The Natural Cryoprotectant Honey for Fertility Cryopreservation
Honey is a mixture of 25 sugars with other bioactive substances (i.e., organic acids, enzymes, antioxidants, and vitamins) and has been known as a highly nutritious functional food. Traditionally, it has been widely used in medicinal applications to cure
Faryal Farooq Cheepa +2 more
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Cryopreservation of common carp sperm [PDF]
Experiments were carried out to investigate the effect of five extenders (sucrose, glucose, fructose, KCl and a saline carp sperm extender) and two cryoprotectants (dimethyl-sulfoxide (DMSO) and methanol) on the cryopreservation of common carp sperm ...
Urbányi, Béla +5 more
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Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen [PDF]
The key role of a cryoprotectant is to minimize the chemical and physical stress which occurs during cooling, freezing and thawing of semen. The difference between the cryoprotectant (CPA) occurs in their permeability coefficient and the structural model
T. M. Kanimozhi +3 more
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Defining the optimal cryoprotectant and concentration for cryopreservation of limbal stem cells [PDF]
Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure.
Lako, Majlinda +4 more
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Cryoprotectant-Free Freezing of Cells Using Liquid Marbles Filled with Hydrogel
Cryopreservation without cryoprotectant remains a significant challenge for the re-establishment of cell culture after freeze–thaw. Thus, finding an alternative and a simple cryopreservation method is necessary.
Ian Cock (6060068) +11 more
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Viability of dourado embryos cooled in different cryoprotectant solutions [PDF]
The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested.
Jayme Aparecido Povh +7 more
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Cryoprotectant agent toxicity in porcine articular chondrocytes.
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective ...
Forbes, J. F. +5 more
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Cryoprotectant combination ethylene glycol and propanediol on mice blastocyst viability post vitrification [PDF]
The best method to store embryo is vitrification using cryoprotectant, but so far, the viability of the embryo after warming process is decreasing. This objective of this research was to know the effect combination ethylene glycol and propanediol as ...
A. Kresna +2 more
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Cryoprotectant kinetic analysis of a human articular cartilage vitrification protocol
We recently published a protocol to vitrify human articular cartilage and a method of cryoprotectant removal in preparation for transplantation. The current study’s goal was to perform a cryoprotectant kinetic analysis and theoretically shorten the ...
Yu, Hana +5 more
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L-proline: a highly effective cryoprotectant for mouse oocyte vitrification
Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells.
Jin, Xiao-Hu +9 more
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