Results 111 to 120 of about 29,937 (163)
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Microwave properties of cryoprotectants
Cryobiology, 1978Abstract The values of the dielectric constant and of the loss tangent for pure samples of DMSO, ethylene glycol, and glycerol were determined over the temperature range of + 15 to −70 °C. An operating frequency range of 1.45 to 1.55 GHz was used, allowing direct application of the results of both 0.915 and 2.450 GHz studies.
J D, Macklis, F D, Ketterer
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Cryoprotectant toxicity and cryoprotectant toxicity reduction: In search of molecular mechanisms
Cryobiology, 1989Cryoprotectant toxicity is a fundamental obstacle to the full potential of artificial cryoprotection, yet it remains in general a poorly understood phenomenon. Unfortunately, most relevant biochemical studies to date have not met the basic criteria required for demonstrating mechanisms of toxicity.
G M, Fahy +4 more
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Unloading of cryoprotectants from cryoprotectant-loaded cells on a microfluidic platform
Biomedical Microdevices, 2017In this paper, a multistep dilution-filtration microdevice (MDFD) is developed for unloading cryoprotectants from cryoprotectant-loaded cells. The MDFD contained a diluent producing region, a dilution-filtration execution region, and a filtrate collection region.
Yufeng, Kang +6 more
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Animal Reproduction Science, 2015
The present study investigates the effects of five cryoprotectants (CPAs) and cryoprotectant combinations on the post-thaw total motility, progressive motility, viability, mitochondrial membrane potential and acrosome integrity in stallion spermatozoa. In Experiment 1, the objective was to compare the impact of different concentrations (2.5%, 3.5% and ...
Zhuangyuan Wu +9 more
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The present study investigates the effects of five cryoprotectants (CPAs) and cryoprotectant combinations on the post-thaw total motility, progressive motility, viability, mitochondrial membrane potential and acrosome integrity in stallion spermatozoa. In Experiment 1, the objective was to compare the impact of different concentrations (2.5%, 3.5% and ...
Zhuangyuan Wu +9 more
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Development and Application of Cryoprotectants
2018Cryopreservation involves the preservation of biological materials, including cells, embryos, tissues, and organs, at ultra-low temperatures (in a state of suspended animation), for a long period of time, and in a way that allows them to be restored whenever required.
Robin, Rajan, Kazuaki, Matsumura
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Cryoprotectant toxicity neutralization
Cryobiology, 2010Cryoprotectant toxicity is a fundamental limiting factor for the successful cryopreservation of living systems by both freezing and vitrification, and the ability to negate it would be attractive. Past attempts to demonstrate "cryoprotectant toxicity neutralization" (CTN) have had many ups and downs. First convincingly introduced by Baxter and Lathe in
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The use of perdeuteropolyethylpropylene (d-PEP) as a cryoprotectant for neutron studies of molecular organic solids is demonstrated by the solution of the structure of L-(--)-ephedrine hemihydrate from neutron powder diffraction data on a sample ...
Mikkel Jørgensen, Bente Lebech
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Cryoprotective properties of vasoconstriction
Cryobiology, 1974Abstract In standardized freezing experiments made on depilated rat skin, it was found that vasconstriction gives some cryoprotection, but, only if tissue temperatures do not drop below −30 °C. It is suggested that vasoconstriction, which is a well-known sequel to more moderate drops in tissue temperature, is in fact a physiological temperature ...
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Methanol as a cryoprotectant for equine embryos
Theriogenology, 2004Equine embryos (n=43) were recovered nonsurgically 7-8 days after ovulation and randomly assigned to be cryopreserved in one of two cryoprotectants: 48% (15M) methanol (n=22) or 10% (136 M) glycerol (n=21). Embryos (300-1000 microm) were measured at five intervals after exposure to glycerol (0, 2, 5, 10 and 15 min) or methanol (0, 15, 35, 75 and 10 min)
L D, Bass +5 more
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New cryoprotectant for cryorefractive surgery
Journal of Cataract and Refractive Surgery, 1991Cryorefractive surgeries, keratomileusis, keratophakia, and epikeratophakia cause destruction of keratocytes, which may result in postoperative corneal haze. We examined the effects of two cryoprotectants on keratocyte survival following freeze injury. We compared the ability of CPTES and the standard cryoprotectant KM-26 to prevent keratocyte death by
K, Kratz-Owens +2 more
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