Results 111 to 120 of about 32,701 (270)

Human Gallbladder Cancer Organoids

Cell Proliferation, EarlyView.
This guideline provides standardized requirements and quality control measures for human gallbladder cancer organoids, supporting their reliable application in research and precision medicine. It aims to promote methodological consistency, facilitate international collaboration, and accelerate translational research and clinical implementation.
Dongxi Xiang, Ting Gong, Yinjie Zhu, Bin Jia, Chen Zhang, Ning Tang, Weiping Wang, Yalong Wang, Laiping Zhong, Yanmei Zou, Jian Zhang, Zunqiang Zhou, Ligang Xing, Changchun Zhou, Dongyuan Zhu, Zengjun Liu, Gang Chen, Xiaoguang Wang, Zhan Wang, Aina He, Yonggang Wang, Zebing Liu, Jiabei Wang, Feng Xu, Ganglong Gao, Xiaonan Kang, Shuai Gong, Xianming Kong, Chunyan Dong, Aijin Ma, Gengming Niu, Guiying Wei, Hongling Zhao, Xiongxiong Lu, Jie Cao, Tongbiao Zhao, Zizhen Zhang, Yingbin Liu   +37 more
wiley   +1 more source

Prolonged storage reduces viability of Peptacetobacter (Clostridium) hiranonis and core intestinal bacteria in fecal microbiota transplantation preparations for dogs

Frontiers in Microbiology
IntroductionFecal microbiota transplantation (FMT) has been described useful as an adjunct treatment for chronic enteropathy in dogs. Different protocols can be used to prepare and store FMT preparations, however, the effect of these methods on microbial
Bruna Correa Lopes, Jonathan Turck, M. Katherine Tolbert, Paula R. Giaretta, Jan S. Suchodolski, Rachel Pilla, Rachel Pilla   +6 more
doaj   +1 more source

Measurement of Water-Cryoprotectant Mutual Diffusivity in Micro-Scale Biological Tissues at Subzero Temperatures

, 2009
Water-cryoprotectant mutual diffusivity (D¯) in biological tissues at subzero temperatures is of critical importance for optimizing tissue or organ cryopreservation procedures. Currently, there exist no attempts to measure D¯ at subzero temperatures.
Hongbin Ma, John K. Critser, Xu Han, Yang Liu, James D. Benson   +4 more
core   +1 more source

Unraveling the active site cover of coproheme decarboxylase from Listeria monocytogenes

The FEBS Journal, EarlyView.
During heme biosynthesis in Gram‐positive bacteria, coproheme decarboxylase (ChdC) catalyzes the conversion of four‐propionate substrate coproheme to the two propionate product heme b. Its active site is universally covered by a flexible linking loop. This study identifies an important histidine residue, which stabilizes the loop in a ChdC homolog.
Nikolaus Falb, Dominik Hörtnagel, Chris Oostenbrink, Paul G. Furtmüller, Stefan Hofbauer   +4 more
wiley   +1 more source

Characterization of the vitrification parameters for oviduct aggregates in Camelus dromedarius

Frontiers in Veterinary Science
IntroductionCamel oviductal isthmus aggregates provide a novel and promising model for studying sperm attachment and longevity, offering a potential alternative for short-to mid-term sperm preservation and transport under non-cryogenic conditions ...
Mohamed M. M. El-Sokary, Mohamed M. M. El-Sokary, Hamad A. Albreiki, Seham F. Shehata, Karima Gh. M. Mahmoud   +4 more
doaj   +1 more source

Cryoprotectant toxicity studies on Crassostrea angulata sperm

, 2015
International audienceThe preservation of genetic material of both improved stocks and of original population is very important for the oyster aquaculture industry to avoid potential impacts from epidemic diseases and natural disasters.
Matias, Domitília, Suquet, Marc, Cabrita, Elsa, Joaquim, Sandra, Riesco, Marta F., Martinez-Páramo, Sonia, Labbé, Catherine, Félix, Francisca   +7 more
core   +1 more source

Structural insights into θ‐type carbonic anhydrases 3 and 4: Tuning the directionality of CO2 hydration in a diatom

The FEBS Journal, EarlyView.
θ‐CA3 from a marine diatom has an N‐terminal transmembrane helix and two homologous catalytic domains. The enzyme functions as a dimer, with active sites formed near the dimer interface. A bicarbonate ion binds on the symmetry axis of the dimer and forms hydrogen bonds with both monomers, thereby activating θ‐CA3 and tuning CO2 hydration activity ...
Hiroto Negoro, Atsuki Ohsawa, Ginga Shimakawa, Hideaki Tanaka, Yusuke Matsuda, Genji Kurisu   +5 more
wiley   +1 more source

Design of Ready-to-Use “Ball-in-Ball” Staphylococcus aureus Microsphere Based on Novel Cryoprotectant and Drop Freeze-Drying Technology: Effective Preservation and Application

Foods
Staphylococcus aureus (S. aureus) poses a significant threat to public health and safety, and enhancing the monitoring of S. aureus in food is essential to curb and prevent foodborne transmission.
Zile Wang, Dongdong Chen, Xiaomei Zheng, Yuqing Li, Shaoqian Jiang, Yanfei Chen, Jingjian Jia, Libo Yu, Tao Peng   +8 more
doaj   +1 more source

Defining the role of αC helix interactions in the activation of the integrin αI domain

The FEBS Journal, EarlyView.
The α2I domain of the α2β1 integrin contains a distinctive αC helix and a conserved Arg288‐Glu318 ion pair that stabilizes the closed state of the domain. Disruption of this ion pair in the activated α2I variant unwinds the αC helix, which allows interaction with additional collagen‐binding sites increasing collagen binding.
Liisa Pösö, Tomi T. Airenne, Jarmo Käpylä, Hongmin Tu, Eva Bligt‐Lindén, Vimal Parkash, Jyrki Heino, Tiina A. Salminen   +7 more
wiley   +1 more source

Kriopengawetan telur Lernaer cyprinacea dengan menggunakan Cellvation sebagai bahan 'Cryoprotectant'

, 1994
Di dalam kajian ini, tiga teknik kriopengawetan telah dijalankan ke atas telur Lernaea cyprinacea sejenis parasit kumpulan krustasia dengan menggunakan Cellvatiori? sebagai bahan "cryoprotectant'
Elias Abdullah
core