Results 281 to 290 of about 48,476 (320)

Cryoprotectant-treated myocardium evaluation

Cryobiology, 1976
Whole excis ed rat hearts were treated with 5, 10, or 15% (v/v) dimethyl sulfoxide/glycerol and then some were frozen in liquid nitrogen while the balance remained unfrozen. Freezing and thawing rates were approximately 30degreesC/min. Ventricular tissue was examined for histological damage, glycogen depletion, and enzyme sites, using histological ...
L J, Ramazzotto, R, Engstrom
openaire   +2 more sources

Cryoprotectant toxicity neutralization

Cryobiology, 2010
Cryoprotectant toxicity is a fundamental limiting factor for the successful cryopreservation of living systems by both freezing and vitrification, and the ability to negate it would be attractive. Past attempts to demonstrate "cryoprotectant toxicity neutralization" (CTN) have had many ups and downs. First convincingly introduced by Baxter and Lathe in
openaire   +2 more sources

Microwave properties of cryoprotectants

Cryobiology, 1978
Abstract The values of the dielectric constant and of the loss tangent for pure samples of DMSO, ethylene glycol, and glycerol were determined over the temperature range of + 15 to −70 °C. An operating frequency range of 1.45 to 1.55 GHz was used, allowing direct application of the results of both 0.915 and 2.450 GHz studies.
J D, Macklis, F D, Ketterer
openaire   +2 more sources

Aseptic capillary vitrification of human spermatozoa: Cryoprotectant-free vs. cryoprotectant-included technologies

Cryobiology, 2021
The protocol of aseptic cryoprotectant-free vitrification on human spermatozoa is well documented. However, data about the effect of permeable cryoprotectants at this procedure is limited. Presented study aimed to test the aseptic capillary vitrification technologies using permeable cryoprotectant-included or cryoprotectant-free media.
Mengying, Wang, Plamen, Todorov, Evgenia, Isachenko, Gohar, Rahimi, Wanxue, Wang, Melanie, von Brandenstein, Pradeep, Kumar, Peter, Mallmann, Vladimir, Isachenko   +8 more
openaire   +2 more sources

Cryoprotectant toxicity and cryoprotectant toxicity reduction: In search of molecular mechanisms

Cryobiology, 1989
Cryoprotectant toxicity is a fundamental obstacle to the full potential of artificial cryoprotection, yet it remains in general a poorly understood phenomenon. Unfortunately, most relevant biochemical studies to date have not met the basic criteria required for demonstrating mechanisms of toxicity.
G M, Fahy, T H, Lilley, H, Linsdell, M S, Douglas, H T, Meryman   +4 more
openaire   +2 more sources

Unloading of cryoprotectants from cryoprotectant-loaded cells on a microfluidic platform

Biomedical Microdevices, 2017
In this paper, a multistep dilution-filtration microdevice (MDFD) is developed for unloading cryoprotectants from cryoprotectant-loaded cells. The MDFD contained a diluent producing region, a dilution-filtration execution region, and a filtrate collection region.
Yufeng, Kang, Lili, Zou, Bensheng, Qiu, Xin, Liang, Sijie, Sun, Dayong, Gao, Weiping, Ding   +6 more
openaire   +2 more sources

Cryopreservation of stallion spermatozoa using different cryoprotectants and combinations of cryoprotectants

Animal Reproduction Science, 2015
The present study investigates the effects of five cryoprotectants (CPAs) and cryoprotectant combinations on the post-thaw total motility, progressive motility, viability, mitochondrial membrane potential and acrosome integrity in stallion spermatozoa. In Experiment 1, the objective was to compare the impact of different concentrations (2.5%, 3.5% and ...
Zhuangyuan Wu, Xinbiao Zheng, Yongming Luo, Fei Huo, Hong Dong, Guoting Zhang, Weihao Yu, Fang Tian, Liangjun He, Jingbo Chen   +9 more
openaire   +2 more sources

Bacterial cryoprotectants

Resonance, 2002
Cold-adapted organisms are known to accumulate cryoprotective substances inside their cells. Glycine betaine is a bacterial cryoprotectant. It is believed to act by stabilizing cellular proteins and membranes at low temperature.
openaire   +1 more source

Cryoprotective properties of vasoconstriction

Cryobiology, 1974
Abstract In standardized freezing experiments made on depilated rat skin, it was found that vasconstriction gives some cryoprotection, but, only if tissue temperatures do not drop below −30 °C. It is suggested that vasoconstriction, which is a well-known sequel to more moderate drops in tissue temperature, is in fact a physiological temperature ...
openaire   +2 more sources

Enzyme activity of cryoprotected myocardium

Cryobiology, 1976
Abstract The use of in vivo intravenously infused DMSO/glycerol in Krebs-Henseleit buffer solution as a cryoprotectant in rat left ventricles was investigated. Alkaline phosphatase and creatine phosphokinase activities were monitored. Data show inhibition of alkaline phosphatase by the cryoprotectant solution.
L J, Ramazzotto, R, Carlin, R, Engstrom
openaire   +2 more sources