahyaei, Mahdieh Moridi, Firouzeh Ghaffari   +2 more
doaj   +1 more source