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Technologies for Vitrification Based Cryopreservation
Bioengineering, 2023 Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy, tissue ...
Mohammad Amini, James D. Benson
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Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges
Animals, 2022 Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro +4 more
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浙江大学学报. 农业与生命科学版, 2020 Antifreeze proteins (AFPs), a class of antifreeze substances, were initially found to bind to ice crystals in vivo, thereby contributing to inhibition of the growth of ice crystals that enabled polar fish to survive at the temperatures below freezing ...
HENG Nuo +8 more
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A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. [PDF]
PLoS ONE, 2014 Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low.
Keitaro Imaizumi +8 more
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Cryopreservation of Human Mucosal Leukocytes. [PDF]
PLoS ONE, 2016 BACKGROUND:Understanding how leukocytes in the cervicovaginal and colorectal mucosae respond to pathogens, and how medical interventions affect these responses, is important for developing better tools to prevent HIV and other sexually transmitted ...
Sean M Hughes +19 more
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An efficient, economical slow-freezing method for large-scale human embryonic stem cell banking [PDF]
, 2012 Human embryonic stem cells (hESCs) are one of the most interesting cell types for tissue engineering, cell therapy, basic scientific research, and drug screening. Fast advancement in these areas requires the availability of large amounts of safe and well-
Cornelissen, Maria +3 more
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, 2020 Cryopreservation has been successfully used in the banking and maintenance of cultures of microorganisms, from bacteria to yeasts, since the onset of cryobiology. Biobanking of marine biological resources is crucial for development of scientific knowledge as researchers rely on guaranteed access to reliable, stable resources. Culture collections play a
Paredes Rosendo, Estefanía +4 more
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Frontiers in Bioengineering and Biotechnology, 2020 Human induced pluripotent stem cells (hiPSCs) are an important cell source for regenerative medicine products. Effective methods of preservation are critical to their clinical and commercial applications.
Rui Li +3 more
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Plants, 2021 Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should
Fatiha Bradaï, Carolina Sánchez-Romero
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Step by step optimization of a sperm cryopreservation protocol for spotted wolffish (Anarhichas minor Olafsen, 1772) [PDF]
, 2020 Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, low sperm volume with relatively low cell concentration and the lack of gametes synchronization (simultaneous availability of mature eggs and ...
Beirão, J. +3 more
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