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Cryopreservation of Organoids

Cryoletters, 2023
Organoids represent indispensable opportunities for biomedicine, including drug discovery, cancer biology, regenerative and personalised medicine or tissue and organ transplantation. However, the lack of optimised preservation strategies limits the wide use of organoids in research or clinical fields.
O, Rogulska, J, Havelkova, Y, Petrenko
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Cryopreserved bioprostheses

IEEE Engineering in Medicine and Biology Magazine, 1989
The process of cryopreservation is described. Three types of tissue are then considered: allograft heart valves, allograft veins and the musculoskeletal soft tissues of the knee. The problems with each of these tissue types are examined.
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Embryo Cryopreservation

Seminars in Reproductive Medicine, 1998
Cryopreservation stands as an ongoing evolution in the field of assisted reproductive technologies. Face with increasing numbers of fertilized oocytes and early embryos, cryopreservation avails the ART program of a useful means to preserve embryos for future use without exposing patients to the risks of multiple pregnancies.
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Cryopreservation of Hepatocytes

2014
The use of cryopreserved hepatocytes has increased in the last decade due to the improvement of the freezing and thawing methods, and has even achieved acceptance by the US Food and Drug Administration for use in drug metabolizing enzyme induction studies.
Nicola J, Hewitt, Albert P, Li
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Cryopreservation of cartilage

International Orthopaedics, 1990
We have investigated the viability and function of cells in cartilage slices after various methods of preservation, and have examined the viability of cells by measuring the incorporation of Na2(35)SO4 at different concentrations, temperatures and times of exposure to cryopreservatives.
N, Kawabe, M, Yoshinao
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Cryopreservation of Cells

Current Protocols in Immunology, 1997
AbstractLaboratories interested in working with in vitro cultured cells must be able to freeze and thaw cell lines. Such capabilities are necessary to maintain the constancy of the cells since prolonged culture may result in phenotypic and genotypic changes. In addition, cell freezing minimizes problems with contamination as well as the overall expense
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Cryopreservation of Eggs

2014
Oocyte cryopreservation is playing an increasingly important role in the field of human infertility treatment. The ability to store viable oocytes for later use has given many women the option to delay childbearing in order to pursue other ventures in life, without the concern of losing the opportunity to have a family.
Zsolt Peter, Nagy   +4 more
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Cryopreservation of Hybridomas

2003
Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
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Cryopreservation of parasites

Experientia, 1988
In this review, advances in cryopreservation of helminth parasites are reported. Our own studies demonstrate that metacestodes of Echinococcus multilocularis can be maintained in a viable state for at least 1-2 years by appropriate deep-freezing and storage in liquid nitrogen.
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Cryopreservation of helminths

Parasitology Today, 1985
Department of Opthalmology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29464, USA Fig. I. The effect of cooling rate on cell survival. Each cell type has its own optimum cooling rate (A). At slower rates the cells are injured by solution effects.
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