Results 21 to 30 of about 29,937 (163)

Strategy to Select an Appropriate Cryoprotectant for an X‑ray Study of Escherichia coli GAPDH Crystals

, 2023
The treatment of protein crystals with an appropriate cryoprotectant and immediate freezing in liquid nitrogen before data collection at the synchrotron source is one of the critical steps for determining the structure of three-dimensional ...
Yong Ju Kim, Hui-Woog Choe, Sandi Hnit San Hlaing, Kui-Jae Lee, Jung Hee Park, Jung Hee Park (8054291), Kiyoung Jang, Nahyun Kim (752460), Hyun Gi Kim, Nahyun Kim, Kiyoung Jang (11760868), Hui-Woog Choe (374831), Hyun Gi Kim (16940356), Sandi Hnit San Hlaing (16940353), Yong Ju Kim (11760874), Kui-Jae Lee (16940359)   +15 more
core   +1 more source

Simple Cryoprotectant-Free Method to Advance Pulsed Dipolar ESR Spectroscopy for Capturing Protein Conformational Ensembles

, 2022
Double electron–electron resonance (DEER) is a powerful technique for studying protein conformations. To preserve the room-temperature ensemble, proteins are usually shock-frozen in liquid nitrogen prior to DEER measurements.
Chien-Lun Hung, Te-Yu Kao, Su Wei Lee, Chien-Lun Hung (11192101), Su Wei Lee (11192104), Yun-Wei Chiang, Yu-Jing Lan, Te-Yu Kao (9415584), Yu-Jing Lan (2543167), Yun-Wei Chiang (428651)   +9 more
core   +1 more source

An Update of Oocyte Vitrification: A Modification of Sucrose and Trehalose as Extracellular Cryoprotectant

, 2018
As well as the development of assisted reproductive technology (ART), as the current treatment of woman who failed in achieving pregnancy, the development of an advance vitrification method also grows rapidly.
Ria Margiana, Silvia W Lestari, Nurin N. Fitriyah   +2 more
core   +1 more source

p values for membrane integrities without cryoprotectant removal, and after different methods of cryoprotectant removal when 5% glycerol (two hours) was the cryoprotectant.

p values for membrane integrities without cryoprotectant removal, and after different methods of cryoprotectant removal when 5% glycerol (two hours) was the cryoprotectant.
Elham Ashrafi (8779306), Milica Radisic (161518), Janet A. W. Elliott (7422986)   +2 more
core   +1 more source

p values for membrane integrities without cryoprotectant removal, and after different methods of cryoprotectant removal when 5% Me₂SO + 6% HES was the cryoprotectant.

p values for membrane integrities without cryoprotectant removal, and after different methods of cryoprotectant removal when 5% Me2SO + 6% HES was the cryoprotectant.
Elham Ashrafi (8779306), Milica Radisic (161518), Janet A. W. Elliott (7422986)   +2 more
core   +1 more source

CORELATION BETWEEN DURATION TIMES OF CRYOPROTECTANT TOWARD MICE EMBRYO DEVELOPMENT [PDF]

, 2012
1,2-Propanediol is considerably less toxic than ethylene glycol, moreover it is used as a cryoprotectant. Purpose of the research was find correlation between duration timcs ol' c ryoprotectanl toward mice cmbryos development.
Soeharsono, Bambang Poernomo S., Trianto Nur Abdullah   +2 more
core  

Flash Cooling Protein Crystals: Estimate of Cryoprotectant Concentration Using Thermal Properties

, 2016
X-ray diffraction from protein crystals is routinely measured at cryogenic temperatures, primarily to minimize radiation damage. Protein crystals and the surrounding mother liquor have high water content, which can lead to ice formation when samples are ...
Stephen J. Tomanicek (1434778), Binal N. Shah (2203390), Unmesh Chinte (2203387), Constance A. Schall (2203393), B. Leif Hanson (2203384)   +4 more
core   +1 more source

embryos after vitrification–thawing in high‐osmolarity cryoprotectant solution

, 2018
We aimed to vitrify embryos of Prochilodus lineatus in a high-osmolarity cryoprotectant solution, evaluating, after the vitrification-thawing process, their morphological changes.
Alexandre Ninhaus‐Silveira, José Augusto Senhorini, Rosicleire Verissimo‐Silveira, Siqueira-Silva, Diogenes Henrique de, Ribeiro, Cristiele da Silva [UNESP], Raphael da Silva Costa, Caio de Souza Capuzzo, Senhorini, Jose Augusto, Diógenes Henrique de Siqueira‐Silva, Capuzzo, Caio de Souza [UNESP], Cristiele da Silva Ribeiro, Ninhaus-Silveira, Alexandre [UNESP], Costa, Raphael da Silva [UNESP], Verissimo-Silveira, Rosicleire [UNESP]   +13 more
core   +1 more source

Water activity as an indicator of cryoprotectant binding with erythrocytes

, 2010
За допомогою вдосконаленої моделі кріоскопічного осмометра, який дозволяє визначати активність води в біологічних середовищах з точністю ±0,001 Aw, досліджено зміни активності води в суспензіях еритроцитів коня після додавання кріопротекторів 1,2 ...
Зинченко, В.Д., Кучков, В.Н.   +1 more
core   +1 more source

Effect of different methods of cryoprotectant removal on cell recovery.

H9c2 suspension was cooled at 1°C/min to –40°C in the presence of either 5% Me2SO + 6% HES (incubated for 15 minutes at 0°C) or 5% glycerol (incubated for two hours at room temperature).
Elham Ashrafi (8779306), Milica Radisic (161518), Janet A. W. Elliott (7422986)   +2 more
core   +1 more source